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A method for microalgae to start compensatory growth at ice temperature

A technology for compensating growth and microalgae, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problems of high production costs, low breeding efficiency, short breeding period, etc., and achieve industrial production. The effect of low efficiency and low processing cost

Active Publication Date: 2021-07-16
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, in the world, the cultivation of microalgae basically adopts the backward cultivation method of traditional greenhouse runway cultivation, which makes the cultivation efficiency of the whole region low and the production cost high
[0005] In order to solve the problems of low aquaculture production efficiency, low biomass during aquaculture period, short aquaculture period and difficult breeding, combined with the local reality, research on aquaculture technology in autumn and winter was carried out, and a method for microalgae to start supercompensatory growth at ice temperature was found, in order to improve Total output value, reduce production costs, and obtain maximum economic benefits

Method used

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  • A method for microalgae to start compensatory growth at ice temperature

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Effect test

Embodiment 1

[0057] This embodiment is a method of spiral algae ice temperature activation compensation growth, including the following steps:

[0058] 1) Incorporate the jacchite inoculated into a fresh Zhi culture medium at a cultivation concentration of 10 ml / L, cultured for 2 days at 32 ° C, 6000lux, pH = 9, which adapted to a new growth environment. For several days;

[0059] 2) Under Dark Condition, the culture temperature of the spiral algae in which the long-term long-term spiral algae is reduced at a rate of 1 ° C to -5 ° C at a rate of 1 ° C per 36 minutes; and the culture temperature is maintained for 12 h.

[0060] The spirulina cultured in the above step continued to cultivate 4 days at 32 ° C, 6000 lux. The day relative growth rate is detected, and the relative growth rate (OD) 0.210 is measured.

Embodiment 2

[0062] This embodiment is a method of laminating a saline algae ice temperature, including the following steps:

[0063] 1) Incorporate saline alga in a fresh DM medium in a fresh DM medium in a fresh DM medium, cultured under 32 ° C, 6000lux, pH = 7.5, which adapted to a new growth environment and enters logarithm growth. period.

[0064] 2) Under Dark Condition, the culture temperature in which a long period of saline alga in which a long period of life is lowered at a rate of 1 ° C per 36 minutes, and the culture temperature is maintained at a rate of 9 h.

[0065] The saline alga cultured in the above steps was continued for 4 days at 32 ° C, 6000 lux. It is detected that the day relative growth rate is detected, and the day relative growth rate (OD) 0.168 is measured.

Embodiment 3

[0067] This embodiment is a method of taking a chromophoreglutaneous ice temperature activation compensation growth, including the following steps:

[0068] 1) Inoculated into fresh BG-11 medium in a fresh BG-11 medium in a fresh BG-11 medium in the concentration of 10 ml / L, it was cultured under the culture conditions of 32 ° C, 6000 lux, pH = 7.1, so that it adapted to a new growth environment. For a long time.

[0069] 2) Under Dark Condition, the culture temperature of the chlorinated algae in which the long-term long-term chlorinated algae is reduced at a rate of 1 ° C to -5 ° C at a rate of 1 ° C per 36 minutes; the culture temperature is maintained.

[0070] The collaborate cultured in the above steps was continued for 4 days at 32 ° C, 6000 lux. It is detected that the day relative growth rate is detected, and the day relative growth rate (OD) 0.089 is measured.

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Abstract

The invention relates to a method for starting and compensating growth of microalgae at ice temperature. The method specifically comprises: under dark conditions, the culture temperature of microalgae in the logarithmic growth phase is uniformly reduced to 0-10°C, and the temperature is maintained. After 1-15h, you can. The invention induces changes in the internal absorption and metabolism mechanism of the microalgae through external ice temperature stimulation, activates the ice temperature compensation growth mechanism, and promotes the vitality of the body. The invention is simple and effective, has low processing cost, can be directly used in the industrialized production of microalgae, greatly improves the industrialized yield of microalgae, and the growth rate of microalgae treated by the method of the present invention is more than 2 times higher than that of untreated microalgae. This has great popularization and application value for improving the efficiency of microalgae cultivation, prolonging the cultivation period and large-scale cultivation.

Description

Technical field [0001] The present invention relates to a method of increasing the growth rate of microggal, and more particularly to a method of activating a microal algae ice temperature start compensation growth. Background technique [0002] Spirulina, saline and chiocana are economical microalgae that can have a large-scale industrialization, rich in protein, fat, vitamin, biological polysaccharides, unsaturated fatty acids, chlorophyll, carotenoids and humans. Material, etc., has a good nutritional health function. At present, the microalgae food treatment tablet is one of the leading products, and there is still a food that has added algae powder nutrient extract to the ordinary food to prepare a fine algae nutrition and flavor. On the other hand, in a few days of consuming in the fossil fuel, biofuels are becoming more and more important, and the microalga biofuels are third-generation biofuels, the first generation of creatures produced by crops such as starch and corn p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/36C12N1/12C12N1/20C12R1/89
Inventor 李博生常蓉吕冰心
Owner BEIJING FORESTRY UNIVERSITY