Application of annexin V

A technology of annexin and diabetes, which is applied in the field of biomedicine, can solve unclear problems, achieve the effect of lowering blood sugar levels, improving physiological indicators and metabolic status

Active Publication Date: 2019-03-29
JIANGSU TARGET BIOMEDICINE RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of annexin V
  • Application of annexin V
  • Application of annexin V

Examples

Experimental program
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Embodiment 1

[0040] (1) Expression and Preparation of Annexin V

[0041] Recombinant Annexin V expressed in BL21(DE3) Escherichia coli, purified recombinant Annexin V was obtained by affinity chromatography and ion exchange chromatography; all prepared solutions were made of sterile endotoxin-free water ; Use BCA kit to measure protein concentration; after purification, analyze the purity of protein product by SDS-PAGE electrophoresis, more than 98%. Then it is either an aqueous solution in the form of a solution, or a freeze-dried powder with good reconstitution properties. Subsequent studies proved that the various activities of recombinant annexin in different preparations were comparable.

[0042] (2) Induce experimental hyperglycemia model

[0043] Fifty 8-9 week old male B6 mice were randomly divided into five groups:

[0044] Group I - control group: with phosphate buffered saline (PBS);

[0045] Group II - Model Group: STZ;

[0046] Group III - low dose group: 1.25 mg / kg / day a...

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Abstract

The invention belongs to the technical field of biology, particularly discloses an application of annexin V to the preparation of a medicine for delaying diabetes progression, and specifically comprises an application of the annexin V to the preparation of a medicine for the in preparing a medicament for improving physiological index and metabolism of diabetes, improving insulin signal pathway ofdiabetic model, improving pathological changes of diabetes liver, pancreas and kidney, improving diabetes serum creatinine and urea nitrogen levels, preventing diabetic renal complications or renal function damage and protecting insulinoma cells from apoptosis caused by diabetes inducer streptozotocin.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an application of annexin V in the preparation of a drug for delaying the progress of diabetes. Background technique [0002] Diabetes is a chronic endocrine and metabolic disease that seriously threatens human health. In recent years, the incidence of diabetes has gradually increased, which is characterized by persistent blood sugar elevation. If hyperglycemia is not effectively controlled, it will lead to infection, damage to multiple organs and tissues, lower immune function, and great threat to health. Streptozotocin (STZ)-induced hyperglycemia animal model is currently a commonly used diabetes research model. [0003] Annexin V (Annexin V) is a member of the Annexin family of phospholipid-binding proteins. Annexin family proteins to depend on Ca 2+ combined with phospholipids. Phosphatidylserine (PS) and lysophosphatidylcholine (LPC) are involved in the r...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P3/10
CPCA61K38/17A61P3/10
Inventor 华子春林艳
Owner JIANGSU TARGET BIOMEDICINE RES INST
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