Method for evaluating demethyltransferase activity of histone lysine

A histone and lysine technology, applied in the field of enzyme activity detection, can solve the problem of inability to monitor the enzyme reaction in real time

Active Publication Date: 2019-05-31
SHENZHEN UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Benefits of technology

In summary, it describes how we use certain chemicals called carboxylic acids or bases (CA) with specific properties that help us detect changes caused by DNA damage during various processes like gene expression regulation. By measuring these modifications, researchers are able to better identify which parts of cells have been damaged due to exposure to harmful agents. These techniques could lead to new ways for developing drugs targeting those areas affected.

Problems solved by technology

This patented technical solution described in the patents relates to monitoring changes made during cellular processes like DNA synthesis, protein folding, oxidative stress response, metabolism, etc., particularly how certain chemical substances called histone dimers may affect specific parts of genes involved in important functions within normal physiology. By measuring its modification levels overcomes previous limitations associated with current techniques involving analyzing each step separately from other steps.

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  • Method for evaluating demethyltransferase activity of histone lysine
  • Method for evaluating demethyltransferase activity of histone lysine
  • Method for evaluating demethyltransferase activity of histone lysine

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Embodiment Construction

[0019] The present invention provides a method for evaluating the activity of histone lysine demethyltransferase containing Jumonji-C domain. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0020] An embodiment of the present invention provides a method for evaluating the activity of a histone lysine demethyltransferase containing a Jumonji-C domain, wherein carbon-13-labeled α-KG and a nuclear magnetic resonance spectrometer are used in a biological mixture (such as cell lysis Selective real-time monitoring of the changes of substrate α-KG and product succinate of histone lysine demethyltransferase containing Jumonji-C domain in liquid, etc.) to evaluate histone lysine demethylation transfer enzyme activi...

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Abstract

The invention discloses a method for evaluating the demethyltransferase activity of histone lysine. The method utilizes isotope labeled alpha-ketoglutaric acid and a nuclear magnetic resonance spectroscope to selectively monitor the change, in biological mixture, a substrate alpha-ketoglutaric acid and a product succinic acid of histone lysine demethyltransferase containing a Jumonji-C structure domain in real time so as to assess the demethyltransferase activity of the histone lysine. The method can determine enzyme activity and can be applied to the development of enzyme inhibitors.

Description

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Claims

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Application Information

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Owner SHENZHEN UNIV
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