A method for identification and evaluation of rice panicle blast resistance
A technology of panicle blast and rice, which is applied in the field of rice disease identification, can solve the problems of inaccurate resistance identification, easy elimination of good varieties, etc., and achieve the effect of overcoming severe disease
Active Publication Date: 2021-08-27
HUNAN HYBRID RICE RES CENT
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Problems solved by technology
[0005] The invention provides a method for identifying and evaluating the resistance of rice blast blast to solve the technical problem that the existing rice blast resistance identification is inaccurate and it is easy to eliminate good varieties
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[0035] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
[0036] In order to be closer to the natural identification environment, and more efficient and true to respond to variety resistance, the present invention responds to the resistance identification method of rice panicle blast by inoculating induced varieties and mainly includes the following steps: (1) collection, isolation and preservation of bacterial strains; (2) Selection and cultivation of bacterial strains; (3) Selection of induced varieties; (4) Selection of natural identification disease nurseries; (5) Sowing, seedling raising and transplanting of rice varieties to be identified and induced varieties; (6) Strain inoculation ; (7) Disease investigation and evaluati...
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A method for identification and evaluation of rice panicle blast resistance of the present invention uses the induced species with staggered growth periods to inoculate multiple monospore strains alone, and cooperates with the existing field strains in the disease garden, so that the richness of the strains in the identified field blocks can be improved. , so that the duration of spore release is increased, so that the spore concentration of rice varieties at the growth stage is sufficient and abundant when the panicle neck is onset during the entire heading maturity stage, and rice varieties with different growth stages can be sown and identified at the same time. Simultaneously, the present invention overcomes the shortcomings such as the direct injection of variety method with excessive disease incidence and the unstable disease effect caused by the natural onset method, and can be effectively used as a means for identification of rice variety panicle blast resistance, screening for rice parental resistance, rice resistance Provide reliable solutions for breeding and rice resistance resource screening.
Description
technical field [0001] The invention relates to a rice disease identification method, more specifically, to a method for identification and evaluation of rice panicle blast resistance. Background technique [0002] As the main food crop, rice suffers huge losses due to pests and diseases every year. Rice blast is a fungal disease caused by the rice blast fungus Magnaporthe oryzae (clonal generation: Pyricularia oryzae), which is widely distributed all over the world and seriously endangers the production safety of rice, wheat and other food crops. With the gradual increase of population and the gradual reduction of arable land, the increase of grain production is facing enormous pressure. Rice blast is an important bottleneck that threatens the safety of rice production. The occurrence of rice blast often leads to a substantial reduction in rice yield. In 1980, more than 50% of the yield loss in Zhejiang Province was caused by rice blast; in 1981, early rice blast in sout...
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IPC IPC(8): C12Q1/18C12Q1/04C12R1/645
CPCC12Q1/04C12Q1/18
Inventor 彭志荣邓华凤邢俊杰石银丰
Owner HUNAN HYBRID RICE RES CENT