Helicobacter pylori specific molecular marker and detection kit

Abstract

The invention relates to a helicobacter pylori specific molecular marker and a detection kit thereof. The kit is used for performing specific detection on helicobacter pylori in samples of gastric mucosa, gastric juice, excrement and throat swab according to PCR-reverse dot blot. A target gene at which the helicobacter pylori specific molecular marker aims is a urease ureH gene. With the adoptionof the kit, sensitivity of clinical examination of helicobacter pylori is improved greatly, and furthermore, helicobacter pylori infected persons, especially old people and children can be subjected to noninvasive screening, so that early detection and early treatment can be realized; besides, for detection of other helicobacter pylori genes such as cag and vec and other drug resistance genes, thekit can be taken as positive reference and used for monitoring all processes such as genome extraction, PCR and hybridization of other genes.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a gastric Helicobacter pylori-specific molecular marker and a detection kit, in particular to a kit for detecting gastric Helicobacter pylori by PCR-reverse dot hybridization. Background technique [0002] my country is the hardest-hit area for Helicobacter pylori (Hp) infection, with an infection rate as high as 40%-60%. The common meal system and the eating habits and poor sanitation environment in a few underdeveloped areas make the infection rate of children reach 30%-40%. Most adult H. pylori infections are acquired during childhood, mostly in early childhood, and are generally difficult to clear spontaneously after infection, leading to lifelong infection. Therefore, the screening and treatment research of Hp is very important to reduce the infection rate of Hp and improve the cure rate of Hp. [0003] At present, there are mainly the following methods for H...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a specific molecular marker and detection kit for gastric Helicobacter pylori, and realize the specific detection of Helicobacter pylori according to the principle of PCR-reverse dot hybridization method, so as to solve the problem of detection of Helicobacter pylori in the prior art. The invasive detection of Helicobacter is not suitable for children and the elderly, and the sensitivity is not enough. The molecular marker and kit of the present invention can also be used as a positive reference in the detection of other Helicobacter pylori genes such as cag, vec and other drug-resistant genes. In order to monitor all the processes of other genes in genome extraction, PCR and hybridization, and promote further in-depth research on Helicobacter pylori

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