30 results about "Quinone oxidoreductase" patented technology

Disclosed herein are various bladder cancer treatments and methods. The present disclosure can take advantage of propylene glycol concentrations and / or NAD(P)H:quinone oxidoreductase-1 (NQO1), Cytochrome P450 Oxidoreductase (P450R) and Glucose transporter 1 (Glut-1) protein expression in human transitional cell carcinoma of the bladder to offer individually targeted bladder cancer treatments.

The present invention includes a probe, an assay, a method of detecting, a human NAD(P)H quinone oxidoreductase-1 (hNQO1) enzyme activity with a fluorescent probe comprising a quinone propionic acid (QPA) conjugated to dicyanoisophorone (DCP), wherein the hNQO1 reduces the probe to releases a fluorescent DCP, and a method of making the same.

The invention discloses an NQO (quinone oxidoreductase) response type near infrared fluorescence probe compound related to early diagnosis of tumor. The structure of the compound is represented as theformula (I), wherein R1 and R2 are the same or different, and R1 and R2 are independently selected from C1-6 linear alkyl, C1-6 linear alkyl carboxyl, sulfonyl or C1-6 linear alkyl ester; X is halogen. The primary cell confocal experiment of the compound proves that the compound has good NQO response capacity, can produce strong fluorescence easy to detect, and can be applied to early detection of tumor.

Provided is a method capable of effectively treating various diseases associated with energy excess, such as obesity, diabetes, metabolic syndromes, degenerative diseases and mitochondrial dysfunction-related diseases, via elevation of an NAD(P)+ / NAD(P)H ratio by increasing an NAD(P)+ concentration in vivo or in vitro through use of NAD(P)H as a substrate or coenzyme by oxidoreductase such as NAD(P)H:quinone oxidoreductase (NQO1), a method of screening a drug for the same and a therapeutic drug.

The invention relates to a preparation method of a ginger extract with detoxifying effect on exogenous carcinogens and a ginger extract product, and belongs to the technical field of deep processing of ginger. The preparation method comprises the following steps of: selecting and cleaning fresh ginger serving as a raw material, performing freeze drying, crushing, extracting with ethanol solution,and performing concentration to obtain ginger extract; performing ethyl acetate / aqueous phase separation, collecting ethyl acetate layer, concentrating and drying, and thus obtaining the ethyl acetate extract; and finally, performing extraction by silica gel column chromatography, performing gradient elution by using ethyl acetate / n-hexane system or the like, collecting elution components of 30to 70 percent ethyl acetate eluent, performing vacuum concentration, volatilizing the residual solvent by using a nitrogen blowing instrument, packing and sealing, and thus obtaining the ginger extract. The extract can remarkably induce the activity of quinone oxidoreductase (QR) in rat liver cancer Hepa1c1c7 cells, shows good potential of up-regulated II phase detoxifying enzyme, and can be usedfor developing low-toxicity efficient products such as cancer preventing agent, functional food and the like.

The invention discloses a primer and a kit for detecting NQO1 gene polymorphism, and an application of primer and kit on pathological examination. The primer comprises a specific primer for amplifying the fourth exon C465T site fragment and the sixth exon C609T site fragment of quinone oxidoreductase 1 gene and a primer for pyrosequencing the obtained nucleic acid fragment. The polymorphism of the fourth exon C465T site fragment and the sixth exon C609T site fragment of quinone oxidoreductase 1 gene related to tumor susceptibility can be detected by adopting the pyrosequencing technology through the primer, method and kit disclosed by the invention, the specificity is good and the accuracy is high.

Methods of treating lung diseases comprising administering inducers of NAD(P)H:quinone oxidoreductase 1 (NQO1) are disclosed. Inducers of NQO1 include naphthoquinones such as β-lapachone. Methods of predicting whether a subject with a lung disease will respond to treatment with a naphthoquinone are also described herein.

The invention discloses a method for removing hydrogen sulfide by utilizing heterotrophic microorganisms. Specific heterotrophic microorganisms are added in a device for removing hydrogen sulfide, an aerobic reaction is performed in a set reaction system, and removal on H2S, HS- or / and S2- in the reaction system of the device is implemented, wherein the heterotrophic microorganisms mean a category of heterotrophic bacteria of which a genome comprises a sulfide:quinone oxidoreductase gene and a persulfid dioxygenase gene and which can oxidize sulfides to generate zero-valent sulphur and thiosulfate, or sulfate. According to the method disclosed by the invention, the screened heterotrophic bacteria have efficient capacity of oxidize exogenous hydrogen sulfide, has the characteristics of high growth speed, low culture cost and capacity of forming great biomass, effectively solves the problem of condition limitation in the prior art in which autotrophic bacteria are used for remove hydrogen sulfide, and has a great application potential in the industrial production.

The invention provides a bionic nano-carrier with dual functions of oxidation and anti-oxidation, and a preparation method and an application thereof. The bionic nano-carrier comprises an erythrocytemembrane and an organic-metal framework coated in the erythrocyte membrane, and the organic-metal framework is loaded with functionalized SOD enzyme and beta-lapaquone. After the bionic nano-carrier reaches the acidic environment of tumor cells, metal-ligand bonds in the MOF are hydrolyzed to generate protonated ligands to destroy the erythrocyte membrane and release the enzyme and drug; the beta-lapafenone is catalyzed by quinone oxidoreductase 1 highly expressed by tumor cells to generate a large number of superoxide anions, and is catalyzed by the SOD enzyme to generate hydrogen peroxide, and iron ions in the SOD enzyme catalyze the hydrogen peroxide through a Fenton reaction to generate hydroxyl radicals in order to kill the tumor cells. The bionic nano-carrier provided by the invention does not generate drug resistance through enzyme treatment, can significantly reduce the systemic toxicity caused by drug application, and has extremely high clinical application prospects.

The invention discloses application of indeno triazine compounds as a substrate of quinone oxidoreductase depending on NAD (P) H. The indeno triazine compounds are catalyzed through the quinone oxidoreductase depending on the NAD (P) H to generate an oxidation reduction cycle reaction, a large number of reactive oxide free radicals are generated, strong oxidative stress is induced, and the obvious lethal effect on lung cancer, liver cancer, cervical cancer, pancreatic cancer, breast cancer, prostate cancer, nasopharynx cancer, kidney clear cell carcinoma, oral epidermoid carcinoma, chronic myelogenous leukemia, intestinal cancer, brain cancer, gastric cancer, esophagus cancer and other cancer cells expressed by the quinone oxidoreductase depending on the NAD (P) H is achieved. The indeno triazine compounds used as the substrate of the quinone oxidoreductase depending on the NAD (P) H can also be used as sensitizers for treating the cancer with radiotherapy, and have the obvious anticancer synergistic effect. In addition, the indeno triazine compounds used as the substrate of the quinone oxidoreductase depending on the NAD (P) H have antibacterial and antifungal activity.

A method for screening a compound acting on a bone or its salt characterized by using NRH: quinone oxidoreductase (NQO2), its peptide fragment or a salt thereof; agents acting on a bone comprising compounds obtained by the above method; a method of preventing / treating human or mammalian bone diseases involving the inhibition or promotion of the expression or activity of NQO2; and diagnostics for bone diseases containing a polynucleotide encoding NQO2 or its peptide fragment or an antibody having an affinity specifically for NQO2, its peptide fragment or a salt thereof.

The present disclosure pertains to a genetically modified microorganism that satisfies some of specific requirements. The specific requirements include: (I) compared to a wild type microorganism, having reduced or inactivated succinate dehydrogenase activity or fumarate reductase activity; (II) compared to a wild type microorganism, having reduced or inactivated lactate dehydrogenase activity; (III) having a modified phosphoenolpyruvate carboxylase activity and thus showing resistance against the feedback inhibition by aspartic acid in wild type phosphoenolpyruvate carboxylase activity, or having an exogenous phosphoenolpyruvate carboxylase activity and thus showing higher resistance against the feedback inhibition by aspartic acid than wild type phosphoenolpyruvate carboxylase activity shown by a wild type microorganism; and (IV) compared to a wild type microorganism, having reduced or inactivated pyruvate : quinone oxidoreductase.

Provided is a method capable of effectively treating various diseases associated with energy excess, such as obesity, diabetes, metabolic syndromes, degenerative diseases and mitochondrial dysfunction-related diseases, via elevation of an NAD(P)+ / NAD(P)H ratio by increasing an NAD(P)+ concentration in vivo or in vitro through use of NAD(P)H as a substrate or coenzyme by oxidoreductase such as NAD(P)H:quinone oxidoreductase (NQO1), a method of screening a drug for the same and a therapeutic drug.

The invention relates to the field of microorganisms, in particular to construction of a threonine production strain with a high conversion rate and a method for producing threonine. L-threonine yield of novel escherichia coli is higher than that of respective control strains, and the shake-flask conversion rate of strains with enhanced mqo expression activity is 24-30%, which is increased by 6-12 conversion percentages compared with original strains; the shake-flask conversion rate of strains with enhanced cyoABCDE expression activity is 26-30%, which is also increased by 8-12 conversion percentages compared with the original strains. Results of shake flasks modified by the two sites show that the production capacity of threonine can be obviously improved by enhancing the expression of malic acid:quinone oxidoreductase gene mqo or enhancing the activity of oxidase at the tail end of a respiratory chain.

The invention relates to application of Wangshi Baochi pills in preparation of a medicine for preventing and / or treating alcoholic liver injury, and belongs to the technical field of medicines. The application of the Wangshi Baochi pills in preparation of the medicine for preventing and / or treating alcoholic liver injury is provided, and researches find that the Wangshi Baochi pills can remarkably shorten the sobering time of drunken mice and reduce the levels of glutamic oxalacetic transaminase and glutamic-pyruvic transaminase in serum of acute alcoholic liver injury modeling mice, can significantly improve the pathological change of liver tissue of the acute alcoholic liver injury modeling mice, can significantly reduce the expression of active oxygen in the liver tissue of the acute alcoholic liver injury modeling mice, and can significantly improve the activity of superoxide dismutase and the level of glutathione in the liver tissue of the acute alcoholic liver injury modeling mice, and in addition, the Wangshi Baochi pills can increase the expression of quinone oxidoreductase in liver tissue of the acute alcoholic liver injury modeling mice and have a good alcoholic liver injury relieving effect.

Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding quinone oxidoreductase proteins, polypeptides encompassing quinone oxidoreductase proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

The invention discloses an immobilized heterotrophic microorganism for removing sulfides. The immobilized heterotrophic microorganism is prepared by wrapping a heterotrophic microorganism of which a genome comprises a sulfur quinone oxidoreductase gene and a sulfide dioxygenase gene in alginic acid latex spheres with a support function, and meanwhile adding a set amount of fe3o4 nanoparticles. The invention further discloses application of the immobilized heterotrophic microorganism in removing sulfides in wastewater or waste gases under an aerobic reaction condition. Tests show that introduction of sulfides and nutritive substances is not blocked by the cell immobilization method disclosed by the invention, heterotrophic microorganisms can be bred in the sulfides and the nutritive substances, and thus the sulfide oxidation efficiency of the immobilized cells of a unit volume is improved in the culture process; in addition, acidification problems are avoided in the sulfide removal process, conventional difficulties of bioremediation of sulfides are effectively solved, and the immobilized heterotrophic microorganism has the characteristics of repeated use, low culture cost, high sulfide endurance and the like.

The invention discloses a method for removing hydrogen sulfide by utilizing heterotrophic microorganisms. Specific heterotrophic microorganisms are added in a device for removing hydrogen sulfide, an aerobic reaction is performed in a set reaction system, and removal on H2S, HS- or / and S2- in the reaction system of the device is implemented, wherein the heterotrophic microorganisms mean a category of heterotrophic bacteria of which a genome comprises a sulfide:quinone oxidoreductase gene and a persulfid dioxygenase gene and which can oxidize sulfides to generate zero-valent sulphur and thiosulfate, or sulfate. According to the method disclosed by the invention, the screened heterotrophic bacteria have efficient capacity of oxidize exogenous hydrogen sulfide, has the characteristics of high growth speed, low culture cost and capacity of forming great biomass, effectively solves the problem of condition limitation in the prior art in which autotrophic bacteria are used for remove hydrogen sulfide, and has a great application potential in the industrial production.

The invention discloses a quinone oxidoreductase (NQO1) targeted indoquinone 23-hydroxy betulinic acid derivative and a preparation method and application thereof. The compound is good in activity and small in toxicity, has a remarkable inhibition effect on tumor cell growth, particularly has a better inhibition effect on NQO1 high-expression tumor cells, and can be used for further preparing novel anti-tumor drugs. The preferably treated tumor diseases are lung cancer, liver cancer, melanoma and colon cancer.

The invention relates to a preparation method of a ginger extract with detoxifying effect on exogenous carcinogens and a ginger extract product, and belongs to the technical field of deep processing of ginger. The preparation method comprises the following steps of: selecting and cleaning fresh ginger serving as a raw material, performing freeze drying, crushing, extracting with ethanol solution,and performing concentration to obtain ginger extract; performing ethyl acetate / aqueous phase separation, collecting ethyl acetate layer, concentrating and drying, and thus obtaining the ethyl acetate extract; and finally, performing extraction by silica gel column chromatography, performing gradient elution by using ethyl acetate / n-hexane system or the like, collecting elution components of 30to 70 percent ethyl acetate eluent, performing vacuum concentration, volatilizing the residual solvent by using a nitrogen blowing instrument, packing and sealing, and thus obtaining the ginger extract. The extract can remarkably induce the activity of quinone oxidoreductase (QR) in rat liver cancer Hepa1c1c7 cells, shows good potential of up-regulated II phase detoxifying enzyme, and can be usedfor developing low-toxicity efficient products such as cancer preventing agent, functional food and the like.

The invention relates to a neuroglioma protein marker and a purpose therefor. The invention relates to the utilization of human neuroglioma differential protein obtained by a rat model in which the right side caudate nucleus of the rat is injected with neuroglioma cells and mass spectrometry as a latent neuroglioma marker for the purpose of early stage diagnosis, and condition monitoring. The marker includes: a glutamyl amino peptidase, an amino-peptidase N, a glutathione synthetase, neutral and alkaline amino acid transportation protein, calmodulin, a solute carrier household 7 member 13, a gamma-glutamyl transpeptidase 1, an organic anion transportation carrier family, a glutamic acid-cysteine ligase regulation subunit, an EH structural domain protein 1, a cystathionine gamma-lyase, a quinone oxidoreductase and the like. The selection of the more simple, faster, and more noninvasive method for diagnosing the neuroglioma is provided.

The invention provides a noninvasive detection kit for susceptibility genes for multiple myeloma. The kit comprises specific primers, DNA (deoxyribonucleic acid) sequencing primers, PCR (polymerase chain reaction) components, PCR product purification components, DNA sequencing reaction components and the like for detecting genotypes of four single nucleotide polymorphism (SNP) sites, including a C677T site polymorphism and A1298C site polymorphism on a methylene tetrahydrofolate reductase (MTHFR) gene, C609T site polymorphism on quinone oxidoreductase 1 (NQO1) gene, and present / null site polymorphism of a glutathione s-transferase theta 1 (GSTT1) gene. According to the invention, genotypes of four SNP sites closely related with the multiple myeloma occurrence are detected by the kit, so as to estimate the risk level of the multiple myeloma of a subject, and finally the subject is instructed at a genetic level to specifically prevent occurrence of multiple myeloma according to a gene detection result of each subject so as to lower the morbidity of the multiple myeloma.

Disclosed herein are various bladder cancer treatments and methods. The present disclosure can take advantage of propylene glycol concentrations and / or NAD(P)H:quinone oxidoreductase-1 (NQO1), Cytochrome P450 Oxidoreductase (P450R) and Glucose transporter 1 (Glut-1) protein expression in human transitional cell carcinoma of the bladder to offer individually targeted bladder cancer treatments.

The invention provides a biomimetic nano-carrier with dual functions of oxidation and anti-oxidation, a preparation method and application thereof. The biomimetic nano-carrier provided by the invention comprises red blood cell membrane and an organometallic framework coated in the red blood cell membrane, and the organometallic framework is loaded with functionalized SOD enzyme and β-lapachone. After the biomimetic nanocarrier reaches the acidic environment of tumor cells, the metal-ligand bond in the MOF is hydrolyzed to produce protonated ligands that destroy the red blood cell membrane, and the enzyme and drug are released; β-lapachone is oxidized and reduced by the quinone highly expressed in tumor cells Enzyme 1 catalyzes the production of a large amount of superoxide anion, which is catalyzed by SOD enzyme to produce hydrogen peroxide. Finally, iron ions in SOD enzyme catalyze hydrogen peroxide to produce hydroxyl free radicals through Fenton reaction, thereby killing tumor cells. The biomimetic nano-carrier provided by the invention not only does not produce drug resistance through enzyme treatment, but also can significantly reduce systemic toxicity caused by drug administration, and has a very high clinical application prospect.

Provided is a Pichia pastoris mutant strain for expressing an exogenous gene. Specifically, provided is a Pichia pastoris mutant strain comprising, with respect to Pichia pastoris mutant strain GS115 or CICC32806, one or more of the following six mutations: BQ9382_C1-2260, EKK deletions at positions 308-310, a hypothetical protein; BQ9382_C1-3800, E129K, 60S ribosomal subunit assembly / exported protein LOC1; BQ9382_C1-5700, I312M, mitochondrial external NADH dehydrogenase, type II NAD(P)H:quinone oxidoreductase; BQ9382_C2-3950, Q145X, an essential protein having a binding partner Psr1p and used for completely activating a general stress response; BQ9382_C3-2220, E188K, a hypothetical protein; and BQ9382_C3-4370, W196X, orotidine 5\′-phosphate decarboxylase. The provided Pichia pastoris mutant strain is an effective commonly employed host for exogenous expression, and can efficiently express different proteins, especially phospholipase and lipase.

The invention provides application of tangeretin in the preparation of products to activate ability of cells to resist oxidative stress. The products include functional foods, health products or drugs. Tangeretin can improve evidently the ability of human liver L02 cells to resist hydrogen peroxide oxidative stress injury, can enhance the expression of antioxidase quinone oxidoreductase NQO1 and catalase CAT by adjusting oxidative stress-related Nrf2-Keap1-Cul3 pathway, and can inhibit Ubiquitylation degradation of Nrf2 by inhibiting the expression of Cul3 so as to maintain the ability of L02 cells to resist oxidative stress. Therefore, tangeretin can act as a functional food, health product or preventive drug to prevent and treat hepatic oxidative stress injury related diseases.

The invention is a gene coding for a quinone oxidoreductase from Kluyveromyces marxianus and a protein having an amino acid sequence expressed therefrom, which can be advantageously used in a reduction reaction of a quinone compound and synthesis of intermediates for a biologically active compound.

The invention discloses quinone oxidoreductase (NQO1)-targeted indole quinone 23-hydroxybetulinic acid derivatives, a preparation method and application. The compound of the invention has good activity and low toxicity, has significant inhibitory effect on tumor cell growth, especially has better inhibitory effect on tumor cells with high expression of NQO1, and can be used to further prepare new anti-tumor drugs. Preferred neoplastic diseases for treatment are lung cancer, liver cancer, melanoma and colon cancer.