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Application of circular RNA as diagnostic biomarker and therapeutic target for gastric cancer and colorectal cancer

A biomarker, colorectal cancer technology, applied in the direction of biochemical equipment and methods, microbial determination/examination, etc., to achieve the effect of good ability to diagnose gastric cancer and colorectal cancer

Active Publication Date: 2019-04-12
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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Problems solved by technology

However, the specific mechanism of action of most circRNAs in cancer has not yet been elucidated, so in-depth research on the mechanism of action of circRNAs in gastric cancer and colorectal cancer has potentially great significance for the diagnosis and treatment of gastric cancer and colorectal cancer

Method used

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  • Application of circular RNA as diagnostic biomarker and therapeutic target for gastric cancer and colorectal cancer
  • Application of circular RNA as diagnostic biomarker and therapeutic target for gastric cancer and colorectal cancer
  • Application of circular RNA as diagnostic biomarker and therapeutic target for gastric cancer and colorectal cancer

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Embodiment

[0020] 1. Experimental materials and methods:

[0021] Clinical samples: Tumor tissues and adjacent normal mucosal tissues were collected from 30 patients with gastric cancer and 60 patients with colorectal cancer who received surgical treatment in Beijing Chaoyang Hospital Affiliated to Capital Medical University during 2016-2018. Tissue samples were quickly frozen in liquid nitrogen and stored in a -80°C refrigerator. The preoperative blood of the above 30 patients with gastric cancer and 60 patients with colorectal cancer was collected. In addition, the blood of 45 age- and sex-matched healthy subjects was collected as a control. Plasma was obtained by centrifugation and stored in a -80°C freezer.

[0022] Cell lines and cell culture: human gastric cancer cell line HGC-27 and colorectal cancer cell line SW480 were purchased from American Type Culture Collection (ATCC). Cells were cultured in DMEM (Invitrogen, Carlsbad, CA, USA) medium containing 10% fetal bovine serum (G...

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Abstract

The present invention discloses application of circular RNA FOCAD (circ-FOCAD) as a new potential diagnostic biomarker and therapeutic target for gastric cancer and colorectal cancer. The Circ-FOCAD (hsa_circ_0008678|chr9:20923658-20933102+|FOCAD) is formed by reverse splicing of No.24-27 exons of a FOCAD gene with 555 bases. The invention prepares qRT-PCR primers of the circ-FOCAD and a small interfering RNA (siRNA) which interferes with the circ-FOCAD in vitro. Compared with normal controls, the circ-FOCAD is significantly up-regulated in both tumor and plasma samples from patients with gastric cancer and colorectal cancer. In vitro experiments show that the circ-FOCAD plays a role in cancer-promoting genes in gastric cancer and colorectal cancer cells. The results of the present study indicate that the circ-FOCAD is a novel potential biomarker and therapeutic target for gastric cancer and colorectal cancer.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to the application of circular RNA FOCAD as a diagnostic biomarker and therapeutic target for gastric cancer and colorectal cancer. Background technique [0002] Gastrointestinal tumors are the most common and dominant tumor types, including gastric cancer, colon cancer, and rectal cancer. According to the latest global cancer statistics report in 2018, among all malignant tumors, the death rate of gastric cancer ranks third in the world, and the incidence rate ranks fifth in the world; the mortality rate of colorectal cancer ranks second in the world, and the incidence rate ranks first in the world three. Current studies have shown that the occurrence of gastric cancer is related to regional environment and dietary factors, Helicobacter pylori infection, precancerous lesions, and genetic and genetic factors. The occurrence of colorectal cancer is related to high-fat and low-fiber d...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 李向南王振军
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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