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Application of indole-propioponic acid to preparing medicine for controlling rheumatoid arthritis

A technology of indole propionic acid and rheumatoid, which is applied in the direction of drug combination, antipyretic, anti-inflammatory agent, etc., and can solve problems such as inability to effectively relieve diseases

Inactive Publication Date: 2019-05-10
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of hormones, immunosuppressants, and biological agents to suppress the immune response cannot achieve the purpose of effectively alleviating the disease

Method used

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  • Application of indole-propioponic acid to preparing medicine for controlling rheumatoid arthritis
  • Application of indole-propioponic acid to preparing medicine for controlling rheumatoid arthritis
  • Application of indole-propioponic acid to preparing medicine for controlling rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Significant reduction in serum indolepropionic acid levels in patients with rheumatoid arthritis

Embodiment approach

[0029] (1) Standard product configuration: an appropriate amount of IPA (sigma) powder was dissolved in methanol solution to prepare a storage solution with a concentration of 10 mmol / mL.

[0030] (2) Detecting the content of indole propionic acid by gas chromatography-mass spectrometry (GC-MS): collect the peripheral blood of rheumatoid arthritis patients (RA, n=60) and healthy control population (HC, n=40), Separate serum. Take about 70 μL into a 1.5mLEP tube, add 210 μL of acetonitrile solution, vortex for 45 seconds, centrifuge at 16000g / min for 10 minutes at 4°C, take 100ul of the supernatant, and inject 20μL. Refer to the IPA standard database, use PeakView1.2 software to characterize the IPA in the sample, use MultiQuant2.1 to quantify the qualitative IPA, and use the standard curve to calculate the corresponding concentration. see results figure 1 .

[0031] Research result

[0032] figure 1 The results showed that the content of indole propionic acid (IPA) in the...

Embodiment 2

[0034] Indolepropionic acid inhibits the development of experimental arthritis

[0035] Method of implementation

[0036](1) Constructing a collagen-induced joint (CIA) model: 1) Dissolving collagen, dissolving bovine type II collagen (CII) in 0.1mol glacial acetic acid, adjusting the final concentration of CII to 4mg / mL, and overnight at 4°C. 2) Emulsifying collagen, mixing completely dissolved CII with an equal volume of Freund's complete adjuvant (CFA) to fully emulsify the two to prepare a collagen emulsion with a final concentration of 2 mg / mL. 3) For the first immunization, the base of the tail of the mice was depilated, and a total of 200 μg of collagen emulsion was injected intradermally at multiple points. 4) Booster immunization. On the 21st day after the initial immunization, dissolve type II collagen in 0.1mol glacial acetic acid (concentration 4mg / mL), add an equal volume of incomplete Freund's adjuvant (IFA) to make collagen emulsion (final concentration 2mg / m...

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Abstract

The invention provides application of indole-propioponic acid to preparing medicine for controlling rheumatoid arthritis, and relates to the technical field of rheumatoid arthritis disease treatment.Through the indole-propioponic acid, occurrence and development of experimental arthritis can be remarkably inhibited, and joint swelling symptoms are effectively relieved. The indole-propioponic acidcan inhibit a proinflammatory cell Th1 and a follicular helper T cell (Tfh) and increase the proportion of an anti-inflammation T regulatory cell (T regulatory, Treg). The indole-propioponic acid canalso reduce expression of proinflammatory factors IL-1beta, IL-6 and IL-17A in serum and anti-CII (anti-type II collage antibody), and can increase secretion of beta-endorphin with the analgesic effect. The indole-propioponic acid has anti-inflammation and immunosuppression effects, the unbalance state of immune cells in organisms can be corrected, and the aim of controlling rheumatoid arthritisis thus achieved.

Description

technical field [0001] The invention relates to the technical field of treating rheumatoid arthritis diseases, in particular to the application of indole propionic acid in the preparation of drugs for preventing and treating rheumatoid arthritis. Background technique [0002] The lesions of rheumatoid arthritis mainly invade the joints, and the symptoms of the joints generally recur, and the damage of the joints becomes more and more serious, eventually leading to varying degrees of dysfunction and deformity. In addition to joints, skin rheumatoid nodules, arteritis, pericarditis, scleritis, lymphadenitis, hepatosplenomegaly, and neuropathy are not uncommon. The prevalence of rheumatoid arthritis in my country is 0.28-0.41%, with as many as 5 million patients, and the disability rate is as high as 75% without formal treatment. In the second national sampling survey of disabled people in my country in 2006, disabling joint disease ranked second (20.1%) among the 21 causes of...

Claims

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Application Information

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IPC IPC(8): A61K31/405A61P29/00A61P19/02
Inventor 栗占国李静靳家扬何菁贾汝琳肖文静
Owner PEOPLES HOSPITAL PEKING UNIV
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