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Immunoassay method, system for identifying immunoassay and kit

A technology of immunoassay and kit, which is applied in the field of kits for detection of thyrotropin, which can solve the problems that the signal cannot be continuously increased and the detection range is limited.

Active Publication Date: 2019-03-15
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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  • Abstract
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Problems solved by technology

[0009] On the other hand, because of the high dose-hook effect, when the sample concentration increases to a certain value, the signal cannot continue to increase, which limits the detection range

Method used

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  • Immunoassay method, system for identifying immunoassay and kit
  • Immunoassay method, system for identifying immunoassay and kit
  • Immunoassay method, system for identifying immunoassay and kit

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Embodiment 1

[0103] Embodiment 1: Conventional method and method of the present invention detect thyrotropin (TSH) sample respectively

[0104] Thyrotropin detection kit (light-induced chemiluminescence method) produced by Boyang Biotechnology (Shanghai) Co., Ltd. was used to detect the content of thyrotropin (purchased from Fitzgerald, Catalog No: 30R-AT009) in the sample.

[0105] The high-concentration TSH antigen is serially diluted, and the signal values ​​of samples containing different concentrations of TSH are measured by conventional detection methods and the detection method of the present invention.

[0106] Routine detection method: TSH samples with gradient dilution, reagent 1 (luminescent particles coated with TSH primary antibody) and reagent 2 (biotin-labeled TSH secondary antibody) were added to the cuvette, incubated at 37°C for 15 minutes, and then added LiCA universal solution (streptavidin-labeled photosensitive particles), incubated at 37° C. for 10 min, and read the ...

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Abstract

The invention relates to an immunoassay method of thyroid stimulating hormone in the technical field of light activated chemiluminiscence. The immunoassay method of the thyroid stimulating hormone comprises the following steps: (1) carrying out chemiluminiscence immunoreaction on a thyroid stimulating hormone containing sample to be detected, activating and recording a first reading and a second reading of chemiluminiscence, and recording a difference increasing rate between the second reading and the first reading as A; (2) correspondingly making a first standard curve and a second standard curve according to the first reading of a series of known thyroid stimulating hormone containing standard substance and an increasing rate A' between the two readings; and (3) correspondingly comparingthe first reading of the thyroid stimulating hormone containing sample to be detected and the increasing rate A between the two readings with the first standard curve and the second standard curve todetermine the concentration of the sample. The invention further relates to a system for identifying the thyroid stimulating hormone immunoassay and a kit used for detecting the thyroid stimulating hormone.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of November 22, 2016, an application number of 201611034237.7, and an invention title of "immunoassay method, system and kit for identifying immunoassay". technical field [0002] The invention relates to the technical field of light-induced chemiluminescence, in particular to a thyrotropin immunoassay method, a system for identifying thyrotropin immunoassay and a test kit for detecting thyrotropin. Background technique [0003] Immunological detection is based on the principle of antigen-antibody specific reaction. Because it can use isotopes, enzymes, chemiluminescent substances, etc. to display or amplify the signal of the measured substance, it is often used to detect trace organisms such as proteins and hormones. active substance. [0004] Chemiluminescent immunoassay is a non-radioactive immunoassay technology that has developed rapidly in recent years. Its prin...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/78G01N33/58G01N21/76
CPCG01N21/636G01N21/76G01N33/53G01N33/54313G01N33/5761G01N33/58G01N33/6854G01N33/6893G01N33/78G01N2021/637G01N2333/72
Inventor 杨阳赵卫国张向辉
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD