Psychrobacter pulmonis strain T-15 for producing polysaccharide and application of strain

A technology of psychrophilic bacillus and T-15, which is applied in the field of microorganisms, can solve the problems of less endophytic bacteria in Ulva, and achieve the effects of good antioxidant activity, low fermentation cost and high economic benefit.

Inactive Publication Date: 2019-05-21
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

[0004] The endophytes of Brasenia have only begun to attract people's attention in recent years. Generally speaking, there are relatively few studies on the endophytes of Brasenia, especially those that can metabolize the corresponding polysaccharide components of Brasenia, and there are few studies on the endophytes of Brasenia that produce polysaccharides. report

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  • Psychrobacter pulmonis strain T-15 for producing polysaccharide and application of strain
  • Psychrobacter pulmonis strain T-15 for producing polysaccharide and application of strain
  • Psychrobacter pulmonis strain T-15 for producing polysaccharide and application of strain

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Embodiment Construction

[0014] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0015] Isolation of Endophytic Bacteria from Brasenia officinalis

[0016] Take the stems, leaves and roots of fresh water shield, sterilize them with mercuric chloride and rinse them with distilled water several times in a sterile environment, inoculate them on LB solid medium, and culture them in the dark. Well-growing colonies were picked from the culture medium and transferred to new LB medium plates, purified and numbered respectively. The bacterial strain provided in the present embodiment is named No. T-15 bacterial strain, and the bacterial colony of this bacterial strain is moved to the new LB culture medium plate and grows well, and its colony morphology figure is as follows figure 1 as shown, figure 1 Middle scale bar = 2mm. From figure 1 It can be seen that the diamete...

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Abstract

The invention provides a T-15 strain for producing polysaccharide and belongs to a psychrobacter pulmonis strain. The strain is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the postcode is 430072, the preservation date is January seventeenth, 2019, and the preservation number is CCTCC M 2019056; the 16S rNDA sequence of the strain is shown in SEQ ID NO.1. The psychrobacter pulmonis T-15 strain can be applied to polysaccharide preparation through fermentation. The strain can be cultured through fermentation in large scale in short time, the fermentation cost is low, fermentation is not limited by conditions such as territory and season, the current condition of increasingly scarce natural resources of brasenia schreberi can be solvedthrough a microbial fermentation way, and accordingly the economic benefit is high.

Description

technical field [0001] The invention relates to a strain of psychrophilic bacillus pulmonary apex, which can produce polysaccharides and belongs to the technical field of microbes. Background technique [0002] At present, the research on plant endophytes has been paid more and more attention, and various endophytes of different plants have been discovered one after another. The diversity of endophyte species also endows the diversity of its metabolites, which also indicates that the use of microbial metabolism to discover new resources such as biology, chemistry, and medicine has great potential. A large number of studies have shown that plant endophytes can produce the same or similar active substances and precursors as those of host plants. This discovery directly promotes the extensive research on plant endophytes, especially economical plant endophytes. With people's gradual research on economical plant endophytes, the use of endophytic bacteria that can metabolize and...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
Inventor 余光辉凡筱芃覃瑞龚汉雨
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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