A kind of stable pickering emulsion based on black tea nano-aggregate and its preparation method and application
An aggregate, dark tea technology, applied in the field of materials, can solve problems such as restricting applications, and achieve the effect of broadening the application field and improving the application value
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Embodiment 1
[0029]Example 1: Construction of black tea nano aggregate pickering emulsion.
[0030](1) Accurately weigh 4.5g black tea tea in a 250ml conical bottle, add 25 ml of boiling water, 30 min, oscillated once every 10 minutes, heat filter, cool.
[0031](2) Take 4 ml of black tea extract in the 100 kDa ultrafiltration tube, centrifuge at a temperature of 4 ° C and 4000 g of centrifugation at 4000 g of centrifugation; the internal / outer tube solution is collected (an average diameter> 10 nm and an average diameter ≤10 nm The nano-aggregate solution, freeze-dried powder, that is, black tea nano aggregate, is for use.
[0032](3) Fixed black tea nano aggregate addition is 2% mass, ultrapure water dissolved, add 0.02% of mass fraction, 1 mol / L HCl or 1 mol / L NaOH to adjust black tea water extract The pH is 8, the tin foil paper is protected from light overnight (12 ~ 14h), the volume ratio of oil and water is 4: 6 to add soybean oil (total volume 50ml), 20000R / min shear homogeneous 2min, 40M...
Embodiment 2
[0033]Example 2: Determination of emulsion droplets and particle size distribution
[0034]The particle size and particle size distribution of the fresh or reserved emulsion prepared by the present invention utilizes the Marvin 3000 laser particle size gauge, and deionized water and 1% SDS are used as the dispersant. The relative refractive coefficient of the emulsion is set to 1.095, the ratio of the soybean oil refractive coefficient (1.456) and the water refractive coefficient (1.33). The particle size of the emulsion is represented by D4, 3 (volume weighted average particle diameter). The results show: black tea nano aggregate (average diameter>10nm) Pickering emulsion droplet diameter is 2.93 ± 0.01 μm, mainly in small particle distribution; black tea nano aggregate (an average diameter ≤10nm) Pickering emulsion droplet diameter is 7.00 ± 0.06 μm, mainly particle distribution . Black tea nano aggregate (average diameter>10 nm) emulsion droplets particle size distribution compared ...
Embodiment 3
[0035]Example 3: Determination of electrostatic stability of emulsion system
[0036]The emulsion Zeta potential was determined by LDV technology to study the static stability of the Pickering emulsion based on black tea nano aggregate stabilization. The results show: Black tea nano aggregate (average diameter>10nm) Pickering emulsion system Zeta potential value of 52.0 ± 1.7mV, black tea nano aggregate (an average diameter ≤10nm) Pickering emulsion system Zeta potential value 61.9 ± 0.7mV. Black tea nano aggregate (average diameter>10 nm and average diameter ≤10nm) The Pickering emulsion system Zeta potential value is greater than 50 mV, and the stability of the system is high.
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