Acinetobacter rhizosphaerae capable of efficiently removing phosphorus and application thereof

An Acinetobacter and Rhizobia technology, applied in the field of environmental microorganisms, can solve the problems of substandard sewage discharge and low phosphorus removal efficiency, and achieve the effects of improving phosphorus removal rate, improving effluent quality and great application prospects.

Inactive Publication Date: 2019-05-17
广州中大环境治理工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects and deficiencies such as low phosphorus removal efficiency and substandard sewage discharge in the existing biological phosphorus removal technology in sewage treatment.

Method used

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  • Acinetobacter rhizosphaerae capable of efficiently removing phosphorus and application thereof
  • Acinetobacter rhizosphaerae capable of efficiently removing phosphorus and application thereof
  • Acinetobacter rhizosphaerae capable of efficiently removing phosphorus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Screening, isolation and identification of embodiment 1 bacterial strain

[0039] 1. Source of the strain

[0040] Sediment of a section of the Xiaogu River in the East Campus of Sun Yat-Sen University, Guangzhou

[0041] 2. Medium

[0042] (1) Enrichment medium: 5.0 g of anhydrous sodium acetate, MgSO 4 ·7H 2 O 0.5g, CaCl 2 0.2g, (NH 4 ) 2 SO 4 2.0g, KH 2 PO 4 2~20mg, 2mL of trace element solution, sterile water to 1000mL, pH7.0~7.2, increase the KH in the medium successively 2 PO 4Content: 2, 5, 10, 15, 20mg.

[0043] (2) LB medium: peptone 10.0g, yeast extract powder 5.0g, glucose 1.0g, sodium chloride 5.0g, agar 15.0g, sterile water to 1000mL, pH7.0±0.2.

[0044] (3) Activation medium: peptone 10.0g, yeast extract powder 5.0g, glucose 1.0g, sodium chloride 5.0g, sterile water to 1000mL, pH7.0±0.2.

[0045] (4) Detection medium: CH 3 COONa 1g, MgSO 4 82mg, FeSO 4 ·7H 2 O 6.76mg, CaCl 2 60mg, (NH 4 ) 2 SO 4 0.2g, beef extract 0.22g, K 2 HPO 4...

Embodiment 2

[0055] The application of embodiment 2 phosphorus accumulating bacteria

[0056] 1. Phosphorus removal efficiency of phosphorus accumulating bacteria

[0057] Pick a loop of the phosphorus-removing bacteria obtained in Example 1 and insert it into the activation medium, and after cultivating it on a shaker at 30°C and 150r / min for 12h, transfer the bacterial suspension to 200mL detection medium at a ratio of 10%. . The cultivation process includes two processes of anaerobic and aerobic, so first seal the conical flask containing the mixed solution with a rubber stopper, and then pass in filtered and sterilized high-purity nitrogen to provide an anaerobic environment; anaerobic static After culturing for 24 hours, transfer it to a shaker at 30°C and 150r / min for aerobic cultivation for 24 hours; after the cultivation, take the culture solution and centrifuge it at 10000r / min for 10min, and take the supernatant to measure the total phosphorus concentration according to the moly...

Embodiment 3

[0062] Example 3 Effect of pH on Phosphorus Removal Efficiency of Bacteria

[0063] Adjust the detection medium pH value to be respectively 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, the bacterial strain obtained by screening in Example 1 is inoculated into 250mL of the medium with 200mL different pH values ​​by 10% inoculum In the Erlenmeyer flask, pass nitrogen into the flask to maintain the anaerobic condition. After 24 hours of static cultivation, cultivate it aerobically at 30°C and 150r / min for 24 hours, measure the concentration of total phosphorus in the final supernatant, and obtain the phosphorus removal rate . The result is as Figure 4 As shown, the growth conditions of rhizobia in different pH culture solutions are also different. As the pH value increases, the biomass first increases and then decreases. The OD600 value is the highest at pH=6 and pH=7, and the biomass is the largest. When the biomass of the culture medium is the largest, the removal of phosphorus reach...

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Abstract

The invention discloses Acinetobacter rhizosphaerae capable of efficiently removing phosphorus and application thereof, wherein the strain is preserved in the Guangdong Microbial Strain Culture Collection Center on September 28, 2017, and the culture preservation number of the strain is GDMCC NO:60246; the classification of the strain is names as Acinetobacter rhizosphaera; the preservation address is the fifth floor of the No.59 building of the N0.100 yard in Xianlie Central Road, Guangzhou, China. The strain has a good phosphorus removal effect, and the phosphorus removal rate can reach 95%at most after 24-hour anaerobic culture and 24-hour aerobic culture; when the strain is used for a biological phosphorus removal process, the phosphorus removal rate can be effectively increased, theeffluent quality is improved, and the strain has a great application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms. More specifically, it relates to a high-efficiency phosphorus removal Acinetobacter rhizobia and its application. Background technique [0002] In recent years, with the acceleration of urbanization and the rapid increase of urban population, the output of urban domestic sewage is increasing day by day. Sewage is rich in nutrients (especially nitrogen and phosphorus elements), poor treatment effect and substandard effluent will lead to severe eutrophication, manifested as algae blooms and water quality deterioration. Phosphorus is the key factor to control eutrophication. In recent years, the removal of phosphorus has attracted the attention of many scholars at home and abroad. [0003] At this stage, phosphorus removal technologies in sewage treatment plants mainly include biological phosphorus removal and chemical precipitation phosphorus removal. However, chemical method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 肖晶郭丽英赵永柯贤成梁咏梅李达徐梁刘伟
Owner 广州中大环境治理工程有限公司
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