A neuroglioma protein marker and a purpose therefor

A glioma, protein technology, applied in the field of protein markers

Inactive Publication Date: 2018-08-31
BEIJING NORMAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, imaging information does not provide valuable diagnostic clues earlier in the disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A neuroglioma protein marker and a purpose therefor
  • A neuroglioma protein marker and a purpose therefor
  • A neuroglioma protein marker and a purpose therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Establishment of glioma rat model

[0046] In the present invention, glioma cell C6 was injected into the right caudate nucleus of rats to simulate the pathophysiological state of glioma. The present invention collects the urine of glioma rats in the early stage (2 days, 6 days after operation) and middle and late stage (10 days, 13 days after operation). Using this animal model to simulate glioma has important guiding significance for clinical early diagnosis, treatment and prognosis of glioma.

[0047] 1. Materials and reagents

[0048] 1) Instrument: Rat urine collection tube: purchased from BD Company. Thermo orbitrap fusion lumos mass spectrometer: purchased from Thermo Fisher Scientific; Thermo EASY-Nlc1200 high-performance liquid chromatography: purchased from ThermoFisher Scientific; MillliQ RG ultrapure water system: purchased from Millipore; C18 reversed-phase analytical column (RP column ,0.1×150mm,3μm, ): purchased from Michrom Bioresource...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a neuroglioma protein marker and a purpose therefor. The invention relates to the utilization of human neuroglioma differential protein obtained by a rat model in which the right side caudate nucleus of the rat is injected with neuroglioma cells and mass spectrometry as a latent neuroglioma marker for the purpose of early stage diagnosis, and condition monitoring. The marker includes: a glutamyl amino peptidase, an amino-peptidase N, a glutathione synthetase, neutral and alkaline amino acid transportation protein, calmodulin, a solute carrier household 7 member 13, a gamma-glutamyl transpeptidase 1, an organic anion transportation carrier family, a glutamic acid-cysteine ligase regulation subunit, an EH structural domain protein 1, a cystathionine gamma-lyase, a quinone oxidoreductase and the like. The selection of the more simple, faster, and more noninvasive method for diagnosing the neuroglioma is provided.

Description

technical field [0001] The invention relates to clinical medicine, in particular to human glioma-related protein markers. Specifically, the present invention relates to the use of protein markers related to the diagnosis and disease monitoring of human glioma obtained by using a rat glioma model and mass spectrometry analysis. Background technique [0002] Urine proteomics is of great significance in disease research. Markers are measurable changes associated with physiological and pathophysiological states. Urine is not regulated by homeostasis mechanisms and can reflect various early changes, so it is a good place to look for early biomarkers of diseases (see Youhe G. Can urine be the gold mine for biomarker discovery? Sci China Life Sci 2013; 43) . [0003] While the central nervous system is metabolizing at a high speed, it will metabolize the metabolites produced in the direction of decreasing the steady-state level. Metabolic waste products can be excreted into the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/68G01N33/57407G01N33/57488G01N2800/28G01N2800/56
Inventor 高友鹤倪艳颖张繁霜
Owner BEIJING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap