Method for detecting ethrel

Abstract

The invention discloses a method for detecting ethrel. The method includes the following steps: ethrel in a sample is extracted; a detection solution of ethrel derivatives is prepared, the sample is extracted through an analytical reagent, then sulfuric acid is used as a protective agent, heptafluorobutanol and heptafluorobutyric anhydride are used as derivatization reagents to make the ethrel inthe sample derive into the derivatives under the temperature condition of 60-120 DEG C, and then a chromatography reagent is added to obtain the detection solution; a derivative ethrel working solution is prepared; GC-MS / MS chromatographic analysis is conducted; an external standard method is used for quantification, the mass concentration of a target object is used as an abscissa, the corresponding peak area is used as an ordinate, and a standard curve is drawn. The ethrel is stable in the process of derivation, the speed of an esterification reaction is high, the speed of derivation is high,sensitivity is high, qualitative and quantitative detection is accurate, operation is easy, use of hypertoxic reagent diazomethane can be avoided, and the environment is protected.

Description

technical field [0001] The invention relates to the field of detection methods, in particular to a method for detecting ethephon. Background technique [0002] Ethephon is a plant growth regulator, which has the physiological effects of plant hormones to promote emulsion secretion, accelerate maturation, shedding, aging and flowering. Under certain conditions, ethephon can not only release ethylene itself, but also induce plants to produce ethylene, which can be used as an agricultural plant growth stimulant. Ethephon (Ethephon) is a high-quality and high-efficiency plant growth regulator. One molecule of Ethephon can release one molecule of ethylene, which has the effects of promoting fruit ripening, stimulating wound flow, and regulating gender transformation. Its molecular structure is shown in figure 1 . [0003] Since previous studies have shown that ethephon has low toxicity and is very effective in accelerating crop ripening and increasing crop yield, ethephon is wi...

AI Technical Summary

Problems solved by technology

The advantages of the diazomethane method are mild reaction conditions (just under room temperature), rapid reaction (reaction time 15min), high derivatization rate, etc., but the disadvantage is that diazomethane is too toxic and carcinogenic. There is no commercial product for methane, and it needs to be prepared by the laboratory itself, which is cumbersome and has a short shelf life of only one month. In addition, because the derivative product of the diazomethane method is dimethylethephon (molecular weight 172.5), the molecular weight is relatively small, so if With gas analysis, the sample matrix will have great interference, and it is difficult to use for trace analysis. Therefore, the national standard stipulates that gas chromatography with FPD should be used for detection, which can only be qualitatively determined by the retention time of the chromatographic peak, which has certain limitations in qualitative aspects. defect

[0007] The difficulty of derivation of ethephon is that once the substance is formulated into a solution, it needs to be relatively stable in acidic conditions. When the pH>3, ethephon becomes unstable and easy to decompose; the market needs a solution to the instability of ethephon in the derivation process , the esterification reaction is carried out slowly, and the method for detecting ethephon with low sensitivity; the present invention solves these problems

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