Biologic ink with uniformity-distribution cells based on 3D printing and assembly method thereof

A bio-ink and 3D printing technology, applied in medical science, prosthesis, additive processing, etc., can solve the problem of uneven cell distribution, achieve the effects of slowing down cell deposition, good application prospects, and maintaining biocompatibility

Active Publication Date: 2019-04-05
深圳大洲医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is that during the 3D printing pro

Method used

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  • Biologic ink with uniformity-distribution cells based on 3D printing and assembly method thereof
  • Biologic ink with uniformity-distribution cells based on 3D printing and assembly method thereof
  • Biologic ink with uniformity-distribution cells based on 3D printing and assembly method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The bioink body A of the embodiment of the present invention is made up of the following mass fraction components:

[0049]

[0050]The preparation method of the bio-ink body A of the embodiment of the present invention is as follows: dissolve the methacrylic anhydride gelatin in the HEPES equilibrium buffer solution according to the above ratio, add photoinitiator 2959, and stir at 50°C to obtain a uniformly mixed solution. Filter and sterilize with PES membrane filter, then cool down to 37°C, finally add silk protein and fetal bovine serum, and mix evenly by ultrasonic.

[0051] Will 1╳10 6 A bioactive cell was added to the prepared bio-ink body A and mixed evenly to obtain bio-ink A.

Embodiment 2

[0053] The bioink body B of the embodiment of the present invention is made up of the following mass fraction components:

[0054]

[0055] The preparation method of the bio-ink body B of the embodiment of the present invention is the same as that of embodiment 1.

[0056] Will 1╳10 6 A bioactive cell was added to the prepared bio-ink body B and mixed evenly to obtain bio-ink B.

Embodiment 3

[0058] The bioink body C of the embodiment of the present invention is made up of the following mass fraction components:

[0059]

[0060] The preparation method of the bio-ink body C of the embodiment of the present invention is the same as that of the embodiment 1.

[0061] Will 1╳10 6 A bioactive cell is added to the prepared bio-ink body C and mixed evenly to obtain bio-ink C.

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Abstract

The invention discloses biologic ink with uniformity-distribution cells based on 3D printing. The biologic ink with the uniformity-distribution cell comprises a biologic ink body and biologic active cells suspended in the biologic ink body, wherein the biologic ink body is prepared from the following ingredients: methacrylic anhydride gelatin, fibroin microspheres, fetal calf serum, a photo-crosslinking initiator and a balance buffer solution. The biologic ink adopting the technical scheme has the advantages that the viscosity of the biologic ink is improved by adding the fibroin microspheres;the sinking of cells at each layer surface of the biologic ink is retarded by increasing the buoyancy force received by the biologic active cells in the biologic ink; the cell deposition phenomenon in a biologic 3D printing process is relieved; all ingredients are water-soluble and have good biocompatibility; no cytotoxicity exists; biologic active ingredients are contained; the biodegradation can be realized; good application prospects are realized in complicated and refined biologic printing and composite cell biologic printing.

Description

technical field [0001] The invention relates to a biological ink, in particular to a 3D printing-based biological ink with uniform distribution of cells and an assembly method thereof. Background technique [0002] With the advancement of biomaterial technology, 3D bioprinting, as an emerging technology, has high application prospects in the field of tissue engineering because of its good biocompatibility and the ability to achieve bioregenerative repair. [0003] Among them, bio-ink, bio-printing technology and target biological cells have been extensively and in-depth researched as the three major elements of bio-3D printing. Due to the diversity, complexity and precision of human tissue structure, the 3D bioprinting process of its bionic structure takes a long time, and the target cells in the bio-ink will fall and deposit at the bottom of the barrel due to the action of gravity, resulting in the printed tissue The cells are unevenly distributed in the structure. Conte...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/38B33Y70/00
CPCA61L27/222A61L27/227A61L27/3804B33Y70/00
Inventor 朱铠孙晓宁陈楠张炜佳程蕾蕾王春生
Owner 深圳大洲医学科技有限公司
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