A method and system for quantitatively adding reagents for biochips

A biochip and reagent technology, applied in the direction of measuring tube/pipette, etc., to achieve the effect of low cost, easy implementation, and high adding precision

Active Publication Date: 2019-07-30
HUNAN LEGEND AI CHIP BIOTECH CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, inside the biochip, reagent (sample) quantification cannot be achieved by adding an internal pump, and it is necessary to develop other new reagent quantification technologies that are simple to operate, easy to implement and inexpensive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method and system for quantitatively adding reagents for biochips
  • A method and system for quantitatively adding reagents for biochips
  • A method and system for quantitatively adding reagents for biochips

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] like figure 1 As shown, the methods for quantitatively adding reagents to the biochip include:

[0034] The displacement drive unit 3 drives the plunger 2 in the biochip reagent chamber 1 to move downward at a constant speed V1;

[0035] During the downward movement of the plunger 2 at a constant speed, calculate the total number of droplets dripping from the drip port of the reagent chamber 1; when the total number of droplets is equal to the preset value, the displacement drive unit 3 stops driving the plunger 2 downward Move; wherein, the preset value is determined by the following method:

[0036] Firstly, according to the dosage and properties of different kinds of reagents, the reagent chamber 1 and the drip port at the outlet are designed and manufactured through a combination of theoretical calculation and experimental verification, so as to obtain reagent droplets of a predetermined size. When the displacement drive unit 3 drives the plunger 2 in the reagent ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method and system for quantitatively adding reagents for biochips, wherein the method for quantitatively adding reagents comprises: a displacement drive unit drives a plunger in the reagent chamber of the biochip to move downward at a constant speed of V1; the plunger moves downward at a constant speed During the process, calculate the total number of droplets dripping from the drip port of the reagent chamber; when the total number of droplets is equal to the preset value, the displacement drive unit stops driving the plunger to move downward; when the total number of droplets is equal to the preset value When minus 1, the displacement driving unit drives the plunger to move downward at a constant speed of V2, where V2 is less than or equal to V1; before the plunger moves downward at a constant speed, the identification area is pre-calibrated under the reagent chamber drip port; During the downward movement, the image of the recognition area is acquired in real time, and it is judged whether there is a droplet dripping or not according to the image recognition result of the recognition area. The invention can precisely control the amount of reagent added into the reactor, has high precision in quantitative addition, has low requirements on the overall biochip, is low in cost, and is easy to implement.

Description

technical field [0001] The invention belongs to the field of biochip detection, in particular to a method and system for quantitatively adding reagents for biochips. Background technique [0002] Precise quantification of reagents (sample) is an important prerequisite for all automated analysis and testing instruments to output accurate results. At present, the automatic analysis instruments on the market realize the quantification of reagents (sample) generally through micro-quantitative needles, precision pumps or precision motor drives. The accuracy of reagent (sample) quantification will directly affect the reproducibility and accuracy of test results. The micro-quantitative needle itself is relatively small, precise and expensive. Generally, an instrument can only be equipped with a few; There is a risk of reagent (sample) cross-contamination. Realizing reagent (sample) quantification by means of precision motor control stroke requires high precision, high cost, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/02
Inventor 邬鹏程陈明黄常青
Owner HUNAN LEGEND AI CHIP BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap