Quantitative reagent addition method and quantitative reagent addition system for biological chip

Abstract

The invention discloses a quantitative reagent addition method and a quantitative reagent addition system for a biological chip. The quantitative reagent addition method comprises the steps of using adisplacement driving unit to drive a plunger in a biological chip reagent chamber to move downwards at a constant speed V1; when the plunger moves downwards at the constant speed, calculating the total amount of droplets dripping from a reagent chamber dripping port; when the total number of the droplets is equal to a preset value, enabling the displacement driving unit to stop driving the downward movement of the plunger; when the total number of the droplets is equal to a result of the preset value minus 1, using the displacement driving unit to drive the plunger to move downwards at a constant speed V2, wherein V2 is less than or equal to V1; before the plunger moves downwards at the constant speed, pre-calibrating an identification area under the reagent chamber dripping port; when the plunger moves downwards at the constant speed, acquiring the images of the identification area in real time, and determining whether the droplets drip or not according to the image recognition result of the identification area. The method and the system can accurately control the amount of reagents added into the reactor, and are high in precision of quantitative addition, low in requirements onthe overall biological chip, low in cost and is easy to implement.

Description

technical field [0001] The invention belongs to the field of biochip detection, in particular to a method and system for quantitatively adding reagents for biochips. Background technique [0002] Precise quantification of reagents (sample) is an important prerequisite for all automated analysis and testing instruments to output accurate results. At present, the automatic analysis instruments on the market realize the quantification of reagents (sample) generally through micro-quantitative needles, precision pumps or precision motor drives. The accuracy of reagent (sample) quantification will directly affect the reproducibility and accuracy of test results. The micro-quantitative needle itself is relatively small, precise and expensive. Generally, an instrument can only be equipped with a few; There is a risk of reagent (sample) cross-contamination. Realizing reagent (sample) quantification by means of precision motor control stroke requires high precision, high cost, and ...

AI Technical Summary

Problems solved by technology

Therefore, inside the biochip, reagent (sample) quantification cannot be achieved by adding an internal pump, and it is necessary to develop other new reagent quantification technologies that are simple to operate, easy to implement and inexpensive

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