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A kind of salvia miltiorrhiza endophytic fungus and its application in promoting the growth of salvia miltiorrhiza and/or synthesizing active ingredients

A technology of salvia miltiorrhiza endophytic fungus, salvia miltiorrhiza, applied in the field of microorganisms

Active Publication Date: 2020-10-27
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report in the literature that the separation of Salvia miltiorrhiza from Salvia miltiorrhiza can promote the growth of Salvia miltiorrhiza, and at the same time promote the synthesis of effective components of Danshen such as cryptotanshinone, tanshinone IIA and salvianolic acid B.

Method used

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  • A kind of salvia miltiorrhiza endophytic fungus and its application in promoting the growth of salvia miltiorrhiza and/or synthesizing active ingredients
  • A kind of salvia miltiorrhiza endophytic fungus and its application in promoting the growth of salvia miltiorrhiza and/or synthesizing active ingredients
  • A kind of salvia miltiorrhiza endophytic fungus and its application in promoting the growth of salvia miltiorrhiza and/or synthesizing active ingredients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Isolation and cultivation of endophytic fungi from Salvia miltiorrhiza

[0033] The endophytic fungus of Salvia miltiorrhiza of the present invention is separated and obtained according to the following steps:

[0034] 1) Immerse the seeds of Salvia miltiorrhiza in 75% ethanol for 1 minute, treat with 5% NaClO for 1 minute, wash with sterile water 3 times, each time for 1 minute, after sterilizing with 2.5% PBS-Tween20 solution, soak the seeds overnight;

[0035] 2) In a sterile environment, continue to rinse the test material obtained in step 1 with sterile water for 3 times, take 100 μl of the sterile water rinse solution from the last rinse, and inoculate it on a PDA plate, and incubate at 30°C for 24 hours. Perform sterility verification;

[0036] 3) If no colonies are produced, continue to the subsequent step 4; if colonies are produced, return to step 1 to continue the surface disinfection treatment;

[0037] 4) Place the previously surface-sterilized...

Embodiment 2

[0039] Embodiment 2: the acquisition of the aseptic tissue culture seedling of Salvia miltiorrhiza

[0040] 1) Select the full-grown seeds of Salvia miltiorrhiza, rinse with running water, treat with 75% alcohol, rinse with sterile water, treat with 5% NaClO solution, rinse with sterile water, absorb excess water with sterile filter paper, and sow in blank MS medium During the process, the aseptic vaccine of Salvia miltiorrhiza was obtained;

[0041] 2) After the aseptic seedlings grow and germinate, cut 1.5-2.0 cm stems with leaves and put them into MS medium for cultivation, and multiply to obtain aseptic tissue-cultured seedlings of Salvia miltiorrhiza.

Embodiment 3

[0042] Example 3: Binary co-cultivation of endophytic fungi of Salvia miltiorrhiza and sterile tissue culture seedlings

[0043] 1) Take the salvia miltiorrhiza endophytic fungus strain of the present invention, under aseptic conditions, pick a small amount of hyphae with an inoculation needle, insert it into a sterilized potato glucose liquid fermentation bottle, activate and cultivate it at 28°C for 6 days, and form bacterial suspension, so that the concentration of endophytic fungi in Salvia miltiorrhiza was 10 7 CFU / ml; Wherein the composition of potato glucose liquid culture medium is: potato 200g, glucose 20g, deionized water 1000mL;

[0044] 2) Seed the aseptic tissue culture seedlings of Salvia miltiorrhiza in the culture medium, inoculate 1ml of the bacterium suspension of the endophytic fungus of Salvia miltiorrhiza in the culture medium of the root, and inoculate 1ml of sterile potato dextrose liquid culture medium in the control group; Substrate: bury the roots of...

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Abstract

The invention relates to the technical field of microorganisms, and discloses radix salviae miltiorrhizae endophytic fungi and application thereof to promotion of growth of radix salviae miltiorrhizae / or synthesis of active ingredients. The invention provides the radix salviae miltiorrhizae endophytic fungi of which the preservation name is (phomaherbarum) D603, the preservation number is CGMCC No.16884, and the preservation unit is China center for microbial culture collection management committee general microbiology. Meanwhile, the invention also discloses application of the radix salviae miltiorrhizae endophytic fungi, and thus, the growth of the radix salviae miltiorrhizae can be effectively promoted, a main root of the radix salviae miltiorrhizae is thickened and lateral roots are developed, and the color of the surface of the root turns red; and moreover, the synthesis of tanshinone I, cryptotanshinone, dihydrotanshinone I, tanshinone IIA, tanshinol, rosmarinic acid and salvianolic acid B in the radix salviae miltiorrhizae can be effectively promoted.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an endophytic fungus of salvia miltiorrhiza and its application in promoting the growth of salvia miltiorrhiza and / or the synthesis and accumulation of active ingredients. Background technique [0002] Phomaherbarum is not only a pathogen of certain animals and plants, but also a biocontrol fungus that can be used for weed control. According to reports, Wu Qingyu and others have found that Phomophora phlegm is a pathogenic bacterium of some animals and plants, such as plant black shank and swim bladder fungal disease of salmon and trout (Wu Qingyu et al., Northeast Forestry University) Acta Sinica, 2005, 33(2): 43-45; Mohamed Faisal et al, 2007, Mycopathologia (2007) 163: 41-48), can also cause buckwheat ring spot disease, water lily black spot disease, etc.; and Gu Zumin et al. found that grass Phomophora is also a kind of biocontrol fungus that can be used for weed cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G7/06C12R1/45
Inventor 陈海敏梁宗锁吴红霞严彬余鸿宇张海花胡秀芳杨东风
Owner ZHEJIANG SCI-TECH UNIV
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