A kind of culture medium and its application of rapidly cultivating chlorella by stabilizing ph
A technology of chlorella and culture medium, applied in the field of microalgae growth, can solve problems such as high price, and achieve the effect of cost reduction and low cost
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Embodiment 1
[0029] Inoculate Chlorella luteorividis FACHB-1 (purchased from the Wildlife Germplasm Bank of Chinese Academy of Sciences in Wuhan—Freshwater Algae Species Bank) into GAAP and TAP medium respectively, and adjust the initial OD 680 =0.1, 30°C, light intensity 4000Lx, light-dark cycle 12:12h culture, record its growth every 12h (OD 680 ) and pH. Measurement results such as figure 1 As shown, when Chlorella luteorividis FACHB-1 grows in GAAP medium, its pH presents a trend of rising first and then falling, while in TAP medium, the pH keeps rising. Overall, the GAAP medium without pH buffer The pH of the culture solution can be well controlled between 7 and 8.5, which is beneficial to the rapid growth of Chlorella. On the other hand, the maximum biomass of Chlorella luteorividisFACHB-1 in GAAP and TAP medium was similar (OD 680 =2.4), but Chlorellaluteorividis FACHB-1 grew faster in GAAP medium. This result indicated that GAAP medium could better promote the growth of Chlorel...
Embodiment 2
[0031] Inoculate Chlorella protothecoides FACHB-3 (purchased from the Wild Organism Germplasm Bank of Chinese Academy of Sciences in Wuhan—Freshwater Algae Species Bank) into GAAP and TAP medium respectively, and adjust the initial OD 680 =0.1, cultured at 30°C, light intensity 4000Lx, light-dark cycle 12:12h, recorded its growth every 12h (OD 680 ) and pH. Measurement results such as figure 2 As shown, when Chlorella protothecoides FACHB-3 grows in GAAP medium, its pH presents a trend of rising first and then falling, while in TAP medium, the pH keeps rising. Overall, the GAAP medium without pH buffer The pH of the culture solution can be well controlled between 7 and 8.5, which is beneficial to the rapid growth of Chlorella. On the other hand, the maximum biomass of Chlorella protothecoidesFACHB-3 in GAAP and TAP medium was similar (OD 680 =2.5), but Chlorellaprotothecoides FACHB-3 grew faster in GAAP medium. This result indicated that GAAP medium could better promote the...
Embodiment 3
[0033] Inoculate Chlorella sacchrarophila FACHB-4 (purchased from the Wildlife Germplasm Bank of Chinese Academy of Sciences in Wuhan—Freshwater Algae Species Bank) into GAAP and TAP medium respectively, and adjust the initial OD 680 =0.1, cultured at 30°C, light intensity 4000Lx, light-dark cycle 12:12h, recorded its growth every 12h (OD 680 ) and pH. Measurement results such as image 3 As shown, when C. sacchrarophila FACHB-4 grows in GAAP medium, its pH rises first and then decreases, while in TAP medium, the pH keeps rising. Overall, the GAAP culture without pH buffer The base can well control the pH of the culture medium between 7 and 8.5, which is beneficial to the rapid growth of Chlorella. On the other hand, the maximum biomass of C. sacchrarophila FACHB-4 in GAAP and TAP medium was similar (OD 680 =2.4), but C. sacchrarophila FACHB-4 grows faster in GAAP medium, and can reach the maximum biomass after 48 hours of culture, while it needs 60 hours when cultured in T...
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