Method for increasing germination rate of stevia rebaudiana seeds
A technology of stevia and germination rate, applied in the field of seed treatment in the field of agricultural technology, can solve the problems of restricting stevia yield, low emergence rate, low germination rate, etc., achieve no residual cost, increase germination rate, promote germination and seedling growth The effect of growth
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Embodiment 1
[0017] Embodiment 1 determines optimum concentration and optimal pretreatment time
[0018] Gently rub the stevia seeds with your hands to remove the pappus, remove the seeds and impurities by winnowing, select the stevia seeds with plump and healthy seeds and the same size, weigh 54 parts of 20g seeds, wash them with distilled water 4 times, Rinse well; then disinfect with 10% sodium hypochlorite solution for 10 minutes, rinse with distilled water 3 times, and filter dry.
[0019] Prepare different concentrations of salicylic acid to pretreat stevia seeds; the concentration of salicylic acid is 0, 0.03, 0.05, 0.10, 0.50, 1.00mmol / L; take 100mL of different concentrations of salicylic acid for each 3 (3 replicates ) x 3 (3 pretreatment time) portions, add the same amount of sterilized stevia seeds respectively, and soak the seeds in a constant temperature water bath at 25°C for 12, 24, and 36 hours respectively; after the soaking, rinse the seeds with distilled water for 3 tim...
Embodiment 2
[0026] The seeds of Huinong No. 3 stevia rebaudiana propagated by Gansu Agricultural Engineering Technology Research Institute were selected, 2000 grains were randomly divided into 1000 grains. Among them, 1000 seeds were mixed with an aqueous solution with a salicylic acid concentration of 0.05mmol / L; the other 1000 seeds were used as a control, soaked in clean water at a ratio of 1:10 for 24 hours at 25°C, then washed with clean water and air-dried seed. The seed germination test was carried out by Petri dish filter paper method. Each group of seeds that have been processed is sowed evenly on the filter paper in a petri dish (diameter 90mm) that has been wetted with distilled water and the water content is 60%. 100 seeds per dish are repeated 3 times and sprayed every day. 1 time of water, keep the filter paper moist, cover the Petri dish and put it in the incubator for cultivation. The culture temperature is 25°C, the humidity is 85%, the light is 12h / d, and the light int...
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