Extraction technology for lava seawater containing dendrobium officinale

A technology of lava sea water and Dendrobium candidum, applied in skin care preparations, cosmetics, cosmetic preparations, etc., can solve the problems of reduced skin elasticity and low collagen synthesis, and achieve the effect of inhibiting saccharification and improving wrinkles

Active Publication Date: 2019-05-10
BIOLAND BIOCHEM HAIMEN CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the case of long-term exposure to ultraviolet rays, the active nutrients in the skin are produced in large quantities, leading to the promotion of the expression or activity

Method used

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  • Extraction technology for lava seawater containing dendrobium officinale
  • Extraction technology for lava seawater containing dendrobium officinale
  • Extraction technology for lava seawater containing dendrobium officinale

Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0023] Production example 1: Intake of lava sea water

[0024] The lava seawater collected at 44.35m below the average sea level (based on sea level) was sterilized with a sterilizing filter after digging 150m underground in Handong-ri, Gujo-eup, North Jeju-gun, in the eastern part of Jeju Island. The sterilized lava seawater (saline state without desalination) is used for the manufacture of Dendrobium officinale lava seawater extract in the future. The mineral composition of the sterilized lava seawater is shown in Table 1.

[0025] 【Table 1】

[0026]

Embodiment 1

[0027] Embodiment 1: the manufacture of dendrobium candidum lava seawater extract

[0028] Take 1 kg of Dendrobium candidum stems, add 20 kg of lava seawater, reflux extraction at 90°C for 3 hours, filter with a 250-mesh filter cloth, and then filter with a 1㎛ filter. The solvent was completely removed from the filtrate with a vacuum concentrator (EYELA사, N-1000, Japan), and 226 g of extract powder of Dendrobium officinale and lava seawater were obtained.

[0029] Adding 0.01-10% weight of Dendrobium candidum extract in lava seawater based on 100% weight of the whole has a good improvement effect on skin wrinkles and inhibition of saccharification, and has whitening and moisturizing effects.

experiment example 2

[0039] Experimental Example 2: Survey of Collagen type I mRNA expression

[0040] In order to measure the collagen synthesis promoting effect of the Dendrobium candidum lava seawater extract of Example 1 and the pure water extract of Dendrobium candidum of Comparative Example 1, on commercially purchased human fibroblasts, the treatment samples were subjected to Collagen type I genetic Gene expression experiments. In a 60mm dish together with IMDM (Iscove's Modified Dulbesco's Media) medium containing 10% bovine serum, human fibroblasts purchased from ATCC (Americal Type Culture Collection) were added at 1x10 6 Cells / well concentration dispensed at 37°C, 5% CO 2 Conditioned for 24 hours. After 24 hours, the samples were treated in IMDM culture medium without bovine serum and diluted according to the concentration for 24 hours, and the cells were collected in TRIzol (invitrogen, USA) to isolate RNA. Using Qubit to isolate RNA After quantification by fluoremeter with RNA B...

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Abstract

The invention discloses an extraction technology for lava seawater containing dendrobium officinale. The lava seawater is adopted as an extract stock solution and sterilized with a sterilization filter device; dendrobium officinale stems are added into the lava seawater, reflux extraction is conducted, then filtering is conducted with a filter cloth, then, a filter device is used for filtering until a solvent is completely removed, and finally dendrobium officinale lava seawater extract powder is obtained. According to the extraction technology, the lava seawater dendrobium officinale is adopted as an effective component to have the effects of removing wrinkles, restraining saccharifying, whitening and keeping moisture on skin.

Description

technical field [0001] The invention relates to a lava seawater extraction process related to dendrobium officinale. Background technique [0002] In the human body, the skin is the body organ that is most closely in contact with the external environment and has the function of protecting the inside of the human body. It can be divided into epidermis, dermis, and subcutaneous fat. The skin is a complex organ composed of cells with various functions and corresponding substances. The dermis is a combination of elastic solid substances (fibers) and viscous liquid substances, which can maintain the inherent elasticity of the skin. So the skin has a unique physical response to tension, which we call viscoelasticity. The reason why the skin has such viscoelasticity is that proteoglycans have collagen fibers and elastic fibers, which form a unique three-dimensional structure. Generally, due to aging, ultraviolet rays, pollution, stress and other reasons, skin function declines an...

Claims

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Application Information

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IPC IPC(8): A61K8/9794A61K8/96A61Q19/02A61Q19/08A61Q19/00
Inventor 金基洙金止焄卢勇金庚泰陈秀玉朴素延李昭憲史永昇金藝香
Owner BIOLAND BIOCHEM HAIMEN CO LTD
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