Method for evaluating demethyltransferase activity of histone lysine

Abstract

The invention discloses a method for evaluating the demethyltransferase activity of histone lysine. The method utilizes isotope labeled alpha-ketoglutaric acid and a nuclear magnetic resonance spectroscope to selectively monitor the change, in biological mixture, a substrate alpha-ketoglutaric acid and a product succinic acid of histone lysine demethyltransferase containing a Jumonji-C structure domain in real time so as to assess the demethyltransferase activity of the histone lysine. The method can determine enzyme activity and can be applied to the development of enzyme inhibitors.

Description

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AI Technical Summary

Benefits of technology

In summary, it describes how we use certain chemicals called carboxylic acids or bases (CA) with specific properties that help us detect changes caused by DNA damage during various processes like gene expression regulation. By measuring these modifications, researchers are able to better identify which parts of cells have been damaged due to exposure to harmful agents. These techniques could lead to new ways for developing drugs targeting those areas affected.

Problems solved by technology

This patented technical solution described in the patents relates to monitoring changes made during cellular processes like DNA synthesis, protein folding, oxidative stress response, metabolism, etc., particularly how certain chemical substances called histone dimers may affect specific parts of genes involved in important functions within normal physiology. By measuring its modification levels overcomes previous limitations associated with current techniques involving analyzing each step separately from other steps.

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