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A stevia rebaudiana glucoside extracting method

A stevioside and extraction method technology, applied in the field of stevioside extraction, can solve the problems of rough separation and purification process, uncontrollable stevioside quality, incomplete glycoside extraction, etc., achieve thorough glycoside extraction, short extraction time, low cost effect

Inactive Publication Date: 2017-05-10
MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, stevia leaves are mainly used in stevia rebaudiana, but the research on steviol glycosides has disadvantages such as uncontrollable quality, rough separation and purification process, and incomplete glycoside extraction. Therefore, research is to find a method that can effectively extract stevioside glycosides of great significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This embodiment relates to a method for extracting steviol glycosides, comprising the following steps:

[0024] (1) Stevia leaves are dried in the sun and pulverized with a pulverizer to a particle size of 100 mesh to obtain stevia coarse powder;

[0025] (2) Wrap 5 g of stevia powder with filter paper and add it to a Soxhlet extractor, add 100 mL of ether into a flask connected to the Soxhlet extractant, heat and reflux at 75°C for 4 hours, take out the stevia powder, and obtain the extract ;

[0026] (3) Put the above-mentioned stevia coarse powder into a flask, add 100mL of methanol, at 68°C, under the condition of ultrasonic radiation of 100W, extract by ultrasonic-assisted thermal reflux for 70min, filter while hot, and wash;

[0027] (4) Put the filter residue in a flask, add 100mL of water, at 95°C, under the condition of ultrasonic radiation of 100W, then extract by ultrasonic-assisted thermal reflux for 30min, filter while it is hot, and repeat the operation t...

Embodiment 2

[0030] This embodiment relates to a method for extracting steviol glycosides, comprising the following steps:

[0031] (1) Stevia leaves are dried in the sun and pulverized to a particle size of 50 mesh with a pulverizer to obtain coarse stevia powder;

[0032] (2) Wrap 8g of stevia coarse powder with filter paper and add it to a Soxhlet extractor, add 120mL of ether into a flask connected to the Soxhlet extractant, heat and reflux at 72°C for 3h, take out the stevia coarse powder, and extract liquid;

[0033] (3) Put the above stevia coarse powder into a flask, add 80mL of methanol, at 70°C, under the condition of ultrasonic radiation of 100W, extract by ultrasonic-assisted thermal reflux for 60min, filter while hot, and wash;

[0034] (4) Put the filter residue in a flask, add 80mL of water, at 95°C, under the condition of ultrasonic radiation of 100W, then extract by ultrasonic-assisted heat reflux for 30min, filter while it is hot, and repeat the operation twice;

[0035...

Embodiment 3

[0037] This embodiment relates to a method for extracting steviol glycosides, comprising the following steps:

[0038] (1) Stevia leaves are dried in the sun and pulverized with a pulverizer to a particle size of 100 mesh to obtain stevia coarse powder;

[0039] (2) Wrap 5g of stevia coarse powder with filter paper and add it to a Soxhlet extractor, add 120mL of ether into a flask connected to the Soxhlet extractant, heat and reflux at 75°C for 4h, take out the stevia coarse powder, and extract liquid;

[0040] (3) Put the above-mentioned stevia coarse powder into a flask, add 100mL of methanol, at 65°C, under the condition of ultrasonic radiation of 90W, extract by ultrasonic-assisted thermal reflux for 80min, filter while hot, and wash;

[0041] (4) Put the filter residue in a flask, add 100mL of water, at 100°C, under the condition of ultrasonic radiation of 90W, then extract by ultrasonic-assisted heat reflux for 40min, filter while it is hot, and repeat the operation twi...

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Abstract

A stevia rebaudiana glucoside extracting method is provided. The method includes a step of drying stevia rebaudiana leaves in the sun, and crushing the leaves to obtain stevia rebaudiana crude powder; a step of adding the crude powder into a Soxhlet extractor, adding diethyl ether into a flask connected to the Soxhlet extractor, heating and refluxing the mixture for 3-4 h, and taking the crude powder out to obtain an extract liquid; a step of adding the crude powder into a flask, adding methanol, extracting the crude powder through ultrasonic assisted hot refluxing for 60-80 min, performing filtration when the solution is still hot, and washing the filter residues; a step of adding the filter residues into a flask, adding water into the filter residues, extracting the filter residues through ultrasonic assisted hot refluxing for 30-40 min, performing filtration when the solution is still hot, and repeating the operation twice; and a step of combining the obtained filtrates and washing liquids, and performing rotary evaporation to obtain the target product that is the stevia rebaudiana glucoside. According to the method, operation is simple, common agents are used, the cost is low, the stevia rebaudiana glucoside is high in purity, extracting time is short, stevioside extraction is thorough and the method is suitable for industrial application.

Description

technical field [0001] The invention relates to a method for extracting steviol glycosides. Background technique [0002] Stevia is a perennial herbaceous plant of Compositae, native to subtropical South America. The roots, stems and leaves of Stevia rebaudiana contain steviol glycosides. Stevioside has the characteristics of high sweetness (200-300 times that of sucrose), low calorie (only 1 / 300 of sucrose), and can increase blood sugar, lower blood pressure, and promote metabolism. Heart disease, dental caries, etc. have a certain auxiliary effect, so it has increasingly attracted people's attention and attention, and has become the "third sugar source" after sucrose and synthetic sugar. [0003] The planting area of ​​stevia is also expanding, and the supply exceeds the demand. Stevia products are widely used in medicine and food additive industries. At present, stevia leaves are mainly used in stevia rebaudiana, but the research on stevioside glycosides has disadvant...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
CPCC07H1/08C07H15/256
Inventor 贾德军
Owner MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP
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