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Method for detecting wall breaking rate of plant cells by utilizing field emission scanning electron microscopy

A scanning electron microscope and imaging detection technology, which is applied in the direction of material analysis using radiation, can solve problems such as experimental errors, difficulty in precise control, and calculation deviations, and achieve the effect of simple sample preparation and wide application range

Inactive Publication Date: 2019-04-16
湖南航天天麓新材料检测有限责任公司
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  • Application Information

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Problems solved by technology

However, it is difficult to verify the acquisition of completely broken samples and whether they are completely broken. In addition, small molecular substances, anions and cations, etc. may be released during the preparation process. Error and Calculation Bias

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  • Method for detecting wall breaking rate of plant cells by utilizing field emission scanning electron microscopy

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Embodiment Construction

[0019] Using field emission scanning electron microscope imaging to detect cell wall breakage rate is an intuitive, simple and quick detection method. The technical principle is that plant cells can be clearly imaged under a scanning electron microscope. After the cell wall is broken, the nutrients inside the plant cell are released, and the cell image with clear contrast and brightness disappears. The higher the breakage rate, the higher the breakage rate. The smaller the number of cells observed under the scanning electron microscope.

[0020] The process of the embodiment of the present invention is as follows:

[0021] A) Broken cell preparation steps: Get quantitative plant cells, put them in a wall breaker and crush them to prepare broken cells. The wall breaking machine is AUX-PB956 wall breaking cooking machine with the model of AUX-PB956. Its theoretical idling speed is 20000-40000 rpm / min. In this example, the idling speed of the crushing knife is 30,000 rpm / min. In thi...

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Abstract

The invention discloses a method for detecting a wall breaking rate of plant cells by utilizing field emission scanning electron microscopy. The cell wall breaking rate is detected by utilizing the field emission scanning electron microscope, the method can be used for accurately and rapidly realizing the field emission scanning electron microscope imaging technology to detect the wall breaking rate of the dried plant cells; and the method is simple, visual and rapid. The sample preparation process is simple, only a conductive adhesive needs to be adhered to a sample table, a cell suspension liquid is dropped on the conductive adhesive, and the whole can be placed into the field emission scanning electron microscope to be observed. The magnification factor of the scanning electron microscope can be adjusted randomly from 40 times to 1 million times, imaging is clear and cell characteristics can be observed; and the method is suitable for all dry plant raw materials, as long as the drying degree of the raw materials can be crushed by a common pulverizer to obtain more than 100 meshes, and the method is wide in application range.

Description

Technical field [0001] The invention relates to the field of food detection, in particular to a method for detecting plant cell wall breaking rate by using field emission scanning electron microscopy imaging. Background technique [0002] Plant cells are the basic unit of plant life activities and functions. They are composed of protoplasts and cell walls. Most nutrients are contained in the protoplasts. The presence of plant cell walls affects the human body's rapid absorption of nutrients in food to a certain extent. [0003] Therefore, the use of "wall breaking technology" to break the cell walls of plant cells can improve the body's ability to absorb nutrients and the rate of absorption. "Wall breaking technology" can be divided into mechanical method and non-mechanical method according to whether there is an external force; according to physical and non-physical methods, it can be divided into physical method and chemical method. According to the force, it can be divided int...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/04
CPCG01N23/04
Inventor 胡兴程菊红李志娟赵运哲张军生林美超王琪张劲
Owner 湖南航天天麓新材料检测有限责任公司
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