The invention discloses a composition capable of preventing hangover, dispelling the effects of alcohol and protecting the liver and a preparation method of the composition. The composition comprises ampelopsis grossedentata leaf instant powder, soybean peptide powder, a yeast extract, herba cepbalanoplosis segeti powder, roxburgh rose fruit powder, dandelion powder, corn stigma powder, moringa oleifera leaf powder, vitamin C, sorbitol DC, corn starch and magnesium stearate. By enhancing the metabolic capacity of the body and accelerating decomposition and oxidation of alcohol and metabolites thereof, damage of alcohol and acetaldehyde to liver cell membranes and mitochondrial membranes is reduced, and liver cells are protected, so that various long-term and short-term discomfort such as dizziness, dizziness, nausea, vomiting and the like after drinking is relieved, damage of alcohol to the human body is reduced to a great extent. The alcohol effect dispelling preparation not only has the effect of improving the alcohol content, but also has a remarkable effect on dispelling effects of alcohol andprotecting the, and can be prepared into various dosage forms, such as tablets, powder and drinks.