Method for screening and measuring 54 kinds of pesticide residues in onion, ginger and garlic by gas chromatography-tandem mass spectrometry
A technology with multiple residues of pesticides and tandem mass spectrometry, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex matrix and difficult sample processing, and achieve the effect of less operation steps, more test items and good accuracy
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specific Embodiment approach 1
[0023] Specific embodiment one: a kind of adopting gas chromatography tandem mass spectrometry screening of the present embodiment and the method for 54 kinds of pesticide residues in green onion, ginger, garlic, it is carried out according to the following steps:
[0024] 1. Cut the onion, ginger and garlic into pieces, put them into a wall breaker or a high-speed tissue grinder and mix them evenly, and then seal them as samples;
[0025] 2. Place the sample in step 1 in a centrifuge tube, add 35% water / acetonitrile solution by volume, perform homogenate extraction for 10 minutes, centrifuge, take the supernatant, and obtain the solution to be purified;
[0026] 3. Purification treatment:
[0027] (1) Take the above liquid to be purified, add an equal volume of saturated sodium chloride solution, and add deionized water at a volume ratio of 2.5:6;
[0028] (2) Connect the DPX tip-type dispersive solid-phase microextraction column, add 1 mL of acetonitrile from the upper end ...
specific Embodiment approach 2
[0035] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the chromatographic conditions in gas chromatography-tandem mass spectrometry are:
[0036] Chromatographic column: J&W HP-5ms Ultra Inert fused silica capillary column; 30m×0.25mm×0.25μm;
[0037] Heating program: keep at 60°C for 1min, rise to 170°C at 40°C / min, then rise to 310°C at 10°C / min and hold for 3min;
[0038] Injection volume: 1μL;
[0039] Carrier gas: helium, purity ≥99.999%;
[0040] Flow rate: 0.8mL / min;
[0041] Injection port temperature: 250°C;
[0042] Injection method: splitless injection.
[0043] Others are the same as in the first embodiment.
specific Embodiment approach 3
[0044] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the mass spectrometry conditions in gas chromatography-tandem mass spectrometry are:
[0045] Using electron bombardment ion source;
[0046] Electron energy: 70eV;
[0047] Ion source temperature: 300°C;
[0048] Solvent delay time: 4.08min;
[0049] Scanning mode: multiple reaction ion monitoring mode.
[0050] Others are the same as in the first embodiment.
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