63 results about "Stevia pilosa" patented technology

The invention discloses a method for preparing a stevia rebaudiana biological growth promoting agent and belongs to the field of agriculture and comprehensive utilization. In the preparation method, the stevia rebaudiana biological growth promoting agent is prepared by taking stevia rebaudiana waste residues and stevia rebaudiana stems obtained after stevia is extracted as raw materials and adding inorganic salt with trace elements and microbial composite strains for carrying out fermentation. The stevia rebaudiana biological growth promoting agent prepared by the method can be used as a fertilizer and a feed additive. When the stevia rebaudiana biological growth promoting agent is used as the fertilizer, the yield of crops and sweetness can be promoted and soil can be improved. When the stevia rebaudiana biological growth promoting agent is used as the feed additive, the quality and the yield of animal meat can be promoted and the antiviral ability of animals is improved. The stevia rebaudiana biological growth promoting agent has the characteristics of high efficiency, low cost, no toxicity, no residues, environmental protection and the like. The method for preparing the stevia rebaudiana biological growth promoting agent has high application value.

The invention relates to a glossy ganoderma sugar-free beverage and a production method thereof, belonging to the non-alcoholic beverage. The glossy ganoderma sugar-free beverage comprises the following components by weight percent: 18% of dry glossy ganoderma, 1.5% of honeysuckle, 1.5% of prunella vulgaris, 1.5% of hawthorn, 1.2% of Chinese wolfberry, 1.2% of red jujube, 0.1% of stevia rebaudiana and the balance purified water, wherein dry glossy ganoderma is dry glossy ganoderma powder with a grain size of no more than 0.30mm; glossy ganoderma, honeysuckle and prunella vulgaris are all dry products, the water contents are all less than 13%; and red jujube, Chinese wolfberry and stevia rebaudiana are all dry products, and the water contents are all less than 18%. The production method ofthe glossy ganoderma sugar-free beverage comprises the following steps: 1) pretreating raw materials; 2) heating and extracting; 3) performing fine filtration, canning; 4) sterilizing, cooling; and 5) inspecting. No any preservative is added in the beverage and the raw materials used in the beverage are natural plants and have good functional accomodation function to human body, thus the beverageof the invention belongs to a healthy and green beverage; and the beverage is suitable for the middle-aged and elder people to drink and especially for people with diabetes.

The invention discloses the application of stevia rebaudiana in the aspect of preparing reconstituted tobacco and the reconstituted tobacco. The stevia rebaudiana is extracted through alcohol to obtain stevia rebaudiana extractum and/or stevia rebaudiana fibers, one or both the stevia rebaudiana extractum and the stevia rebaudiana fibers can be used for preparing the reconstituted tobacco, the reconstituted tobacco can be endowed with special fragrance, the irritation can be reduced, and the smoking taste can be improved. The effective ingredients of the stevia rebaudiana are fully utilized, the smoking quality of the cigarette is improved, meanwhile, the releasing amount of CO, tar and other ingredients in the cigarette is reduced, and a powerful technological foundation is provided for adding new tobacco products and reducing the tobacco harm. The raw materials are easy to obtain, the reconstituted tobacco is safe and healthy, and popularization and application are facilitated.

Stevia compositions are prepared from steviol glycosides of Stevia Rebaudiana Bertoni. The compositions are able to provide a superior taste profile and can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

The invention discloses a feed capable of guaranteeing high disease resistance of princess chicken. The feed is prepared from the following raw materials in parts by weight: 50-70 parts of corn flour, 10-14 parts of bran, 3-5 parts of pumpkin powder, 2-3 parts of shrimp shell powder, 4-6 parts of tofu dregs, 1-2 parts of stevia rebaudiana, 4-6 parts of chaff, 1-2 parts of shaddock peels, 3-4 parts of dried vinasse, 6-10 parts of rape seed cakes, 10-20 parts of soybean straws, 1-2 parts of rhodiola rosea, 1-2 parts of leaves of paniculate microcos, 1-2 parts of fig leaves, 0.5-1 part of clausena lansium, 1-2 parts of radix rehmanniae recen, 1-2 parts of four leaf clover, 0.6-1 part of desmodium triquetrum and 1-2 parts of additives. The feed disclosed by the invention is prepared form the easily available raw materials, is low in cost, safe and free from toxic and side effects, and can improve the disease resistance and immunity of the princess chicken to a great extent, reduce the morbidity and mortality and lower the culture cost. The added additives can further improve the taste, accelerate food taking of the princess chicken and promote the growing speed.

The invention discloses lipid-lowering and blood-pressure-lowering tea. The tea is prepared from the following pure natural plant Chinese medicinal herbs by weight: 20 to 40kg of fiveleaf gynostemma herb, 20 to 40kg of kudzuvine root, 15 to 25kg of flower of kudzuvine, 15 to 25kg of wild chrysanthemum, 15 to 25kg of honeysuckle flower, 15 to 25kg of Stevia rebaudiana, 15 to 25kg of Indian buead, and 15 to 25kg of Mongolian snakegourd root. A preparation method comprises the following steps of: cutting stems and leaves of the fiveleaf gynostemma herb and the Stevia rebaudiana into small sections, cutting the kudzuvine root, the Indian buead and the Mongolian snakegourd root into pieces, adding whole flowers of the flower of kudzuvine, the wild chrysanthemum and the honeysuckle flower, and mixing and loading into bags according to the proportion, wherein the weight of each bag is 50 to 100g. The tea is directly stewed by using boiling water before drinking, and juice is drunk after tea leaves are removed. According to the feedback of long-term users, the tea has the effects of promoting urination and lowering lipid and blood pressure.

The invention discloses a stevia rebaudiana solid health care tea. The stevia rebaudiana solid health care tea is characterized in that stevia rebaudiana water extracting solution powder, lemon grass water extracting solution powder, jujube kernel powder and water are mixed to be uniform, tabletting and vacuum drying are performed to obtain the stevia rebaudiana solid health care tea, and the ratio of the stevia rebaudiana water extracting solution powder to the lemon grass water extracting solution powder to the jujube kernel powder to the water is 15-18:5-8:4-6:2-3. The stevia rebaudiana solid health care tea is simple in formula, and fragrant and sweet in taste, can achieve sterilization and virus resistance, reduce lipid and pressure, nourish yin and soothe the nerves, further has the beautifying effect, and has the high health care effect.

The invention relates to corn stigma and germinated brown rice tea and a preparation method thereof, which belong to the field of beverage. The invention aims to solve the technical problems that the functional components of current corn stigma tea are difficult to dissolve, and single corn stigma component is not ideal in blood glucose reducing effect. The product is prepared by corn stigma, germinated brown rice, tea and rebaudiana. The method comprises the steps of: 1, edulcorating, cleaning and dehydrating corn stigma sequentially, and then drying ultrasonically and smashing; 2, roasting germinated brown rice, and naturally cooling and smashing the germinated brown rice; and 3, smashing tea and rebaudiana; 4, weighing corn stigma, germinated brown rice, tea and rebaudiana processed in the above steps respectively; and 5, weighing the materials and uniformly mixing the materials and obtaining the corn stigma and germinated brown rice tea. The dissolving-out amount of the functional components of corn stigma are effectively improved, and the functional components of corn stigma and germinated brown rice are interacted, so that the blood glucose and blood fat reducing effect of the tea provided by the invention is remarkably improved compared with that of current corn stigma tea.

The invention discloses a method for improving RA content of stevia rebaudiana through in-vitro culture. The method comprises the following steps: inoculating stevia rebaudiana leaves onto a callus induced medium, transferring the produced callus into a differential medium, forming buds, transferring into a rooting medium, growing a root system, acclimatizating and transplanting into a greenhouse,and performing routine cultivation management. The invention belongs to the technical fields of cytobiology and biology. The invention provides a method for rapidly obtaining stevia rebaudiana plantswith high RA content, the content of the RA in the stevia rebaudiana leaves is increased, and the taste is excellent.

The invention relates to longevity healthcare tea which is a healthcare beverage. The longevity healthcare tea is produced from 1g to 25g of dendrobe, 1g to 30g of tuckahoe, 1g to 35g of sevenleaf gynostemma herb, 2g to 40g of alpinia japonica, 1g to 15g of acanthopanax, 2g to 40g of astragalus membranaceus and 1g to 15g of stevia rebaudiana. The longevity healthcare tea can be drunk after being soaked in boiled water of 80 DEG C above for more than 5min, or the ingredients are ground into powder which is then stored in a small tea bag which can be immersed, the powder is sub-packaged by10g, 20g, 50g and the like, and the bagged tea can be drunk after being soaked in boiled water at the temperature of more than 80 DEG C for more than 5min. The longevity healthcare tea has the functions oftonifying the spleen, nourishing the stomach, supplementing the Qi for strengthening superficies, nourishing Yin, supplementing the liver and kidney, enriching the essence and blood, soothing the nerves and the like according to the traditional Chinese medicine theory; the longevity healthcare tea has the functions of resisting the fatigue, resisting the oxidation, promoting the sleep, improving the immunity of a human body and the like according to the modern pharmacology study and also has an auxiliary function for reducing the blood pressure, blood sugar and blood fat, resisting the cancerand resisting the aging.

The invention relates to the technical field of agriculture plantation, and concretely relates to a herbicide used for stevia rebaudiana plantation and a preparation method thereof. The herbicide comprises the following components: Setaria viridis, aconitum sinomontanum nakai, euphorbia, artemisia argyi, bidens pilosa, euphorbia helioscopia, golden larch bark, angled bittersweet, tripterygium wilfordii, radix sophorae flavescentis and rheum officinale; the preparation method comprises the following steps: respectively weighing the components, crushing the components, uniformly mixing the components; adding the components in a soxhlet extractor, adding ethanol, performing ultrasonic-assisted extraction, taking the mixing components to obtain an extract; placing the mixing components in a flask, adding water, performing ultrasonic-assisted heat backflow, filtering the material while hot, repetitively operating for 2 times; merging the obtained extract, a filtrate and a washing lotion, and concentrating the materials to obtain the target product herbicide. The herbicide used for stevia rebaudiana plantation can effectively remove the weeds, and can inhibit germination of weed seeds, and the preparation method is simple, operation is convenient, and the method is suitable for popularization and application.

The invention discloses a fruity corn stigma beverage which comprises the following raw materials in parts by weight: 60 to 80 parts of corn stigma juice, 15 to 30 parts of fresh orange juice, 1 to 10 parts of fruit granules, 0.5 to 5 parts of a traditional Chinese medicine concentrated solution, 1 to 8 parts of stevia rebaudiana, 0.1 to 2 parts of citric acid and appropriate amount of purified water. According to the invention, corn stigma juice is used as a main raw material, then fresh orange juice and fruit granules that are rich in nutrition and good in taste are added, and then the traditional Chinese medicine concentrated solution with heat clearing and lipid-decreasing effects and the green and natural stevia rebaudiana are matched, so that the prepared fruity corn stigma beverage not only has the fragrance of the corn stigma, but also is thick in fruit flavors, the taste is fragrant, sweet and unique, the fruity corn stigma beverage is an excellent health-caring beverage, the sources of the raw materials are wide, the production cost is low, and the market prospect is wide.

The invention belongs to the field of genetic engineering, specifically to a production method for chlorogenic acid and dicaffeoylquinic acid by establishing a hairy root culture system through genetic transformation of stevia rebaudiana with agrobacterium rhizogenes. The method comprises the following steps: preparation of an explant of stevia rebaudiana, activation and culture of agrobacterium rhizogenes, suspension culture and production of chlorogenic acid and dicaffeoylquinic acid. PCR detection results show that hairy roots of stevia rebaudiana are generated through transformation; HPLC detection results show that the hairy roots of stevia rebaudiana can produce chlorogenic acid and dicaffeoylquinic acid. Since the hairy roots used in the invention have the characteristic of rapid growth on a hormone-free medium, the method provided by the invention has the advantages of simple operation, low cost, no restriction by natural conditions like climate and land, etc. A stable and sustainable novel drug source and food function factors are provided for production of chlorogenic acid and dicaffeoylquinic acid through culture of the hairy roots, and a reliable source and a reliable substance base are provided for large scale production of chlorogenic acid and dicaffeoylquinic acid by using a bioreactor in the future.

The invention belongs to the technical field of processing of substitutional tea and particularly relates to a processing method of pelleted substitutional polygonatum kingianum tea. According to thesubstitutional tea, the characteristics that polygonatum kingianum is hot in nature and tartary buckwheat is cold in nature are utilized, warm nature is achieved by combining polygonatum kingianum andtartary buckwheat according to a certain proportion, stevia rebaudiana is added to improve the functionality and the taste, and the pelleted substitutional polygonatum kingianum tea which is warm innature, unlikely to be dispersed during brewing in boiling water, clean and bright in soup color and capable of moistening the mouth is prepared without adding any other sticking substance. The processing method includes the following steps of 1, cleaning, slicing, drying, smashing and sieving polygonatum kingianum tubers for use; 2, hulling but not peeling tartary buckwheat and then drying, smashing and sieving tartary buckwheat for use; 3, drying, smashing and sieving stevia rebaudiana for use; 4, adding water into prepared tartary buckwheat powder for stirring and uniform mixing and then adding prepared polygonatum kingianum powder and stevia rebaudiana powder for stirring and uniform mixing again; 5, putting the uniformly mixed material into an extrusion forming pelleting machine for extrusion forming; 6, putting the formed particles into a stainless steel baking tray, then putting the baking tray into a baking oven for baking, and then taking out the baking tray for cooling to obtain the pelleted substitutional polygonatum kingianum tea.

A stevia rebaudiana glucoside extracting method is provided. The method includes a step of drying stevia rebaudiana leaves in the sun, and crushing the leaves to obtain stevia rebaudiana crude powder; a step of adding the crude powder into a Soxhlet extractor, adding diethyl ether into a flask connected to the Soxhlet extractor, heating and refluxing the mixture for 3-4 h, and taking the crude powder out to obtain an extract liquid; a step of adding the crude powder into a flask, adding methanol, extracting the crude powder through ultrasonic assisted hot refluxing for 60-80 min, performing filtration when the solution is still hot, and washing the filter residues; a step of adding the filter residues into a flask, adding water into the filter residues, extracting the filter residues through ultrasonic assisted hot refluxing for 30-40 min, performing filtration when the solution is still hot, and repeating the operation twice; and a step of combining the obtained filtrates and washing liquids, and performing rotary evaporation to obtain the target product that is the stevia rebaudiana glucoside. According to the method, operation is simple, common agents are used, the cost is low, the stevia rebaudiana glucoside is high in purity, extracting time is short, stevioside extraction is thorough and the method is suitable for industrial application.

The invention provides a stevia rebaudiana Bertoni stalk and alfalfa mixed and wrapped silage feed and a preparation method thereof. The method comprises the following steps of (1) harvesting raw materials: recovering utilized abandoned stevia rebaudiana Bertoni stalks, and harvesting alfalfa from the squaring stage to the early flowering stage; (2) performing kneading, cutting up and mixing: uniformly mixing the harvested stevia rebaudiana Bertoni stalks with the harvested alfalfa, and performing kneading and cutting up through machinery; (3) performing binding: performing high-density compacting and binding on the cut-up silage raw materials; (4) performing wrapping: performing wrapping and sealing on the bound silage raw materials; and (5) performing storage fermentation. According to the stevia rebaudiana Bertoni stalk and alfalfa mixed and wrapped silage feed disclosed by the invention, the stevia rebaudiana Bertoni stalks and the alfalfa are selected as silage raw materials for mixed silage, so that the problem that the alfalfa is low in soluble carbohydrate content, high in buffering energy and difficult in direct ensile can be solved; besides, stevia rebaudiana Bertoni waste residues, stevia rebaudiana Bertoni stalks and the like are also utilized, so that resource waste is reduced, and environmental pollution is reduced; and besides, the source of the silage feed is extended, and the nutrient quality of the silage raw materials is guaranteed.

The invention relates to an extraction method of rebaudioside A, a Stevia rebaudiana Bertoni extract and an extraction method thereof, belonging to the food chemical industry field. The invention aims to provide the method for extracting the high-purity and high-yield rebaudioside A from Stevia rebaudiana Bertoni. The method comprises the following steps: a. carrying out continuous countercurrent extraction on leaves and stem of the Stevia rebaudiana Bertoni and a water-methanol mixed solvent at 50-80 DEG C based on the proportion of 1:3-1:9m/v, wherein, flow velocity of the water-methanol mixed solvent on a Stevia rebaudiana Bertoni raw material per kilogram is 1-4L/h; b. concentrating the obtained extracting solution to obtain a crude rebaudioside A product, dispersing the crude product with water, and then ultra-filtering to obtain filtrate; c. adsorbing and eluting the obtained ultrafiltrate with polar resin, and then eluting with the water-methanol mixed solvent; and d. concentrating the obtained eluent, freezing, stirring and crystallizing the obtained concentrated solution at the temperature of -20 DEG C for 24 hours, freezing, stirring and recrystallizing at the temperature of -20 DEG C for 24 hours, collecting and drying crystal at 80 DEG C to obtain the rebaudioside A with purity up to not less than 99% and yield up to more than 85%. The obtained rebaudioside A has no adverse remaining taste on mouthfeel, large treatment capacity and low cost, thus being applicable to industrial production.

The invention provides a method for preparing fermented stevia rebaudiana tea. The method comprises the steps of selecting stevia rebaudiana plants growing well and harvesting the stevia rebaudiana plants; selecting fresh blades good in color and free of insect damage; placing the stevia rebaudiana blades in a temperature control chamber to perform withering until the leaves are soft; collecting the stevia rebaudiana blades and placing the stevia rebaudiana blades in a rolling machine to perform rolling, and performing rolling to a strip-shaped rolling state; placing the well rolled stevia rebaudiana blades in the temperature control chamber to perform fermentation, until the leaf red stain rate reaches 90% or above; drying the rolled stevia rebaudiana blades, and obtaining the fermented stevia rebaudiana tea. The method for preparing the fermented stevia rebaudiana tea is simple in process and convenient to operate; according to the fermented stevia rebaudiana tea obtained through the preparation method, not only can nutrient ingredients of stevia rebaudiana be released by brewing, but also the taste is good; and the fermented stevia rebaudiana tea has important application values. In addition, the fermented stevia rebaudiana tea has no additive ingredient, is all natural and safe to drink, and has good practical values.

The invention discloses a method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria and application, and belongs to the technical field of phytopathogen separation and identification. The method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria comprises the steps that development, evolution analysis, identification and purification of a morphological observation and molecular biological identification combination system are performed, and the species of the stevia rebaudiana leaf spot disease pathogenic bacteria subjected to pathogenicity identification is subjected to molecular biology identification and system development and evolution analysis as ITS, GAPDH and CHS-1 gene segments, and after homologous comparison, system development evolution trees for the ITS, GAPDH and CHS-1 gene segments are constructed respectively. The method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria is rapid and convenient to implement, and identification errors caused by the inter-species close height are avoided; a foundation is laid for identifying, preventing and breeding the stevia rebaudiana leaf spot disease.

The invention discloses a method for increasing the germination rate of stevia rebaudiana seeds. The method has the following specific steps: selecting the stevia rebaudiana seeds with plump and healthy seeds in the same size for later use; disinfecting the seeds for 5-15 minutes by virtue of a 10% sodium hypochlorite solution, repeatedly flushing the seeds with distilled water, and filtering to dry; preparing salicylic acid in different concentrations, and carrying out pretreatment on the stevia rebaudiana seeds; respectively adding equivalent disinfected stevia rebaudiana seeds into at leastthree parts of equivalent salicylic acid in different concentrations so as to soak the seeds; and after the soaking of the seeds is finished, flushing the seeds for 3-5 times, and carrying out seed germination test by virtue of a culture dish filter paper method. The method disclosed by the invention has the advantages of convenience, high efficiency, no toxicity, no residue, low cost and the like, is high in economic benefit and convenient for agricultural production and has wide application prospects in the modern efficient agricultural production.