Method for constructing rat myocardial delayed ischemia reperfusion injury model

Abstract

The invention discloses a method for constructing a rat myocardial delayed ischemia reperfusion injury model, which comprises the following steps: (1) taking a rat for fasting overnight, anaesthetizing, passing through an oral trachea cannula through a 18G indwelling needle, connecting an outer sleeve with a breathing machine, and connecting four limbs with leads of an electrocardiograph; (2) longitudinally cutting skin from the left side of the sternum, lightly tearing the pericardium, inserting a needle at the root of the pulmonary artery cone, and discharging a needle thread at the position2-3mm below the left auricle; (3) placing a self-made rubber PE section on the surface of the heart at the ligation position, knotting on the rubber PE section, and performing continuous ligation ischemia for 1-6 hours in the myocardial infarction group; (4) opening the chest again to expose the ligation point, and observing the color of the anterior wall myocardium 15-20 seconds after the re-perfusion; suturing with 4-0 surgical suture after thoracotomy, continuous auxiliary ventilating and performing ibuprofen analgesia to obtain the rat myocardial delayed ischemia reperfusion injury model.According to the method, the natural rubber PE section is used as a medium for pressing the LAD in the model, so that the method has the advantages of rapid re-irrigation, visible ligation force andthe like; according to the method, a reperfusion injury model with high repeatable rate can be obtained after 4 hrs of ischemia.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing a rat myocardial delayed ischemia-reperfusion injury model. Background technique [0002] With the aging of the population, the incidence of myocardial infarction (MI) is also increasing year by year. Persistent ischemia caused by acute occlusion not only causes permanent damage to the myocardium, impaired ventricular systolic function, but also leads to the occurrence of chronic heart failure. With the continuous development of interventional technology, early reperfusion of damaged myocardium is the main treatment option. As a continuous pumping organ, the heart is particularly sensitive to ischemia and hypoxia. Even after effective reperfusion, cardiomyocytes still have obvious necrosis and apoptosis, which is called ischemia reperfusion injury (IRI). [0003] Under current conditions, the timely reperfusion of acute myocardial inf...

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Problems solved by technology

[0007] 1. There is no obvious anatomical structure reference for the position of the needle in and out, and it is difficult to keep the depth and width of the needle consistent, and it is often impossible to successfully ligate the LAD blood vessel at one time, resulting in the failure of modeling;

[0008] 2. When reperfusion needs to open the knot again, it will be pulled to the heart many times, and the risk of vascular injury, modeling failure and operation time will increase;

[0009] 3. The hard plastic tube (polyethylene tube) has a smooth and hard surface, so it is difficult to maintain consistent ligation tightness, which increases the risk of vascular injury;

[0010] 4. Previous methodologies did not explore and report on the feasible delayed reperfusion time

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