Health-care product for relieving alcohol and preventing getting drunk
A product and a technology of action, applied in the field of health care products and their preparation, can solve the problems of poor hangover effect and the like
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Embodiment 1
[0031] Take the preparation of 1000 conventional capsules of the product of the present invention as an example:
[0032] Acetobacter pasteurianus subsp. Pasteurianus (Acetobacter pasteurianus subsp. Pasteurianus, AS1.1550), inoculated in a liquid medium (the medium consists of 100g of glucose, 10g of yeast extract, 20g of calcium carbonate, 18-20g of agar, 1L of distilled water, pH=6.8 , 20 milliliters of 95% ethanol), cultivated at 30°C, with a shaking cycle of 80rpm, after cultivating for 48 hours, using blank medium as a control, the culture solution OD 600= 1.0, the culture solution was centrifuged at 3000g, the thallus was collected, 0.05M phosphate buffer solution of 10 times the amount of pH=6.5 was added, homogenized at 12000rpm in a high-speed homogenizer for 5 minutes, the homogenate was centrifuged at 3000g for 20min, and the precipitate was discarded to obtain Supernatant, ferric sulfate, an enzyme protection agent, was added to the supernatant to make the concent...
Embodiment 2
[0036] Take the preparation of 1000 conventional capsules of the product of the present invention as an example:
[0037] Acetobacter pasteurianus subsp. Pasteurianus (Acetobacter pasteurianus subsp. Pasteurianus, AS1.1550), inoculated in a liquid medium (the medium consists of 100g of glucose, 10g of yeast extract, 20g of calcium carbonate, 18-20g of agar, 1L of distilled water, pH=6.8 , 20 milliliters of 95% ethanol), cultivated at 30°C, with a shaking cycle of 80rpm, after cultivating for 48 hours, using blank medium as a control, the culture solution OD 600 = 1.0, the culture solution was centrifuged at 3000g, the thallus was collected, 0.05M phosphate buffer solution of 10 times the amount of pH=6.5 was added, homogenized at 12000rpm in a high-speed homogenizer for 5 minutes, the homogenate was centrifuged at 3000g for 20min, and the precipitate was discarded to obtain Supernatant, ferric sulfate, an enzyme protection agent, was added to the supernatant to make the concen...
Embodiment 3
[0045] Take the preparation of 1000 conventional capsules of the product of the present invention as an example:
[0046] Acetobacter pasteurianus subsp. Pasteurianus (Acetobacter pasteurianus subsp. Pasteurianus, AS1.1550), inoculated in a liquid medium (the medium consists of 100g of glucose, 10g of yeast extract, 20g of calcium carbonate, 18-20g of agar, 1L of distilled water, pH=6.8 , 20 milliliters of 95% ethanol), cultivated at 30°C, with a shaking cycle of 80rpm, after cultivating for 48 hours, using blank medium as a control, the culture solution OD 600 = 1.0, the culture solution was centrifuged at 3000g, the thallus was collected, 0.05M phosphate buffer solution of 10 times the amount of pH=6.5 was added, homogenized at 12000rpm in a high-speed homogenizer for 5 minutes, the homogenate was centrifuged at 3000g for 20min, and the precipitate was discarded to obtain Supernatant, add enzyme protection agent ferric sulfate to the supernatant to make the concentration 10m...
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