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A rapid detection kit for bacterial vaginosis and its preparation method and application

A technology for detecting kits and vaginosis, applied in the field of medical testing, to achieve the effects of improving sensitivity, improving detection efficiency, and shortening color development time

Active Publication Date: 2019-08-23
GUANGZHOU JIANGYUAN MEDICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the defects of the bacterial vaginosis detection kit in the prior art, the purpose of the present invention is to provide a rapid detection kit for bacterial vaginosis. This technical solution is the inventor's further research on existing products. to address deficiencies in existing products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. A rapid detection kit for bacterial vaginosis The rapid detection kit for bacterial vaginosis consists of reagent R1 and reagent R2 with a volume ratio of 10:3:

[0033] The reagent R1 consists of the following components and their concentrations:

[0034] 5-bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid salt 0.2g / L, polyethylene glycol 2000.1g / L, and the balance is phosphate buffer; the phosphate buffer The pH value is 6.5.

[0035] The reagent R2 consists of the following components and their concentrations:

[0036] 0.3 mg / L of 4-aminoantipyrine, and the balance is phosphate buffer; the pH value of the phosphate buffer is 7.5.

[0037] Preparation:

[0038]S1 Add polyethylene glycol 200 to the phosphate buffer and stir evenly, let it stand for 25 minutes, then add 5-bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid salt, stir evenly to obtain the reagent R1;

[0039] S2 Add 4-aminoantipyrine to the phosphate buffer solution, stir evenly to ob...

Embodiment 2

[0040] Example 2, a rapid detection kit for bacterial vaginosis The rapid detection kit for bacterial vaginosis consists of reagent R1 and reagent R2 with a volume ratio of 10:3:

[0041] The reagent R1 consists of the following components and their concentrations:

[0042] 5-bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid salt 0.25g / L, polyethylene glycol 2000.15g / L, and the balance is phosphate buffer; the phosphate buffer The pH value is 6.6.

[0043] The reagent R2 consists of the following components and their concentrations:

[0044] 0.35 mg / L of 4-aminoantipyrine, and the balance is phosphate buffer; the pH value of the phosphate buffer is 7.8.

[0045] The preparation method is similar to Example 1.

Embodiment 3

[0046] Example 3, a rapid detection kit for bacterial vaginosis The rapid detection kit for bacterial vaginosis consists of reagent R1 and reagent R2 with a volume ratio of 10:3:

[0047] The reagent R1 consists of the following components and their concentrations:

[0048] 5-bromo-4-chloro-3-indolyl α-D-N-acetylneuraminic acid salt 0.3g / L, polyethylene glycol 2000.2g / L, and the balance is phosphate buffer; the phosphate buffer The pH value is 6.8.

[0049] The reagent R2 consists of the following components and their concentrations:

[0050] 0.4mg / L of 4-aminoantipyrine, and the balance is phosphate buffer; the pH value of the phosphate buffer is 8.0.

[0051] The preparation method is similar to Example 1.

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PUM

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Abstract

The invention belongs to the technical field of medical examination, and particularly relates to a rapid detection kit for bacterial vaginosis, a preparation method and application thereof. The kit comprises a reagent R1 and a reagent R2, and the reagent R1 is composed of 5-bromine-4-chlorine-3-Indolyl alpha-D-N-acetylneuramidate, polyethylene glycol 200 and phosphate buffer solution; and the reagent R2 is composed of 4-amino antipyrine and the phosphate buffer solution. The rapid detection kit for the bacterial vaginosis has the advantages of high determination sensitivity, high accuracy of detection results, good repeatability and high stability, and is an ideal rapid detection kit for the bacterial vaginosis, and the popularization and application of the kit are facilitated.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a rapid detection kit for bacterial vaginosis and its preparation method and application. Background technique [0002] Bacterial vaginosis (Bacterial vaginosis, BV) is a common vaginal infectious disease in women of childbearing age. It is due to the disturbance of the ecological balance of normal vaginal flora, the dominant flora Lactobacillus decreases or disappears, while Gardnerella vaginalis, Mixed infection without inflammatory changes of vaginal mucosa caused by overgrowth of anaerobic bacteria, Campylovibrio and other microorganisms. BV can lead to organizational choriovitis, amniotic fluid infection, endometritis after laparotomy and other adverse pregnancy and pregnancy complications, and is associated with common gynecological diseases such as salpingitis, pelvic inflammatory disease, ectopic pregnancy, infertility, urinary system infection, Posto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/40C12Q1/04
Inventor 李小梅骆伟明李飞李高峰申令
Owner GUANGZHOU JIANGYUAN MEDICAL SCI & TECH CO LTD
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