Preparation method of transparent soil material which can be used for soil animal activity observation

A soil and transparent technology, applied in the field of agriculture, can solve the problems of short survival time, low transparency, and complex ingredients, and achieve the effect of low toxicity of soil animals and simple and quick preparation

Active Publication Date: 2019-04-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition of the patent is complex, and soil animals such as earthworms survive in the soil for a short period of time. After testing, long-term observations cannot be carried out.
The reason may be that the carbon-nitrogen ratio or some component

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] Example 1

[0016] Weigh 10g of dried starch grafted acrylic particles, pulverize in a pulverizer, and finally make the particle size reach 1mm, mix well and wait for use; then add fish meal peptone and Ms medium into deionized water, where the content of fish meal peptone is 30g / L, the content of Ms medium is 4g / L, the mixed solution is sterilized at 121℃ for 30min to obtain Ms medium containing fish meal peptone; then the starch grafted acrylic acid and sterilized Ms containing fish meal peptone are cultured The mass-volume ratio of the base is 1:35. Weigh the starch-grafted acrylic particles of the corresponding mass into the corresponding Ms medium containing fish meal peptone. After the resin is completely swelled, a transparent soil is obtained. Putting earthworms and other soil animals into the transparent soil, the activity process of the earthworms in the soil can be seen with naked eyes, until the test is terminated two months later, the earthworms still live nor...

Example Embodiment

[0017] Example 2

[0018] Weigh 10g of dried starch grafted acrylic particles, pulverize in a pulverizer, and finally make the particle size reach 0.5mm, mix well and wait for use; then add fish meal peptone and Ms medium into deionized water, in which the content of fish meal peptone The content of Ms medium is 25g / L, and the content of Ms medium is 3g / L. The mixed solution is sterilized at 121°C for 20 minutes to obtain Ms medium containing fish meal peptone; then graft acrylic acid with starch and sterilized Ms containing fish meal peptone The mass-volume ratio of the medium is 1:30. Weigh the starch-grafted acrylic particles of the corresponding mass into the corresponding Ms medium containing fish meal peptone. After the resin is completely swelled, a transparent soil is obtained. Putting earthworms and other soil animals into the transparent soil, the activity process of the earthworms in the soil can be seen with naked eyes, until the test is terminated two months later, ...

Example Embodiment

[0019] Example 3

[0020] Weigh 10g of dried starch grafted acrylic particles, pulverize in a pulverizer, and finally make the particle size reach 0.8mm, mix well and wait for use; then add fish meal peptone and Ms medium into deionized water, in which the content of fish meal peptone The content of Ms medium is 35g / L, and the content of Ms medium is 5g / L. The mixed solution is sterilized at 121°C for 25min to obtain Ms medium containing fish meal peptone; then graft acrylic acid with starch and sterilized Ms containing fish meal peptone The mass-volume ratio of the medium is 1:45. Weigh the starch-grafted acrylic particles of the corresponding mass into the corresponding Ms medium containing fish meal peptone. After the resin is completely swelled, a transparent soil is obtained. Putting earthworms and other soil animals into the transparent soil, the activity process of the earthworms in the soil can be seen with naked eyes, until the test is terminated two months later, the e...

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Abstract

The invention discloses a preparation method of transparent soil material which can be used for soil animal activity observation; the transparent soil material is prepared by adding modified starch grafting acrylic resin into an Ms culture medium containing fish meal peptone; the modification means smashing starch grafting acrylic resin to the granularity of 0.5-1mm. According to the invention, the transparent soil material which can be used for soil animal activity observation can be prepared with a cheap and rapid method.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for preparing a transparent soil material. The prepared material can be used for observing the behavior of soil animals. Background technique [0002] Soil is the link between the water body and the atmosphere, and is one of the important components of the ecosystem. There are many animals, plants and microorganisms living in the soil. These organisms interact with the soil, and the soil quality can be quickly judged by the living conditions of the organisms. In the past, the activities of soil animals generally required destructive sampling, and the soil could only be seen after being separated. There are also certain technical means, such as CT scanning, but this method is complicated and costly, which is not conducive to popularization and use. After searching, it was found that there is a patent [Application No. 201611000726.0] that uses semi-permea...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K20/147A23K20/163A23K50/90
CPCA01K67/033A23K20/147A23K20/163A23K50/90
Inventor 李银生王亚利吴宜钊邱江平
Owner SHANGHAI JIAO TONG UNIV
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