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Fermentation preparation method of two-step type cordyceps militaris enzyme combined with compound enzymatic hydrolysis

The technology of compound enzymolysis and Cordyceps militaris is applied in the research and development field of fermented beverages, which can solve the problems of long fermentation period and many raw materials, and achieve the effects of short fermentation period, unique flavor and improving fermentation efficiency.

Active Publication Date: 2019-05-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent also utilizes enzymatic hydrolysis and inoculation fermentation technology, due to the variety of raw materials used, the fermentation cycle is still long, and it needs to be fermented at 28-30°C for 22-24 days

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: A kind of rapid fermentation method of the Cordyceps militaris ferment combined with low ratio cellulase / pectinase compound enzymolysis

[0032] After removing impurities from the dry sample of Cordyceps militaris, use a high-speed pulverizer to pulverize and pass through a 100-mesh sieve to obtain Cordyceps militaris powder; mix the Cordyceps militaris powder with water at a ratio of 1:10, and stir evenly with a high-speed homogenizer to obtain Cordyceps militaris pulp; Add 300g of Cordyceps militaris slurry, add 150g of brown sugar, and add 550g of distilled water to quantify it to 1kg, stir fully to dissolve the brown sugar completely, and then pasteurize at 85°C for 10 minutes to obtain the fermentation substrate of Cordyceps militaris; wait for the fermentation substrate to cool Adjust the pH to 4.80±0.10 at 40-45°C, add 0.4% cellulase and 0.6% pectinase, and inoculate 1g of compound lactic acid bacteria powder, and carry out compound enzymatic hydroly...

Embodiment 2

[0035] Embodiment 2: A kind of rapid fermentation method of the Cordyceps militaris ferment combined with equal proportion cellulase / pectinase compound enzymolysis

[0036] After removing impurities from the dry sample of Cordyceps militaris, use a high-speed pulverizer to pulverize and pass through a 100-mesh sieve to obtain Cordyceps militaris powder; mix the Cordyceps militaris powder with water at a ratio of 1:10, and stir evenly with a high-speed homogenizer to obtain Cordyceps militaris pulp; Add 300g of Cordyceps militaris slurry, add 150g of brown sugar, and add 550g of distilled water to quantify it to 1kg, stir fully to dissolve the brown sugar completely, and then pasteurize at 85°C for 10 minutes to obtain the fermentation substrate of Cordyceps militaris; wait for the fermentation substrate to cool Adjust the pH to 4.80±0.10 at 40-45°C, add 0.5% cellulase and 0.5% pectinase, and inoculate 1g of compound lactic acid bacteria powder, and carry out compound enzymolysi...

Embodiment 3

[0039] Embodiment 3: A kind of rapid fermentation method of the Cordyceps militaris enzyme combined with high ratio cellulase / pectinase compound enzymolysis

[0040] After removing impurities from the dry sample of Cordyceps militaris, use a high-speed pulverizer to pulverize and pass through a 100-mesh sieve to obtain Cordyceps militaris powder; mix the Cordyceps militaris powder with water at a ratio of 1:10, and stir evenly with a high-speed homogenizer to obtain Cordyceps militaris pulp; Add 300g of Cordyceps militaris slurry, add 150g of brown sugar, and add 550g of distilled water to quantify it to 1kg, stir fully to dissolve the brown sugar completely, and then pasteurize at 85°C for 10 minutes to obtain the fermentation substrate of Cordyceps militaris; wait for the fermentation substrate to cool Adjust the pH to 4.80±0.10 at 40-45°C, add 0.6% cellulase and 0.4% pectinase, and inoculate 1g of compound lactic acid bacteria powder, and carry out compound enzymolysis and l...

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PUM

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Abstract

The present invention discloses a fermentation preparation method of a two-step type cordyceps militaris enzyme combined with a compound enzymatic hydrolysis, and belongs to the technical field of research and development of fermented beverages. Cordyceps militaris powder and cordyceps militaris slurry preparation, cordyceps militaris fermentation substrate preparation, compound enzymatic hydrolysis cooperation with lactic acid bacterium fermentation and other processes are conducted, and then centrifugation, filtration, blending, sterilization and filling are conducted to prepare the product.The compound enzymatic hydrolysis cooperation with the lactic acid bacterium fermentation is utilized, then second fermentation of yeasts is conducted, the whole fermentation process can be completedquickly in 2-3 days, and the obtained cordyceps militaris enzyme is sweet, sour, tasty, unique in flavor and good in suspension stability, can also well retain an important active ingredient of cordycepin: a dissolution amount of the cordycepin in the cordyceps militaris powder can reach 78.21% and a preservation rate of the dissolved-out cordycepin is up to 97.78%, has a certain antioxidant activity and immunity-improving function, and is in line with modern people's pursuits of healthy and delicious high-quality beverages.

Description

technical field [0001] The invention relates to a two-step fermentation preparation method of cordyceps militaris enzyme combined with compound enzymolysis, in particular to a method that uses brown sugar as a carbon source, utilizes compound enzymolysis to promote the fermentation of lactic acid bacteria, and then inoculates yeast for secondary fermentation. The fermentation method belongs to the technical field of research and development of fermented beverages. Background technique [0002] Cordyceps militaris is a parasitic fungus that has been listed as a new resource food in 2009. It contains a variety of nutrients, such as protein and amino acids, Cordyceps polysaccharide, cordycepic acid (D-mannitol), cordycepin and superoxide dismutation Enzymes (SOD), etc. Ancient book records and modern research show that the medicinal value of Cordyceps militaris is similar to that of Cordyceps sinensis, and comparable to that of ginseng and antler. Its unique cordycepin can be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L2/84
CPCA23L2/84A23L2/382A23V2400/125A23V2400/113A23V2400/531A23V2400/169
Inventor 张慜劳艳艳阳武雄易思富侯丽华郭小玲
Owner JIANGNAN UNIV