Method for screening and measuring 54 kinds of pesticide residues in onion, ginger and garlic by gas chromatography-tandem mass spectrometry

A technology with multiple residues of pesticides and tandem mass spectrometry, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex matrix and difficult sample processing, and achieve the effect of less operation steps, more test items and good accuracy

Inactive Publication Date: 2019-05-21
无锡微色谱生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the pretreatment of samples is difficult due to the complexity of onion, ginger, and garlic substrates in the current screening of pesticide residues, and the volatile oil components such as allium, and to provide a method that uses gas chromatography in series Mass Spectrometry Screening and Determination of 54 Pesticide Residues in Onion, Ginger and Garlic

Method used

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  • Method for screening and measuring 54 kinds of pesticide residues in onion, ginger and garlic by gas chromatography-tandem mass spectrometry
  • Method for screening and measuring 54 kinds of pesticide residues in onion, ginger and garlic by gas chromatography-tandem mass spectrometry
  • Method for screening and measuring 54 kinds of pesticide residues in onion, ginger and garlic by gas chromatography-tandem mass spectrometry

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specific Embodiment approach 1

[0023] Specific embodiment one: a kind of adopting gas chromatography tandem mass spectrometry screening of the present embodiment and the method for 54 kinds of pesticide residues in green onion, ginger, garlic, it is carried out according to the following steps:

[0024] 1. Cut the onion, ginger and garlic into pieces, put them into a wall breaker or a high-speed tissue grinder and mix them evenly, and then seal them as samples;

[0025] 2. Place the sample in step 1 in a centrifuge tube, add 35% water / acetonitrile solution by volume, perform homogenate extraction for 10 minutes, centrifuge, take the supernatant, and obtain the solution to be purified;

[0026] 3. Purification treatment:

[0027] (1) Take the above liquid to be purified, add an equal volume of saturated sodium chloride solution, and add deionized water at a volume ratio of 2.5:6;

[0028] (2) Connect the DPX tip-type dispersive solid-phase microextraction column, add 1 mL of acetonitrile from the upper end ...

specific Embodiment approach 2

[0035] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the chromatographic conditions in gas chromatography-tandem mass spectrometry are:

[0036] Chromatographic column: J&W HP-5ms Ultra Inert fused silica capillary column; 30m×0.25mm×0.25μm;

[0037] Heating program: keep at 60°C for 1min, rise to 170°C at 40°C / min, then rise to 310°C at 10°C / min and hold for 3min;

[0038] Injection volume: 1μL;

[0039] Carrier gas: helium, purity ≥99.999%;

[0040] Flow rate: 0.8mL / min;

[0041] Injection port temperature: 250°C;

[0042] Injection method: splitless injection.

[0043] Others are the same as in the first embodiment.

specific Embodiment approach 3

[0044] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the mass spectrometry conditions in gas chromatography-tandem mass spectrometry are:

[0045] Using electron bombardment ion source;

[0046] Electron energy: 70eV;

[0047] Ion source temperature: 300°C;

[0048] Solvent delay time: 4.08min;

[0049] Scanning mode: multiple reaction ion monitoring mode.

[0050] Others are the same as in the first embodiment.

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Abstract

Provided in the invention is a method for screening and measuring 54 kinds of pesticide residues in onion, ginger and garlic by gas chromatography-tandem mass spectrometry. The invention relates to amethod for screening and measuring 54 kinds of pesticide residues in onion, ginger and garlic. Therefore, a problem that pre treatment of the sample becomes difficult in the existing pesticide residuescreening and measuring because the ground substances of the onion, ginger and garlic are complicated and contain lots of interfering substances is solved. A DPX spearhead type dispersed solid phasemicro-extraction column is established and the residual amounts of 54 kinds of pesticides in green onion, ginger and garlic are measured by combining GC-MS/MS. The DPX method has a good purification effect and a few of operation steps; and no interference is caused on the detection result by the ground substances. The method having advantages of many test items, simplenness and rapidness, high accuracy and flux is suitable for screening and detecting the pesticides in the onion, ginger and garlic. The method is applied to the field of pesticide detection.

Description

technical field [0001] The invention relates to a method for screening and measuring 54 kinds of pesticide residues in green onions, ginger and garlic. Background technique [0002] Onion, ginger, and garlic are traditional table spices in my country. They have unique flavor and rich nutrition. They have a strong effect of sterilizing or inhibiting bacteria and viruses. , anti-cancer and other health functions, so it is deeply loved by the public, and it is also one of my country's important export agricultural products. In the planting process of green onions, ginger, and garlic, the use of pesticides is unavoidable in order to prevent pests and diseases. However, if the use of pesticides is not standardized, it will often lead to excessive pesticide residues. In addition, exogenous pollution such as air, soil and water is also an important reason for excessive pesticide residues. Excessive pesticide residues not only endanger human health, but also affect the competitive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/08G01N30/72
Inventor 杨佳卡莫莱费静严璐冯玥王倩
Owner 无锡微色谱生物科技有限公司
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