63 results about "Ethanol metabolism" patented technology

The invention relates to a composition with disintoxicating and liver-protecting effects, which comprises the following raw materials in percentage by mass: 0.15 to 5 percent of crucumin, 0.04 to 1.0 percent of zinc lactate, 10 to 50 percent of glutamine, 10 to 50 percent of alanine and 10 to 50 percent of xylooligosaccharide. It is proved that, by an animal experiment and a human tasting experiment, the composition increases the alcohol dehydrogenase and acetaldehyde dehydrogenase content of a body and rapidly decomposes alcohol, so that the composition has the effects of promoting alcohol metabolism in a liver and reducing the alcohol concentration and active oxygen concentration in a blood; and the composition increases an endurance capacity of a human body to the alcohol, reduces the discomforts such as headache, nausea, vomiting and the like due to the alcohol, and reduces the damages of the alcohol to the liver. The composition can be prepared into food and health-care food with the disintoxicating and liver-protecting effects, can be used as a disintoxicating product for preventing and improving excessive drinking, and can be used as an adjuvant therapy product for an alcoholic liver, a fatty liver due to the alcohol or non-alcohol and protecting the liver; and the composition has the advantages of safety, non-toxicity and a reliable curative effect.

The invention discloses a compound prepared by probiotic-fermented traditional Chinese medicine (TCM) preparation as well as a preparation method and application of the compound. The compound takes a culture solution containing crude TCM drugs such as astragalus, semen hoveniae, flower of kudzuvine, radix paeoniae alba and ligusticum wallichii, and takes fermentation liquor fermented by probiotics, such as bifidobacterium bifidum, lactobacillus acidophilus and bacillus subtillis, as an effective ingredient. The compound disclosed by the invention can be used for promoting ethanol metabolism in the liver, lowering ethanol concentration in blood, enhancing the endurance capacity of a human body to alcohol, and alleviating discomforts of head and face redness, dizziness and the like caused by alcohol. Foods and healthcare foods prepared by the compound disclosed by the invention can be taken as safe and reliable anti-alcoholic products for preventing and alleviating excessive drinking.

The invention discloses a 3-methylquinoxaline-2-carboxylic acid artificial antigen and an antibody obtained by the 3-methylquinoxaline-2-carboxylic acid artificial antigen. The invention provides a compound shown in the formula (I). The compound shown in the formula (I) is prepared by structural modification of 3-methylquinoxaline-2-carboxylic acid, retains a feature structure of 3-methylquinoxaline-2-carboxylic acid as much as possible and has active groups which can couple with a carrier protein. The 3-methylquinoxaline-2-carboxylic acid artificial antigen provided by the invention is a conjugate prepared by coupling of the compound shown in the formula (I) and a carrier protein. The 3-methylquinoxaline-2-carboxylic acid artificial antigen is used for animal immunization so that high-specificity monoclonal and polyclonal antibodies are obtained. A preparation method of the high-specificity monoclonal and polyclonal antibodies is simple and practicable. The 3-methylquinoxaline-2-carboxylic acid artificial antigen can be used for detection of olaquindox metabolites. The antibodies obtained by the 3-methylquinoxaline-2-carboxylic acid artificial antigen can be used for detection of olaquindox metabolites.

The invention relates to the technical field of bioengineering, and in particular relates to a saccharomyces cerevisiae engineering strain as well as a preparation method, application and a fermentation culture method thereof. The PDC1, PDC5, PDC6, ADH1 and ADH4 genes of the saccharomyces cerevisiae engineering strain are knocked out, and an LDH gene is inserted into the saccharomyces cerevisiae engineering strain. The saccharomyces cerevisiae engineering strain provided by the invention does not contain an ethanol metabolism main route, a lot of glucose can be used for fermentation so as to accumulate lactic acid, and the strain can grow quickly by using ethanol.

The present invention discloses one kind of Saccharomyces cerevisiae strain with glycerin passage protein gene deficiency and resulting in lowered glycerin generation and raised ethanol yield and its construction process. The construction process includes the following steps: 1. obtaining Saccharomyces cerevisiae monoploid and deleting of URA3 gene; 2. constructing PUC18-FPS1-ORF plasmid; 3. constructing PUC18-FUF plasmid; and 4. constructing KAM-21 strain. The present invention deletes glycerin passage protein FPS1 gene code embedded on cell membrane and loses the expression of FPS1 gene by means of gene engineering technology, so as to inhibit glycerin synthesizing way, convert from glycerin metabolism to ethanol metabolism, increase ethanol yield, and solve serial problems in ethanol production.

The invention discloses an amygdalus persica/amygdalus davidiana resin polysaccharide embedded natural antioxidant composition and an application thereof. The composition can effectively prevent intoxication caused by single-time heavy-dosage wine drinking and relevant organism health risks after intoxication. The composition is prepared through the following steps of in an aqueous solution system, using amygdalus persica/amygdalus davidiana resin polysaccharide as a wall material, and performing micro nanocrystallization embedding on a natural antioxidant so as to obtain the composition. Preferably, in the composition, the mass concentration of the amygdalus persica/amygdalus davidiana resin polysaccharide is not greater than 5%g/mL, and the optimal mass concentration is 1%-2.5%g/mL. According to the amygdalus persica/amygdalus davidiana resin polysaccharide embedded natural antioxidant composition disclosed by the embodiment of the invention, the amygdalus persica/amygdalus davidianaresin polysaccharide forms a protective barrier resistant to digestive enzyme decomposition in gastrointestinal tracts, so that chemical damage of high-concentration ethanol to stomach and intestinemucosa can be avoided or reduced; and natural antioxidant molecules through micro nanocrystallization embedding of the amygdalus persica/amygdalus davidiana resin polysaccharide can restrain oxidativestress cascading damage caused by ethanol metabolism, the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase in bodies can be activated and increased, and ethanol metabolism can be promoted.

The invention discloses a kit for detecting 3-methyl-quinoxaline-2-carboxylic acid. The enzyme linked immunosorbent assay (ELISA) kit for detecting 3-methyl-quinoxaline-2-carboxylic acid comprises a monoclonal antibody which has an anti-preservation number of CGMCC No.5778 and is excreted by an olaquindox metabolite monoclonal antibody hybrid tumor 5A2. The kit comprises the monoclonal antibody, and a conjugate of a compound and a barrier protein, which is shown as a formula (I). The kit has the advantages of high sensitivity, high accuracy, high precision, low cost, simplicity for operation, short detection time, suitability for use by various units, simplicity for storage and long shelf life, can be used for quickly detecting a large number of samples at the same time, and can realize on-site high-flux quick detection. The ELISA kit is suitable for determining the residual quantity of olaquindox metabolites in feeds, animal tissues or excrements. The antibody, the kit and a detection method can play a major role in detection of the olaquindox metabolites.

The invention discloses an olaquindox metabolite immunochromatography test paper card and a preparation method thereof and belongs to the technical field of veterinary drug residue immunochemistry fast detection. The test paper card comprises a plastic card shell and a test paper strip encapsulated in the plastic card shell. A sample adding hole and an observation window are formed in the plastic card shell. The bottom layer of the test paper strip is a support layer. A sample pad, a colloidal gold pad, a testing pad and a water absorption pad which are connected in a overlap manner are sequentially attached to the support layer from left to right. The test paper card is simple to operate, fast, accurate, high in flexibility, high in specificity, vivid and visualized in result display, and the like. In addition, the test paper card is capable of on-site monitoring and suitable for screening of a huge amount of samples.

The invention discloses olaquindox metabolite hapten, and corresponding artificial antigen and monoclonal antibody. In addition, the invention also discloses a preparation method and applications of the olaquindox metabolite hapten and corresponding artificial antigen and monoclonal antibody. The olaquindox metabolite antigen can be obtained by connecting the olaquindox metabolite hapten with a carrier protein. The olaquindox metabolite antigen can be applied to preparing olaquindox metabolite specific antibody. The preparation method is simple, convenient and feasible, and lower in cost, and the hapten yield is higher. The artificial olaquindox metabolite antigen can produce olaquindox metabolite-targeted specific antibody through immune animal, can be used for preparing an enzyme linked immunosorbent assay kit or a colloidal gold test paper card for detecting the residual olaquindox metabolite in food, and the olaquindox metabolite hapten has various advantages of being simple, fast, large in sample processing quantity, high in sensitivity, strong in specificity and the like.

The invention discloses a detection method for metabolite residues of carbadox and olaquindox in animal tissues. The detection method comprises the following steps: adding internal standard working fluid in a sample, extracting the sample by using a metaphosphoric acid-methanol solution, adding ethyl acetate in an extracting solution to be extracted, separating upper-layer ethyl acetate layer, carrying out rotary evaporation until being dried, carrying out constant-volume dissolution on the residues by using a methanol-formic acid solution, filtering through a 0.22<mu>m filter membrane, and performing qualitative and/or quantitative detection by using a liquid chromatograph-tandem mass spectrometer. The detection method for the metabolite residues of carbadox and olaquindox in the animal tissues disclosed by the invention is simple in sample treatment method and low in operation cost, the determination low limit of quinoxaline-2-carboxylic acid and 3-dimethyl quinoxaline-2-carboxylic acid is 1 mu g/kg, quinoxaline-2-carboxylic acid and 3-dimethyl quinoxaline-2-carboxylic acid are in line with a linear relationship in the range of 2 ng/mL to 200 ng/mL, the linear correlation coefficient r is more than 0.99 and the average recovery rate is ranged from 82.71% to 121.09%, and RSD is ranged from 4.07% to 9.51% (n is equal to 6), thereby indicating that the accuracy and sensitivity of the method meet detecting demands.

The invention provides a corn peptide composition for alleviating hangover and protecting liver and a preparation method thereof. The corn peptide composition comprises the following active components: corn peptide, radix puerariae, semen hoveniae and fructus lycii, and further comprises rhizoma curcumae longae. The corn peptide composition provided by the invention achieves excellent effects of alleviating hangover and protecting liver by combining the corn peptide, radix puerariae, semen hoveniae, fructus lycii and rhizoma curcumae longae at a specific ratio and playing a synergistic effect,has no toxicity and no harm, can improve ADH activity of liver tissues, promotes ethanol metabolism, increases an elimination rate of ethanol metabolites, reduces damage to tissues and cells, plays roles of alleviating hangover and promoting sobering, protects the liver, reduces dependence and use on drugs to reduce toxic and side effects of the drugs, has advantages of simple preparation methodand low production cost, and is suitable for mass consumption.

The invention discloses multifunctional health burdock tea paste. The multifunctional health burdock tea paste is prepared from burdock root, fructus arctii, green tea, mushroom and cordyceps militaris as raw materials through enzymolysis and extraction. The multifunctional health burdock tea paste has functions of burdock root, fructus arctii, green tea, mushroom and cordyceps militaris producing synergism and has abundant nutrition values and health care functions. The health care functions comprise human humoral immunity improvement, viral resistance and cancer resistance. The burdock, cordyceps militaris and mushroom extract can effectively prevent ethanol from entering blood, protect liver and accelerate an ethanol metabolism rate in vivo so that the multifunctional health burdock tea paste has effects of relieving the effect of alcohol and sobering up and is an ideal health drink.

The invention discloses an olaquindox metabolin antigen, an antibody, an enzyme-linked immunosorbent assay kit and a detection method. 4-(3-methyl-2-naphthalene carboxamide) butyrate is taken as an olaquindox metabolin hapten; the olaquindox metabolin antigen can be acquired after the hapten is coupled with carrier protein; the olaquindox metabolin antigen can be used for preparing an olaquindox metabolin specific antibody; the specific antibody can be used for preparing the enzyme-linked immunosorbent assay kit or colloidal gold test paper card used for detecting the residue of olaquindox metabolin in food. The invention also discloses the corresponding detection method of the kit. The method is simple, convenient and quick and is characterized by linear detection range within 0.2-16.2ng/mL, sensitivity of 0.54ng/mL, limit of detection of 0.32ng/mL, recovery rate within 80%-109.8%, low detection limit, high sensitivity, high specificity, high stability, low cost and suitability for mass sample detection and onsite quick detection.

The invention provides a composition for detecting ethanol metabolic capacity, and relates to the field of molecular biology detection, in particular to the field of ethanol metabolic capacity detecting. Meanwhile, the invention further provides application of the composition to detection of the ethanol metabolic capacity, a kit comprising the composition, and a method for detecting the ethanol metabolic capacity. By using the composition and the method, the detection time can be greatly shortened.

Disclosed is a Chinese medicinal preparation for accelerating alcohol metabolism, which comprises the following components (by weight percentage): hovenine seed 25-75%, giant knotweed rhizome 15-35%, cogongrass rhizome 5-25%, and amomum fruit 5-15%.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for preventing or treating side effects after drinking. The traditional Chinese medicine composition is mainly prepared from notopterygium root, radix angelicae pubescentis, poria cocos, radix saposhnikoviae, herba schizonepetae, ligusticum wallichii, platycodon grandiflorum, radix bupleuri, radix peucedani, fructus aurantii and liquorice. The traditional Chinese medicine composition disclosed by the invention can relieve oxidative stress injury of alcohol to brain tissues, so that the brain tissues are protected; the ethanol metabolism can be accelerated by increasing the activity of ADH, the activity of SOD can be increased, and the damage to the liver is reduced.

The invention relates to a method for producing sorbierite by using metal ions to process cells, comprising the following steps of adding metal ions into a system for producing sorbierite by using glucose and fructose as substrates and the zymomonas mobilis which is Gram-negative bacteria to process the cells so as to inhibit the activity of various kinds of enzyme for ethanol metabolism in the zymomonas mobilis, effectively inhibit ethanol generation, and completely convert the glucose and the fructose into sorbierite and gluconic acid. The invention has the advantages that the glucose and the fructose can be completely converted into the sorbierite and the gluconic acid by adding few metal ions into a reaction system; the processing method is simple and avoids the traditional complicated cell processing process; in addition, the products of zinc gluconate and calcium gluconate which are generated by adding some metal ions, such as Zn2+, Ca2+, and the like are objective products so that the burden of a subsequent separating and purifying process is not increased, and the production process of the sorbierite is more feasible.

The invention discloses an anti-alcohol fruit vinegar paste and a preparation method and application thereof, and belongs to the technical field of vinegar and preparation thereof. The anti-alcohol fruit vinegar paste with the anti-alcohol and liver protecting effects is prepared in the mode that corn, millet, grapes, radix puerariae, fructus phyllanthi, fructus gardeniae, shaddock peels and the like are mainly adopted as raw materials and subjected to raw material preparation, cooking and liquefaction, alcohol fermentation, acetic acid fermentation, aging and concentration. According to the prepared anti-alcohol fruit vinegar paste, a fruit vinegar brewing process is combined with anti-alcohol effect substances such as the radix puerariae, the fructus phyllanthi, the fructus gardenia andthe shaddock peels, and the anti-alcohol fruit vinegar paste is sour-sweet and tasty, unique in flavor, simple, portable and capable of promoting the ethanol metabolic effect.