33 results about "Ethanol metabolism" patented technology

The invention discloses a Lactobacillus paracasei grx06 and an application thereof in the aspects of dispelling the effects of alcohol and protecting the liver, the strain number of the Lactobacillus paracasei is grx06, the Lactobacillus paracasei is preserved in the China General Microbiological Culture Collection Center on February 18, 2025, and the preservation number is CGMCC No: 33560. The lactobacillus paracasei obtained by screening in the invention has the capability of producing ethanol dehydrogenase and acetaldehyde dehydrogenase at high yield, and is high in ethanol tolerance. In addition, the traditional Chinese medicine composition has the effects of promoting ethanol metabolism in rats and recovering liver injury. Wide application prospects are realized in the field of anti-alcohol and liver-protecting products.

The invention belongs to the technical field of medicines, and particularly relates to a plant lactobacillus for relieving acute alcoholic liver injury as well as a metagen and application of the plant lactobacillus. The strain of the plant lactobacillus L5-13 is preserved in the China Center for Type Culture Collection on August 18, 2025, and the preservation number of the strain is CCTCC NO: M 20251835. The strain and the metagen thereof can significantly improve pathological symptoms of mice with acute alcoholic liver injury, including: reducing the levels of ALT, AST, TC, TG and LDL-C in serum of the mice with acute alcoholic liver injury, and increasing the level of HDL-C; the abnormal blood lipid level of the mouse caused by acute alcohol is regulated; the activity of antioxidant enzymes in the liver is improved, and the lipid peroxidation level is reduced; the ethanol dehydrogenase activity of the liver is improved, and the ethanol metabolism capability is enhanced. Therefore, the strain and the metagen thereof have wide application prospects in prevention or treatment of acute alcoholic liver injury.

The invention relates to the technical field of pharmaceutical compositions, and particularly discloses a proteolipid composite supplement with synergistic hangover alleviating and liver protecting functions and a preparation method thereof. The health-care food comprises the following components in percentage by mass: 10-25% of a Pu'er tea extract, 15-30% of glucose syrup, 5-15% of a hovenia acerba extract, 5-15% of a radix puerariae extract, 8-20% of corn peptide, 5-15% of oyster peptide, 3-10% of earthworm peptide, 1-5% of malic acid, 2-8% of multivitamins, 1-5% of an electrolyte compound, 3-10% of prebiotic fibers and 0.5-3% of gamma-aminobutyric acid. The compound vitamins comprise B1, B2, B6, B12, folic acid and vitamin C; the electrolyte compound is a nanoscale electrolyte compound and is an organic chelate containing potassium, magnesium and zinc; the prebiotic fiber is prepared by compounding galactooligosaccharide and resistant dextrin; through compounding design of active peptide components, plant active ingredients and functional factors, the active peptide improves the activity of key enzymes in ethanol metabolism in a targeted manner, and alcohol decomposition is accelerated.

The invention relates to the technical field of health food, in particular to a selenium tea ethanol dehydrogenase activating peptide composition as well as a preparation method and application thereof. A response surface method is adopted to optimize a TSE-PP preparation process, affinity ultrafiltration and LC-MS / MS technologies are utilized to screen ADH binding peptides, and the action mechanism of the ADH binding peptides is researched through molecular docking and animal experiments. Experiments prove that the TSE-PP can promote ethanol metabolism, relieve liver cell injury, inhibit inflammatory response, relieve oxidative stress and improve liver lipid accumulation, the high-dose use effect is more remarkable, and a scientific basis is provided for application of the TSE-PP in the food with the function of dispelling the effects of alcohol and the nutrition fortified product. The invention discloses the preparation process of the TSE-PP and the anti-alcohol and liver protection effects of the TSE-PP, meanwhile, the TSE-PP has good food processing characteristics, and a theoretical basis and an experimental support are provided for production and application of the TSE-PP in functional foods.

The invention discloses a rosa roxburghii tratt fruit composite beverage with a hangover alleviating effect and a preparation method and application thereof, and belongs to the technical field of biology. The roxburgh rose beverage disclosed by the invention comprises the following raw materials in parts by weight on the basis of 100 parts by weight: 8-16 parts of roxburgh rose concentrated juice, 5-15 parts of crystalline fructose, 0.8-2.5 parts of soybean peptide powder, 0.8-1.8 parts of yeast extract and the balance of water. Experiments prove that the formula product has the advantages of moderate taste, aromatic flavor, good stability and the like, and can keep stable taste and flavor during long-term storage. Animal experiments verify that the roxburgh rose beverage has an obvious hangover alleviating effect by improving ALDH activity, accelerating ethanol metabolism and reducing blood ethanol mass concentration, can improve liver injury caused by drinking by increasing liver SOD, GSH-Px, CAT activity and GSH content, reducing MDA content, enhancing body antioxidant ability and inhibiting lipid peroxidation reaction, and has a liver protection effect.

The invention discloses a medicinal and edible composition which is characterized by comprising the following components in parts by weight: 15-20 parts of a hovenia dulcis thunb extract, 10-15 parts of a turmeric extract, 8-12 parts of a schisandra chinensis extract, 5-8 parts of a globe artichoke extract, 6-10 parts of a ganoderma lucidum spore powder extract and 0.5-1 part of a silibinin extract. According to the composite composition prepared by the invention, ethanol metabolism key enzyme (ADH / ALDH) is activated by multiple targets, acetaldehyde removal is accelerated, and a hepatocyte repairing function is supplemented, so that alcohol metabolism after drinking is accelerated, and damage of alcohol to a human body is reduced.

The invention provides application of lactobacillus brevicornu and a fermentation composition thereof in hangover alleviating and liver protection, and relates to the technical field of biological fermentation, the lactobacillus brevicornu is lactobacillus brevicornu, the strain number of the lactobacillus brevicornu is KMHD10-222, and the registration number of the lactobacillus brevicornu in the China General Microbiological Culture Collection Center is CGMCC No. 32358. Traditional Chinese medicine substrates can be fermented, and the hangover-alleviating and liver-protecting effects of fermentation products are enhanced. The content of rare ginsenoside, total polysaccharide, total flavone, total phenol, puerarin, daidzein and dihydromyricetin of the lactobacillus brevis KMHD10-222 fermentation substrate extracting solution is increased, the effects of promoting ethanol metabolism and improving the alcoholic liver disease can be improved, and the effects of dispelling the effects of alcohol and protecting the liver are achieved.

The invention relates to the technical field of medicines, in particular to a composition for relieving headache after drinking and a preparation method thereof. Comprising the following raw materials in parts by weight: 5-10 parts of curcumin composite particles, 20-30 parts of a chitosan stable fermentation liquid, 10-15 parts of a radix puerariae extract, 5-10 parts of an emblic leafflower fruit extract, 10-15 parts of Chinese wolfberry fruits, 5-10 parts of cassia seeds, 5-10 parts of smoked plums, 5-10 parts of a roselle extract, 10-15 parts of a sweetening agent and 5-8 parts of a stabilizer. Wherein the curcumin composite particles are prepared through nano-liposome embedding and tannic acid and polyethylene glycol functionalization treatment, the chitosan stable fermentation liquor is prepared by compounding chitosan and red pitaya fermentation liquor, and the composition is prepared through the processes of plant component extraction, mixing and blending, sterilization treatment and the like. Ethanol metabolism can be accelerated, and the antioxidant capacity of the liver is enhanced, so that damage of alcohol to the liver is relieved, and headache after drinking is effectively relieved.

The invention provides a polypeptide and application thereof. The polypeptide with the amino acid sequence as shown in SEQ ID NO: 1 not only can remarkably remove ABTS free radicals and DPPH free radicals and play a role in resisting oxidation, but also can activate ADH enzyme and ALDH enzyme and play a role in promoting ethanol metabolism, so that the alcoholic liver disease is effectively resisted.

The invention belongs to the technical field of medicines, and particularly relates to lactobacillus rhamnosus for relieving acute alcoholic liver injury as well as a metagen and application of the lactobacillus rhamnosus. The lactobacillus rhamnosus L11-4 strain is preserved in the China Center for Type Culture Collection on August 18, 2025, and the preservation number of the lactobacillus rhamnosus L11-4 strain is CCTCC (China Center for Type Culture Collection) NO: M 20251834. The strain and the metagen thereof can significantly improve pathological symptoms of mice with acute alcoholic liver injury, and have the effects of reducing the levels of ALT, AST, TC, TG and LDL-C in serum of the mice with acute alcoholic liver injury, increasing the level of HDL-C, and regulating abnormal blood lipid level of the mice caused by acute alcohol; the activity of antioxidant enzymes in the liver is improved, and the lipid peroxidation level is reduced; in addition, the ethanol dehydrogenase activity of the liver can be improved, and the ethanol metabolism capability is enhanced. Therefore, the strain and the metagen thereof have wide application prospects in prevention or treatment of acute alcoholic liver injury.

The invention discloses a pyruvic acid gene loop mediated saccharomyces cerevisiae ethanol metabolism redirection method. Comprising the following steps: constructing a pyruvic acid response gene loop in saccharomyces cerevisiae host bacteria to obtain modified saccharomyces cerevisiae; the growth of saccharomyces cerevisiae cells, the synthesis of by-product ethanol and the synthesis of products are dynamically regulated and modified by controlling glucose feeding. A low-concentration glucose environment is maintained in the early stage of growth, so that cell growth and ethanol metabolism related genes are normally expressed, and cell growth is recovered; high-concentration glucose is supplemented in the later stage of fermentation, expression of cell growth related ethanol metabolism genes is inhibited, the ethanol synthesis flux is reduced, sufficient pyruvic acid precursors are provided for product synthesis, and the product synthesis flux is maximized. According to the method, the yield of the byproduct ethanol is reduced, the production efficiency of the product is improved, and meanwhile, the fermentation period is shortened.

The invention provides pentapeptide and application thereof in preparation of a medicine for preventing and / or treating alcoholic liver injury. The pentapeptide with the amino acid sequence shown as SEQ ID NO: 1 not only can remarkably remove DPPH free radicals and ABTS free radicals and play an anti-oxidation role, but also can activate ADH enzyme and ALDH enzyme and play a role in promoting ethanol metabolism, so that the alcoholic liver injury is effectively prevented and / or treated, and the pentapeptide is very suitable for preparing the medicine for preventing and / or treating the alcoholic liver injury.

The present invention provides a kombucha fortified lactic acid bacteria beverage and a preparation method thereof, and the kombucha fortified lactic acid bacteria beverage comprises the following raw materials by weight: 3%-6% of white granulated sugar, 0.4%-1.0% of dry tea leaves, 3%-7% of microorganisms, 0.1%-0.3% of a stabilizer, 0.1%-0.3% of citric acid, and the balance purified water. The kombucha beverage added with the lactic acid bacteria can generate more healthy organic acids, improves the flavor of the beverage, is softer in smell, reduces the irritant sour taste, and improves the probiotic efficacy of the beverage at the same time. In the fermentation process, the white granulated sugar is a main carbon source and energy, the kombucha utilizes a white granulated sugar metabolism principle that yeast hydrolyzes the white granulated sugar into glucose and fructose through invertase, ethanol is generated through glycolysis, and metabolites are used as substrates for producing organic acids through metabolism of acetic bacteria and lactic acid bacteria; and meanwhile, the yeast can synthesize volatile acidic esters and fruity aromatic compounds which are very important for developing the aroma of black tea, so that the flavor and quality of the kombucha beverage are improved.

The invention discloses a brain tissue ethanol metabolism rate evaluation method. The method comprises the following steps: converting the ethanol content of a brain tissue by using serum; and calculating the area (AUC) under the drug-time curve of the brain tissues of the mice in each experimental group, and grading the metabolic capability of the wine body after drinking. According to the present invention, the influence of the alcohol beverage on the ethanol metabolism rate is represented by the ethanol content change of the brain tissue, such that the accumulation degree of the ethanol in the brain can be visually evaluated, the data reference is provided for the research of the relationship between the ethanol concentration and the after-drinking comfort level of the body, and the use of the experimental animal can be reduced through the method;

The invention provides an application of an active peptide in preparation of a product for preventing and treating ALD (Atomic Lymphosis Disease). The amino acid sequence of the active peptide is as shown in SEQ ID NO: 1, and the active peptide can activate ADH enzyme and ALDH enzyme to play a role in promoting ethanol metabolism, can also remarkably remove DPPH free radicals and ABTS free radicals to play an anti-oxidation role so as to effectively prevent and / or treat ALD, and is very suitable for preparing products for preventing and treating ALD.

The invention discloses application of elaeagnus angustifolia polysaccharide in preparation of a product for preventing and / or treating alcoholic liver injury, and belongs to the technical field of polysaccharide. The invention provides an application of elaeagnus angustifolia polysaccharide in preparation of a product for preventing and / or treating alcoholic liver injury, which verifies the prevention / treatment effect of the elaeagnus angustifolia polysaccharide on the alcoholic liver injury by adopting a mouse alcoholic liver injury model through pathological histological observation, liver function indexes, oxidative stress indexes, inflammatory factors and ethanol metabolism related enzymes. Results show that the elaeagnus angustifolia polysaccharide can improve the level of enzymes related to alcohol metabolism, improve redox imbalance caused by alcoholic liver injury, enhance the antioxidant ability of a body and relieve inflammatory injury of liver tissues by regulating and controlling an NF-kappa B pathway and regulating oxidative stress related indexes, inhibit overexpression of inflammatory factors, improve the level of enzymes related to alcohol metabolism, and can be used for preventing and treating liver injury. Therefore, the toxic effect of alcohol on the liver is remarkably reduced, and the effect of preventing and / or treating the alcoholic liver injury is achieved.

The invention provides cordyceps militaris corn peptide fermentation liquor as well as a preparation method and application thereof, and belongs to the technical field of corn peptide deep processing. The preparation method of the cordyceps militaris corn peptide fermentation liquor comprises the following steps: firstly, performing enzymolysis on a corn peptide powder solution by adopting alpha-amylase, and then adding saccharifying enzyme for performing enzymolysis to obtain enzymatic hydrolysate; glucose is added into the enzymatic hydrolysate, the pH is adjusted to be 5-7, then cordyceps militaris C.LSG-1 is added for fermentation, and cordyceps militaris corn peptide fermentation liquor is obtained. The corn peptide is subjected to enzymolysis treatment, so that the utilization efficiency of the raw materials in a fermentation system is improved, and subsequent cordyceps militaris fermentation can be smoothly carried out; cordyceps militaris is utilized to ferment corn peptide, so that the flavor is improved, and the functional characteristics of the corn peptide can be enhanced and improved. An in-vivo model verifies that the cordyceps militaris corn peptide fermentation liquor prepared by the preparation method disclosed by the invention has better functions of promoting ethanol metabolism and preventing and treating alcoholic liver injury.

The invention discloses a freeze-drying reagent for human body ethanol metabolism gene detection and preparation and detection methods, the reagent is obtained by freeze-drying a reaction system, and the reaction system is a q-PCR detection reaction tube containing a reagent A and a reagent B. Compared with the existing liquid reagent, the freeze-drying reagent provided by the invention has the characteristics of supporting normal-temperature storage, being convenient to transport, being capable of getting rid of the problem of liquid cold chain transportation, being stored for more than 2 years, and having the positive effects of being not limited by a detection environment, getting rid of the problem of dependence on laboratory conditions, and being free from operation and interpretation of professionals; the method is convenient for non-professionals to operate, the detection result is not influenced, and the use requirement of the freeze-drying reagent is met, so that the use scene of the freeze-drying reagent can be expanded, and the method has great market competitiveness.

The present invention discloses the use of a plant polysaccharide in the preparation of a product for hangover relief and liver protection. The present invention proposes for the first time that frangipani polysaccharide can be effectively used for hangover relief and liver protection, including enhancing the activity of ADH and ALDH, key enzymes in the ethanol metabolic pathway, accelerating the rate of ethanol metabolism in the body, and reducing liver damage caused by drunkenness, having a protective effect on the body. At the same time, it can reduce the drunkenness rate, shorten the drunkenness time, significantly reduce the body's ALT, AST, TC, TG and LDL-C levels, increase the HDL-C level, significantly increase the body's liver CAT, SOD, and GSH activities, reduce the liver's MDA level, and significantly improve the body's liver fat accumulation, providing a new direction for the preparation of hangover relief and liver protection products.

The invention relates to an anti-alcoholism composition, a liquid preparation with an anti-alcoholism effect and a preparation method and application of the liquid preparation, and belongs to the technical field of physiology. The anti-alcohol composition comprises an NAD (nicotinamide adenine dinucleotide) precursor substance and amino acid, and the NAD precursor substance is one or more of nicotinamide, nicotinamide ribose, nicotinamide mononucleotide or nicotinic acid; and the amino acid is one or more of L-glutamine, L-serine or L-lysine. The special combination of the NAD precursor substance and the amino acid can synergistically accelerate ethanol metabolism, and the alcohol effect dispelling liquid preparation prepared on the basis of the alcohol effect dispelling liquid preparation is flexible in use time, controllable in component and outstanding in alcohol effect dispelling effect, and has very wide application prospects.

The invention provides a plant polypeptide beverage with functions of dispelling effects of alcohol and protecting liver as well as a preparation method and application of the plant polypeptide beverage. By compounding the corn oligopeptide powder, the wheat oligopeptide powder, the concentrated apple clear juice, the radix puerariae powder, the hovenia dulcis thunb powder and other natural components, a synergistic interaction system is constructed; the corn / wheat oligopeptide provides amino acid complementation and activates ADH and ALDH of the liver; the radix puerariae powder and the semen hoveniae powder inhibit acetaldehyde accumulation; the concentrated apple clear juice, the acerola cherry powder and the wolfberry powder enhance the antioxidant activity. During preparation, high-pressure homogenization and UHT instantaneous sterilization processes are adopted, and polydextrose is combined to form a stable colloid. Experiments show that the beverage can improve the ethanol metabolism rate and reduce the levels of ALT and AST in serum. In addition, the levels of SOD, GSH-Px and GSH in the liver are increased, the antioxidant capacity of the liver is improved, damage of ethyl alcohol to the liver is relieved, and the beverage has taste palatability and component stability and is suitable for dispelling the effects of alcohol and preventing alcoholic liver injury.

The invention relates to the technical field of health food, and discloses a composition for sobering up, assisting in lowering lipid and improving intestinal tracts and a preparation method, the composition comprises the following components in parts by weight: 40-60 parts of rubus stems and leaves, 3-6 parts of oldenlandia diffusa, 1-3 parts of sculellaria barbata, 0.5-2 parts of balsam pear, 0.5-2 parts of radix bupleuri, 0.5-2 parts of pericarpium citri reticulatae, 0.5-2 parts of elecampane, 0.5-2 parts of raw hawthorn and 0.5-2 parts of semen plantaginis. The preparation method comprises the steps of raw material crushing, enzymolysis extraction, alcohol extraction, water extraction, spray drying and the like. Experimental verification shows that the composition has multiple effects of prolonging the drunkenness latency period, accelerating ethanol metabolism, reducing serum transaminase, regulating blood lipid indexes, improving intestinal peristalsis and flora structures and the like, and acute toxicity tests prove that the composition is high in edible safety. The composition disclosed by the invention can be used for preparing health-care foods with the effects of dispelling the effects of alcohol, assisting in lowering lipid and improving intestinal functions.

The present invention relates to the field of medical technology, and in particular to a composition for relieving headaches caused by alcohol consumption and a preparation method thereof. The composition comprises the following raw materials in parts by weight: 5-10 parts of curcumin composite particles, 20-30 parts of chitosan stabilized fermentation broth, 10-15 parts of kudzu root extract, 5-10 parts of emblica fruit extract, 10-15 parts of wolfberry fruit, 5-10 parts of cassia seed, 5-10 parts of black plum, 5-10 parts of roselle extract, 10-15 parts of sweetener, and 5-8 parts of stabilizer. The curcumin composite particles are prepared by nanoliposome embedding and tannic acid and polyethylene glycol functionalization treatment, the chitosan stabilized fermentation broth is prepared by combining chitosan and red pitaya fermentation broth, and the composition is prepared by processes such as plant component extraction, mixing and blending, and sterilization treatment. The composition can accelerate ethanol metabolism, enhance liver antioxidant capacity, thereby reducing liver damage caused by alcohol, and effectively relieve headaches caused by alcohol consumption.

The invention belongs to the technical field of biological medicine, and particularly discloses a water-soluble pachymaran composition and application thereof in alcohol metabolism regulation. The mass concentration ratio of the water-soluble pachymaran to the kudzuvine root extract is 1: (1-2), the substitution degree of the water-soluble pachymaran is 0.6-0.7, the kudzuvine root extract is an ethanol extract of kudzuvine root powder, and the water-soluble pachymaran and the kudzuvine root extract play a synergistic role in regulating alcohol metabolism. On one hand, by promoting the functions of enzymes related to ethanol metabolism, the ethanol metabolism and removal efficiency is improved; on the other hand, the adverse effects of alcohol and metabolites thereof on the body are reduced by relieving inflammation and stress response accompanied by alcohol metabolism, so that a good overall effect is shown in the aspects of detoxification and alcohol metabolism regulation. The water-soluble pachymaran composition provided by the invention has the effect of improving the physiological status after alcohol intake, and has a wide application prospect in the fields of hangover alleviating and alcoholic injury protection.

The invention relates to the technical field of health-care food, and particularly discloses a fermented beverage for relieving discomfort after drinking and a preparation method and application thereof.The fermented beverage comprises pomegranate seed extract and medicated leaven prepared through fermentation of composite yeast seeds containing aspergillus oryzae and saccharomyces cerevisiae, the pomegranate seed extract is pomegranate polyphenol extract, and in the pomegranate polyphenol extract, aspergillus oryzae and saccharomyces cerevisiae are added into the fermented beverage; the total polyphenol content is not lower than 40%, the resveratrol content is not lower than 1.5%, the fermented beverage further comprises malt, a malt extract, hovenia dulcis thunb, edible yeast, a radix puerariae extract and a radix puerariae flower extract, and the prepared fermented beverage is applied to food or health care products for accelerating ethanol metabolism in blood and relieving dizziness, nausea or weakness and discomfort after drinking. The product is stable in efficacy and suitable for preparing food or health care products with the hangover alleviating function.

The application discloses application of plant starch in preparation of a product for dispelling alcohol and protecting liver. The application first proposes that the Xanthosoma sagittifolium starch can be effectively used for dispelling alcohol and protecting liver, including enhancing the activity of key enzymes ADH and ALDH in an ethanol metabolic path, accelerating the ethanol metabolic speed in the body, reducing the liver damage caused by drunkenness, having the protection effect on the organism, simultaneously, being capable of reducing the drunken rate, shortening the drunken time, significantly reducing the ALT, AST, TC, TG and LDL-C content of the organism, increasing the HDL-C content, significantly improving the CAT, SOD and GSH activity of the liver of the organism, reducing the MDA level of the liver, and significantly improving the liver fat accumulation of the organism, and providing a new direction for preparation of the product for dispelling alcohol and protecting liver.

The invention discloses an application of plant polysaccharide in preparation of a product for dispelling effects of alcohol and protecting liver. The jasminum grandiflorum polysaccharide can be effectively used for dispelling the effects of alcohol and protecting the liver for the first time, the activity of key enzymes ADH and ALDH in an ethanol metabolism path is enhanced, the in-vivo ethanol metabolism speed is increased, liver damage caused by drunkenness is relieved, the jasminum grandiflorum polysaccharide has the body protection effect, meanwhile, the drunkenness rate can be reduced, the drunkenness time can be shortened, the content of ALT, AST, TC, TG and LDL-C in the body can be remarkably reduced, and the effect of preventing and treating the drunkenness is achieved. According to the invention, the content of HDL-C is increased, the activity of CAT, SOD and GSH in the liver of the organism is remarkably improved, the MDA level of the liver is reduced, the fat accumulation condition of the liver of the organism is remarkably improved, and a new direction is provided for preparation of products for dispelling the effects of alcohol and protecting the liver.

The present invention belongs to the field of medical technology, and specifically relates to the use of Huoxiang Anwei Powder in sobering up, preparing products for preventing, treating and / or treating liver and stomach damage caused by alcohol. The Huoxiang Anwei Powder of the present invention has a significant protective effect on alcohol-induced acute drunkenness and related liver and stomach damage, and this protective effect is related to the fact that Huoxiang Anwei Powder enhances ethanol metabolism, improves antioxidant capacity, reduces lipid peroxidation and inhibits inflammatory response. The formula of Huoxiang Anwei Powder of the present invention has high dose efficiency and synergistic effect, works on multiple levels, and provides more comprehensive protection.

The inactivated lactic acid bacteria and the metagen thereof disclosed by the invention can be used for preparing a product for inhibiting helicobacter pylori. Besides, the pediococcus acidilactici Lab2 metagen and the acetobacter aceti AS1.41 metagen are compounded, it is found that compounding of the pediococcus acidilactici Lab2 metagen and the acetobacter aceti AS1.41 metagen is remarkably higher than that of single Lab2 metagen or acetobacter aceti metagen, and in the ethanol metabolism level, the compound metagen remarkably improves ADH and ALDH activity of the mouse liver. Secondly, on the oxidative stress regulation level, the SOD, CAT activity and GSH content of the mouse liver are remarkably improved by compounding the metabiotics, and meanwhile, the MDA level is reduced. In the aspects of liver function protection and lipid metabolism regulation, the compound metabiotics can significantly reduce the activity of ALT and AST in serum, and can significantly reduce the level of TG and TC in serum at the same time. The liver tissue pathological section further proves that the composite metagen can obviously relieve hepatocyte edema and microvesicle fatty degeneration, recover normal structural arrangement of the liver tissue and realize comprehensive protection on the alcoholic acute liver injury.