63 results about "Stevia pilosa" patented technology

The invention discloses a method for preparing a stevia rebaudiana biological growth promoting agent and belongs to the field of agriculture and comprehensive utilization. In the preparation method, the stevia rebaudiana biological growth promoting agent is prepared by taking stevia rebaudiana waste residues and stevia rebaudiana stems obtained after stevia is extracted as raw materials and adding inorganic salt with trace elements and microbial composite strains for carrying out fermentation. The stevia rebaudiana biological growth promoting agent prepared by the method can be used as a fertilizer and a feed additive. When the stevia rebaudiana biological growth promoting agent is used as the fertilizer, the yield of crops and sweetness can be promoted and soil can be improved. When the stevia rebaudiana biological growth promoting agent is used as the feed additive, the quality and the yield of animal meat can be promoted and the antiviral ability of animals is improved. The stevia rebaudiana biological growth promoting agent has the characteristics of high efficiency, low cost, no toxicity, no residues, environmental protection and the like. The method for preparing the stevia rebaudiana biological growth promoting agent has high application value.

The invention relates to a glossy ganoderma sugar-free beverage and a production method thereof, belonging to the non-alcoholic beverage. The glossy ganoderma sugar-free beverage comprises the following components by weight percent: 18% of dry glossy ganoderma, 1.5% of honeysuckle, 1.5% of prunella vulgaris, 1.5% of hawthorn, 1.2% of Chinese wolfberry, 1.2% of red jujube, 0.1% of stevia rebaudiana and the balance purified water, wherein dry glossy ganoderma is dry glossy ganoderma powder with a grain size of no more than 0.30mm; glossy ganoderma, honeysuckle and prunella vulgaris are all dry products, the water contents are all less than 13%; and red jujube, Chinese wolfberry and stevia rebaudiana are all dry products, and the water contents are all less than 18%. The production method ofthe glossy ganoderma sugar-free beverage comprises the following steps: 1) pretreating raw materials; 2) heating and extracting; 3) performing fine filtration, canning; 4) sterilizing, cooling; and 5) inspecting. No any preservative is added in the beverage and the raw materials used in the beverage are natural plants and have good functional accomodation function to human body, thus the beverageof the invention belongs to a healthy and green beverage; and the beverage is suitable for the middle-aged and elder people to drink and especially for people with diabetes.

The invention discloses a potting domestication method for traditional Chinese medicine stevia rebaudiana. The method comprises the steps of seed guiding domestication, tissue culturing, potting managing, industrialized production, nursery discharging of finished products and the like. According to the potting domestication method for the traditional Chinese medicine stevia rebaudiana, the stevia rebaudiana can be better converted to potted flowers from field-planted flowers, can be appreciated by people, is high in survival rate, simple and easy to operate and low in cost, the economic values of the stevia rebaudiana is improved and the method is suitable for large-scale promotion.

The invention discloses the application of stevia rebaudiana in the aspect of preparing reconstituted tobacco and the reconstituted tobacco. The stevia rebaudiana is extracted through alcohol to obtain stevia rebaudiana extractum and / or stevia rebaudiana fibers, one or both the stevia rebaudiana extractum and the stevia rebaudiana fibers can be used for preparing the reconstituted tobacco, the reconstituted tobacco can be endowed with special fragrance, the irritation can be reduced, and the smoking taste can be improved. The effective ingredients of the stevia rebaudiana are fully utilized, the smoking quality of the cigarette is improved, meanwhile, the releasing amount of CO, tar and other ingredients in the cigarette is reduced, and a powerful technological foundation is provided for adding new tobacco products and reducing the tobacco harm. The raw materials are easy to obtain, the reconstituted tobacco is safe and healthy, and popularization and application are facilitated.

Stevia compositions are prepared from steviol glycosides of Stevia Rebaudiana Bertoni. The compositions are able to provide a superior taste profile and can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

The invention discloses a feed capable of guaranteeing high disease resistance of princess chicken. The feed is prepared from the following raw materials in parts by weight: 50-70 parts of corn flour, 10-14 parts of bran, 3-5 parts of pumpkin powder, 2-3 parts of shrimp shell powder, 4-6 parts of tofu dregs, 1-2 parts of stevia rebaudiana, 4-6 parts of chaff, 1-2 parts of shaddock peels, 3-4 parts of dried vinasse, 6-10 parts of rape seed cakes, 10-20 parts of soybean straws, 1-2 parts of rhodiola rosea, 1-2 parts of leaves of paniculate microcos, 1-2 parts of fig leaves, 0.5-1 part of clausena lansium, 1-2 parts of radix rehmanniae recen, 1-2 parts of four leaf clover, 0.6-1 part of desmodium triquetrum and 1-2 parts of additives. The feed disclosed by the invention is prepared form the easily available raw materials, is low in cost, safe and free from toxic and side effects, and can improve the disease resistance and immunity of the princess chicken to a great extent, reduce the morbidity and mortality and lower the culture cost. The added additives can further improve the taste, accelerate food taking of the princess chicken and promote the growing speed.

The invention discloses tea with functions of reducing blood sugar and losing weight and a production method thereof. The tea is prepared from the materials such as large bitter gourd, radix polygonati officinalis, bitter physalis pubescens, dandelion, green tea, houttuynia cordata, humifuse euphorbia herb, radix puerariae, winter mulberry leaves, common fenugreek seeds, miracle fruit tree leaves and stevia rebaudiana. The main process comprises the following steps of selecting the materials, drying, crushing, batching, removing impurities, sterilizing, quantifying, and packaging to obtain a finished product. The tea and the production method disclosed by the invention have the beneficial effects that on the basis of the secret prescription handed down from ancestors, by taking and clinical verification of multiple cases as well as continuous summarization and perfection, the formula is more complete, the curative effect is exact, and the effect for the main diseases such as dry throat and thirst, internal-heat consumptive thirst and dysphoric-fever consumptive thirst is obvious; the effects for nourishing yin and moistening dryness, clearing heat and removing toxicity, promoting production of body fluid and quenching thirst, warming kidney and activating yang and eliminating cold and stopping pain are achieved to different degrees; and the tea has the advantages of health care, reduction of blood sugar, weight losing, pure nature and no toxic or side effect and the like.

The invention discloses lipid-lowering and blood-pressure-lowering tea. The tea is prepared from the following pure natural plant Chinese medicinal herbs by weight: 20 to 40kg of fiveleaf gynostemma herb, 20 to 40kg of kudzuvine root, 15 to 25kg of flower of kudzuvine, 15 to 25kg of wild chrysanthemum, 15 to 25kg of honeysuckle flower, 15 to 25kg of Stevia rebaudiana, 15 to 25kg of Indian buead, and 15 to 25kg of Mongolian snakegourd root. A preparation method comprises the following steps of: cutting stems and leaves of the fiveleaf gynostemma herb and the Stevia rebaudiana into small sections, cutting the kudzuvine root, the Indian buead and the Mongolian snakegourd root into pieces, adding whole flowers of the flower of kudzuvine, the wild chrysanthemum and the honeysuckle flower, and mixing and loading into bags according to the proportion, wherein the weight of each bag is 50 to 100g. The tea is directly stewed by using boiling water before drinking, and juice is drunk after tea leaves are removed. According to the feedback of long-term users, the tea has the effects of promoting urination and lowering lipid and blood pressure.

The invention relates to a method for extracting stevia rebaudiana sugar by using a mixed flocculant. The method comprises the following steps of: soaking stevia rebaudiana leaves in clear water, extracting for four times, and extracting the stevia rebaudiana sugar by adding flocculants which are required to be diluted first and then are added, namely heating extracting solution of the stevia rebaudiana sugar, adding solution of the flocculant A and stirring, adding saturated solution of the flocculant B and stirring, adding lime water to regulate the pH value of the mixed flocculant to be between 9 and 9.5, stirring, standing for 10 minutes, injecting the obtained solution into a plate and frame filter for filtration, loading filtrate on an adsorption column for absorption, analyzing, desalinizing and decolorizing analytical liquid, concentrating the desalinized and decolorized analytical liquid until certain concentration is obtained, and spray-drying. The organic and inorganic mixed flocculant is used to replace a pure inorganic salt flocculant, the formula is simple, the cost is reduced, flocculation effect is strengthened and the purity of the stevia rebaudiana sugar is improved; compared with an inorganic salt flocculation method, the method has the advantage that: the content of total glucosides is improved from 80-85 percent to 90 percent; and the method is easy to operate and is favorable for promotion.

The invention discloses a stevia rebaudiana solid health care tea. The stevia rebaudiana solid health care tea is characterized in that stevia rebaudiana water extracting solution powder, lemon grass water extracting solution powder, jujube kernel powder and water are mixed to be uniform, tabletting and vacuum drying are performed to obtain the stevia rebaudiana solid health care tea, and the ratio of the stevia rebaudiana water extracting solution powder to the lemon grass water extracting solution powder to the jujube kernel powder to the water is 15-18:5-8:4-6:2-3. The stevia rebaudiana solid health care tea is simple in formula, and fragrant and sweet in taste, can achieve sterilization and virus resistance, reduce lipid and pressure, nourish yin and soothe the nerves, further has the beautifying effect, and has the high health care effect.

The invention relates to corn stigma and germinated brown rice tea and a preparation method thereof, which belong to the field of beverage. The invention aims to solve the technical problems that the functional components of current corn stigma tea are difficult to dissolve, and single corn stigma component is not ideal in blood glucose reducing effect. The product is prepared by corn stigma, germinated brown rice, tea and rebaudiana. The method comprises the steps of: 1, edulcorating, cleaning and dehydrating corn stigma sequentially, and then drying ultrasonically and smashing; 2, roasting germinated brown rice, and naturally cooling and smashing the germinated brown rice; and 3, smashing tea and rebaudiana; 4, weighing corn stigma, germinated brown rice, tea and rebaudiana processed in the above steps respectively; and 5, weighing the materials and uniformly mixing the materials and obtaining the corn stigma and germinated brown rice tea. The dissolving-out amount of the functional components of corn stigma are effectively improved, and the functional components of corn stigma and germinated brown rice are interacted, so that the blood glucose and blood fat reducing effect of the tea provided by the invention is remarkably improved compared with that of current corn stigma tea.

The invention discloses a method for improving RA content of stevia rebaudiana through in-vitro culture. The method comprises the following steps: inoculating stevia rebaudiana leaves onto a callus induced medium, transferring the produced callus into a differential medium, forming buds, transferring into a rooting medium, growing a root system, acclimatizating and transplanting into a greenhouse,and performing routine cultivation management. The invention belongs to the technical fields of cytobiology and biology. The invention provides a method for rapidly obtaining stevia rebaudiana plantswith high RA content, the content of the RA in the stevia rebaudiana leaves is increased, and the taste is excellent.

The invention relates to longevity healthcare tea which is a healthcare beverage. The longevity healthcare tea is produced from 1g to 25g of dendrobe, 1g to 30g of tuckahoe, 1g to 35g of sevenleaf gynostemma herb, 2g to 40g of alpinia japonica, 1g to 15g of acanthopanax, 2g to 40g of astragalus membranaceus and 1g to 15g of stevia rebaudiana. The longevity healthcare tea can be drunk after being soaked in boiled water of 80 DEG C above for more than 5min, or the ingredients are ground into powder which is then stored in a small tea bag which can be immersed, the powder is sub-packaged by10g, 20g, 50g and the like, and the bagged tea can be drunk after being soaked in boiled water at the temperature of more than 80 DEG C for more than 5min. The longevity healthcare tea has the functions oftonifying the spleen, nourishing the stomach, supplementing the Qi for strengthening superficies, nourishing Yin, supplementing the liver and kidney, enriching the essence and blood, soothing the nerves and the like according to the traditional Chinese medicine theory; the longevity healthcare tea has the functions of resisting the fatigue, resisting the oxidation, promoting the sleep, improving the immunity of a human body and the like according to the modern pharmacology study and also has an auxiliary function for reducing the blood pressure, blood sugar and blood fat, resisting the cancerand resisting the aging.

The invention relates to the technical field of agriculture plantation, and concretely relates to a herbicide used for stevia rebaudiana plantation and a preparation method thereof. The herbicide comprises the following components: Setaria viridis, aconitum sinomontanum nakai, euphorbia, artemisia argyi, bidens pilosa, euphorbia helioscopia, golden larch bark, angled bittersweet, tripterygium wilfordii, radix sophorae flavescentis and rheum officinale; the preparation method comprises the following steps: respectively weighing the components, crushing the components, uniformly mixing the components; adding the components in a soxhlet extractor, adding ethanol, performing ultrasonic-assisted extraction, taking the mixing components to obtain an extract; placing the mixing components in a flask, adding water, performing ultrasonic-assisted heat backflow, filtering the material while hot, repetitively operating for 2 times; merging the obtained extract, a filtrate and a washing lotion, and concentrating the materials to obtain the target product herbicide. The herbicide used for stevia rebaudiana plantation can effectively remove the weeds, and can inhibit germination of weed seeds, and the preparation method is simple, operation is convenient, and the method is suitable for popularization and application.

The invention discloses a fruity corn stigma beverage which comprises the following raw materials in parts by weight: 60 to 80 parts of corn stigma juice, 15 to 30 parts of fresh orange juice, 1 to 10 parts of fruit granules, 0.5 to 5 parts of a traditional Chinese medicine concentrated solution, 1 to 8 parts of stevia rebaudiana, 0.1 to 2 parts of citric acid and appropriate amount of purified water. According to the invention, corn stigma juice is used as a main raw material, then fresh orange juice and fruit granules that are rich in nutrition and good in taste are added, and then the traditional Chinese medicine concentrated solution with heat clearing and lipid-decreasing effects and the green and natural stevia rebaudiana are matched, so that the prepared fruity corn stigma beverage not only has the fragrance of the corn stigma, but also is thick in fruit flavors, the taste is fragrant, sweet and unique, the fruity corn stigma beverage is an excellent health-caring beverage, the sources of the raw materials are wide, the production cost is low, and the market prospect is wide.

The invention belongs to the field of genetic engineering, specifically to a production method for chlorogenic acid and dicaffeoylquinic acid by establishing a hairy root culture system through genetic transformation of stevia rebaudiana with agrobacterium rhizogenes. The method comprises the following steps: preparation of an explant of stevia rebaudiana, activation and culture of agrobacterium rhizogenes, suspension culture and production of chlorogenic acid and dicaffeoylquinic acid. PCR detection results show that hairy roots of stevia rebaudiana are generated through transformation; HPLC detection results show that the hairy roots of stevia rebaudiana can produce chlorogenic acid and dicaffeoylquinic acid. Since the hairy roots used in the invention have the characteristic of rapid growth on a hormone-free medium, the method provided by the invention has the advantages of simple operation, low cost, no restriction by natural conditions like climate and land, etc. A stable and sustainable novel drug source and food function factors are provided for production of chlorogenic acid and dicaffeoylquinic acid through culture of the hairy roots, and a reliable source and a reliable substance base are provided for large scale production of chlorogenic acid and dicaffeoylquinic acid by using a bioreactor in the future.

The invention belongs to the technical field of processing of substitutional tea and particularly relates to a processing method of pelleted substitutional polygonatum kingianum tea. According to thesubstitutional tea, the characteristics that polygonatum kingianum is hot in nature and tartary buckwheat is cold in nature are utilized, warm nature is achieved by combining polygonatum kingianum andtartary buckwheat according to a certain proportion, stevia rebaudiana is added to improve the functionality and the taste, and the pelleted substitutional polygonatum kingianum tea which is warm innature, unlikely to be dispersed during brewing in boiling water, clean and bright in soup color and capable of moistening the mouth is prepared without adding any other sticking substance. The processing method includes the following steps of 1, cleaning, slicing, drying, smashing and sieving polygonatum kingianum tubers for use; 2, hulling but not peeling tartary buckwheat and then drying, smashing and sieving tartary buckwheat for use; 3, drying, smashing and sieving stevia rebaudiana for use; 4, adding water into prepared tartary buckwheat powder for stirring and uniform mixing and then adding prepared polygonatum kingianum powder and stevia rebaudiana powder for stirring and uniform mixing again; 5, putting the uniformly mixed material into an extrusion forming pelleting machine for extrusion forming; 6, putting the formed particles into a stainless steel baking tray, then putting the baking tray into a baking oven for baking, and then taking out the baking tray for cooling to obtain the pelleted substitutional polygonatum kingianum tea.

A stevia rebaudiana glucoside extracting method is provided. The method includes a step of drying stevia rebaudiana leaves in the sun, and crushing the leaves to obtain stevia rebaudiana crude powder; a step of adding the crude powder into a Soxhlet extractor, adding diethyl ether into a flask connected to the Soxhlet extractor, heating and refluxing the mixture for 3-4 h, and taking the crude powder out to obtain an extract liquid; a step of adding the crude powder into a flask, adding methanol, extracting the crude powder through ultrasonic assisted hot refluxing for 60-80 min, performing filtration when the solution is still hot, and washing the filter residues; a step of adding the filter residues into a flask, adding water into the filter residues, extracting the filter residues through ultrasonic assisted hot refluxing for 30-40 min, performing filtration when the solution is still hot, and repeating the operation twice; and a step of combining the obtained filtrates and washing liquids, and performing rotary evaporation to obtain the target product that is the stevia rebaudiana glucoside. According to the method, operation is simple, common agents are used, the cost is low, the stevia rebaudiana glucoside is high in purity, extracting time is short, stevioside extraction is thorough and the method is suitable for industrial application.

The invention discloses a method for remotely transporting stevia rebaudiana seedlings. The seedlings with 6-8 leaves and the plant height being 10-15 cm in a call-out place can be requested to be subjected to dew-free seedlings lifting and straw banding within a stevia rebaundiana seedlings call-in place transplanting optimum period, ventilating hard material boxes can be adopted for packaging, the stevia rebaudiana seedlings packaging boxes can be placed in a vehicle refrigerating storage in order and occupy 80% space of the vehicle refrigerating storage, the temperature of the vehicle refrigerating storage can be controlled to be 7-9 DEG C, the stevia rebaudiana seedlings can be better placed in a call-in place and can be in complete fix planting within 48 hours after arriving, and the survival rate of the seedlings after the transplanting can be up to more than 85%. The method disclosed by the invention is more suitable for remotely transporting the stevia rebaudiana seedlings.

The invention provides a stevia rebaudiana Bertoni stalk and alfalfa mixed and wrapped silage feed and a preparation method thereof. The method comprises the following steps of (1) harvesting raw materials: recovering utilized abandoned stevia rebaudiana Bertoni stalks, and harvesting alfalfa from the squaring stage to the early flowering stage; (2) performing kneading, cutting up and mixing: uniformly mixing the harvested stevia rebaudiana Bertoni stalks with the harvested alfalfa, and performing kneading and cutting up through machinery; (3) performing binding: performing high-density compacting and binding on the cut-up silage raw materials; (4) performing wrapping: performing wrapping and sealing on the bound silage raw materials; and (5) performing storage fermentation. According to the stevia rebaudiana Bertoni stalk and alfalfa mixed and wrapped silage feed disclosed by the invention, the stevia rebaudiana Bertoni stalks and the alfalfa are selected as silage raw materials for mixed silage, so that the problem that the alfalfa is low in soluble carbohydrate content, high in buffering energy and difficult in direct ensile can be solved; besides, stevia rebaudiana Bertoni waste residues, stevia rebaudiana Bertoni stalks and the like are also utilized, so that resource waste is reduced, and environmental pollution is reduced; and besides, the source of the silage feed is extended, and the nutrient quality of the silage raw materials is guaranteed.

The invention discloses a chestnut flower health drink and a preparation method thereof. The chestnut flower health drink is formed by mixing a chestnut flower flavone extracting solution, stevia rebaudiana, malic acid, citric acid, crystal sugar, oligosaccharide syrup, sodium alga acid and water. The drink takes the chestnut flower flavone extracting solution as a raw material and has soft taste, pleasant flavor and the effects of clearing away heat and toxic materials, resisting oxidation, suppressing bacteria and resisting tumors; high-sweetness low-sugar stevia rebaudiana is combined with the chestnut flower flavone extracting solution, so that the health effect and nutritive value of the drink are enhanced, and the drink has a certain curative effect on diabetes, hypertension, obesity and heart diseases; and due to addition of citric acid, malic acid, crystal sugar and oligosaccharide syrup, the drink is sour, sweet and tasty and is suitable for people of all ages; and due to addition of sodium alga acid, the drink can be prevented from being layered. According to the method, the chestnut flower is deeply processed, and the flavone extracting solution is prepared into the drink, so that the additional value of the chestnut flower is effectively utilized, a healthy drink with high nutrition and good mouthfeel is obtained, and the drink is simple in production process and low in cost and has good economic benefits.

The invention relates to an extraction method of rebaudioside A, a Stevia rebaudiana Bertoni extract and an extraction method thereof, belonging to the food chemical industry field. The invention aims to provide the method for extracting the high-purity and high-yield rebaudioside A from Stevia rebaudiana Bertoni. The method comprises the following steps: a. carrying out continuous countercurrent extraction on leaves and stem of the Stevia rebaudiana Bertoni and a water-methanol mixed solvent at 50-80 DEG C based on the proportion of 1:3-1:9m / v, wherein, flow velocity of the water-methanol mixed solvent on a Stevia rebaudiana Bertoni raw material per kilogram is 1-4L / h; b. concentrating the obtained extracting solution to obtain a crude rebaudioside A product, dispersing the crude product with water, and then ultra-filtering to obtain filtrate; c. adsorbing and eluting the obtained ultrafiltrate with polar resin, and then eluting with the water-methanol mixed solvent; and d. concentrating the obtained eluent, freezing, stirring and crystallizing the obtained concentrated solution at the temperature of -20 DEG C for 24 hours, freezing, stirring and recrystallizing at the temperature of -20 DEG C for 24 hours, collecting and drying crystal at 80 DEG C to obtain the rebaudioside A with purity up to not less than 99% and yield up to more than 85%. The obtained rebaudioside A has no adverse remaining taste on mouthfeel, large treatment capacity and low cost, thus being applicable to industrial production.

The invention provides a method for preparing fermented stevia rebaudiana tea. The method comprises the steps of selecting stevia rebaudiana plants growing well and harvesting the stevia rebaudiana plants; selecting fresh blades good in color and free of insect damage; placing the stevia rebaudiana blades in a temperature control chamber to perform withering until the leaves are soft; collecting the stevia rebaudiana blades and placing the stevia rebaudiana blades in a rolling machine to perform rolling, and performing rolling to a strip-shaped rolling state; placing the well rolled stevia rebaudiana blades in the temperature control chamber to perform fermentation, until the leaf red stain rate reaches 90% or above; drying the rolled stevia rebaudiana blades, and obtaining the fermented stevia rebaudiana tea. The method for preparing the fermented stevia rebaudiana tea is simple in process and convenient to operate; according to the fermented stevia rebaudiana tea obtained through the preparation method, not only can nutrient ingredients of stevia rebaudiana be released by brewing, but also the taste is good; and the fermented stevia rebaudiana tea has important application values. In addition, the fermented stevia rebaudiana tea has no additive ingredient, is all natural and safe to drink, and has good practical values.

The present invention discloses a stevia rebaudiana and oat germ enzyme and black fungus beverage and a preparation method thereof. Each 1000 g of the beverage comprises an enzyme raw liquid by fermenting 0.72-0.84 g of the stevia rebaudiana and using the oat germ as a carbon source, black fungus broken grains manufactured by 20-35 g of the black fungus, and the balance being RO water. The stevia rebaudiana and the black fungus are used as raw materials, the raw materials are subjected to a microorganism fermentation, the nutritional essence of the stevia rebaudiana is preserved, and at the same time, the nutritional benefits of the stevia rebaudiana are fully played. The beverage is rich in a variety of active enzymes, vitamins, minerals and microbial metabolism products needed by human body, good in mouthfeel, and low in sugar content, has functions of lowering blood sugar and lipids, and preventing thrombosis, tumors and oxidation and other important physiological functions, and is suitable for a long-term drinking and brings a healthy and innovative product to consumers. The preparation method is mild in reaction, low in production cost, simple and easily controllable in operation processes, and easy to implement large-scale industrial production.

The invention discloses a method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria and application, and belongs to the technical field of phytopathogen separation and identification. The method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria comprises the steps that development, evolution analysis, identification and purification of a morphological observation and molecular biological identification combination system are performed, and the species of the stevia rebaudiana leaf spot disease pathogenic bacteria subjected to pathogenicity identification is subjected to molecular biology identification and system development and evolution analysis as ITS, GAPDH and CHS-1 gene segments, and after homologous comparison, system development evolution trees for the ITS, GAPDH and CHS-1 gene segments are constructed respectively. The method for rapidly identifying stevia rebaudiana leaf spot disease pathogenic bacteria is rapid and convenient to implement, and identification errors caused by the inter-species close height are avoided; a foundation is laid for identifying, preventing and breeding the stevia rebaudiana leaf spot disease.

The invention relates to a preparation method of a vernonia amygdalina Del. leaf tea beverage, and belongs to the technical field of beverages with health-care functions. The method mainly comprises the following steps: carrying out mixed crushing on camellia oleifera Abel. leaves, vernonia amygdalina Del. leaf and stevia leaf, adding soda water, and conducting ultrasonic-assisted decocting; chopping raw ginger, adding water with the weight being 10 times the weight of the ginger, conducting ultrasonic-assisted decocting, and subjecting the decocted ginger juice to rotary evaporation until thevolume decreases by half of original volume so as to obtain a ginger juice concentrate; pulverizing green tea, conducting sieving with a 60-mesh sieve, adding water with the weight being 15 times theweight of the sieved green tea for decocting, conducting suction filtration, and collecting supernatant as a green tea solution; combining an extractive solution, the ginger juice concentrate and thegreen tea solution, stirring and uniformly mixing so as to obtain a mixed solution; and subjecting the mixed solution to sterilization and filling so as to obtain the vernonia amygdalina Del. leaf tea beverage. The tea beverage prepared by the method does not add any food additive such as preservative, color matching agent, flavoring agent in addition to the own components of the product, and hasthe advantages of being natural in taste, clear in color, good in acceptability, free from side effects and fragrant in flavor.

The invention provides a low-sugar healthcare cake, and belongs to the technical field of food processing. The healthcare cake is prepared from, by weight, 100-200 parts of low-gluten flour, 100-200 parts of egg white, 70-120 parts of yolk, 50-80 parts of buckwheat flour, 10-20 parts of fruit juice, 10-30 parts of cake oil, 20-40 parts of liquid maltitol, 15-30 parts of stevia rebaudiana, 5-15 parts of folium eriobotryae, 5-10 parts of folium mori, 5-10 parts of poria cocos, 5-10 parts of Chinese waxgourd seed, 5-10 parts of licorice root, 2-6 parts of typhonium giganteum and an appropriate amount of water. According to the healthcare cake, maltitol and stevia rebaudiana are used for replacing white sugar, and stevia rebaudiana is free of sugar or calories and has the advantages of being high in sweetness and low in calorie; by adding the fruit juice, the cake is unique in flavor, delicious and tasty; as stevia rebaudiana, folium eriobotryae, folium mori, poria cocos, Chinese waxgourdseed, licorice root and typhonium giganteum are added, the cake has the healthcare effects of clearing away heat and toxic materials, promoting the circulation of qi, harmonizing stomach, tonifying the spleen and the kidneys and relieving exterior syndrome through pungent and warm features.

The invention discloses a protection method for stevia rebaudiana stubble to safely overwinter and relates to a technology that winter protection and warm keeping are performed on stevia rebaudiana during overwintering so as to enable the stevia rebaudiana to safely overwinter. The protection method comprises the steps: when the overground part of the stevia rebaudiana growing in a field just die of freeze, covering the stevia rebaudiana in the field by utilizing crop straws by certain thickness; and when weather gets warm again and the lowest air temperature is stably kept to be above 0 DEG C, removing the crop straws. The overwintering protection technology of the stevia rebaudiana is not provided with any facility, and is low in cost and reliable in effect.

The invention discloses a nutrient breakfast food and a preparation method thereof. The nutrient breakfast food is prepared from red dates, red beans, mung beans, Chinese yam, lotus seeds, ginger, soya beans, corn, walnut kernels, black sesames, Caulis Sinomenii and Stevia Rebaudiana Bertoni. Compared with nutrient breakfast foods prepared through the prior art, the nutrient breakfast food of the invention allows the compatibility of medicinal and edible traditional Chinese medicinal materials with different colors of red, green, white, black and the like to be carried out to achieve a purpose of nutrition supplement of different internal organs, and a certain amount of Caulis Sinomenii is added to make the breakfast food have wind dispelling, vein relaxing, dehumidifying and pain relieving effects and be suitable for middle aged and elderly populations to eat, and a proper amount of Stevia Rebaudiana Bertoni is also added to adjust the mouthfeel, so the nutrient breakfast food can prevent diabetes and hypertension after eating for a long term.

The invention discloses a method for increasing the germination rate of stevia rebaudiana seeds. The method has the following specific steps: selecting the stevia rebaudiana seeds with plump and healthy seeds in the same size for later use; disinfecting the seeds for 5-15 minutes by virtue of a 10% sodium hypochlorite solution, repeatedly flushing the seeds with distilled water, and filtering to dry; preparing salicylic acid in different concentrations, and carrying out pretreatment on the stevia rebaudiana seeds; respectively adding equivalent disinfected stevia rebaudiana seeds into at leastthree parts of equivalent salicylic acid in different concentrations so as to soak the seeds; and after the soaking of the seeds is finished, flushing the seeds for 3-5 times, and carrying out seed germination test by virtue of a culture dish filter paper method. The method disclosed by the invention has the advantages of convenience, high efficiency, no toxicity, no residue, low cost and the like, is high in economic benefit and convenient for agricultural production and has wide application prospects in the modern efficient agricultural production.