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A kind of injectable silk fibroin porous hydrogel and preparation method thereof

A porous hydrogel and silk fibroin technology, which is applied in the field of injectable silk fibroin porous hydrogel and its preparation, can solve the problems of mechanical properties and limitations of preparation methods, and achieve adjustable curing speed and good biocompatibility , good mechanical properties

Active Publication Date: 2021-10-29
江西丝科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current silk fibroin hydrogels have many limitations in terms of mechanical properties and preparation methods. It is still a technology in this field to obtain injectable silk fibroin hydrogels without cross-linking agents, with excellent mechanical properties and porous structure. difficulty

Method used

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  • A kind of injectable silk fibroin porous hydrogel and preparation method thereof
  • A kind of injectable silk fibroin porous hydrogel and preparation method thereof
  • A kind of injectable silk fibroin porous hydrogel and preparation method thereof

Examples

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Effect test

Embodiment 1

[0051] This example is the silk fibroin porous hydrogel prepared by the preparation method of the present invention.

[0052] The preparation method of silk fibroin porous hydrogel described in the present embodiment is:

[0053] (1) Prepare silk fibroin aqueous solution, the schematic diagram is as follows figure 1 shown;

[0054] (2) Pour the silk fibroin aqueous solution into the dialysis bag, control the volatilization rate of water to concentrate, and obtain the silk fibroin concentrate, the schematic diagram is as follows figure 2 As shown; the specification of the dialysis bag is 10Da, the ambient temperature during concentration is 0°C, the humidity is 10%, and the concentration of the silk fibroin concentrate is 20%;

[0055] (3) Stir and shear the silk fibroin concentrate to make it pre-crosslinked to obtain silk fibroin fluid foam. The stirring speed is 1r / min, and the stirring time is 30s. The schematic diagram is as follows image 3 shown;

[0056] (4) Inject...

Embodiment 2

[0065] This example is the silk fibroin porous hydrogel prepared by the preparation method of the present invention.

[0066] The preparation method of silk fibroin porous hydrogel described in the present embodiment is:

[0067] (1) preparing silk fibroin aqueous solution;

[0068] (2) Pour the silk fibroin aqueous solution into the dialysis bag, control the volatilization rate of water to concentrate, and obtain the silk fibroin concentrate; The concentration of plain protein concentrate is 30%;

[0069] (3) stirring and shearing the silk fibroin concentrate to make it pre-crosslinked to obtain a silk fibroin fluid foam, the stirring speed is 200r / min, and the stirring time is 600s;

[0070] (4) Inject the silk fibroin fluid foam into the mold, and then obtain the injectable silk fibroin porous hydrogel after standing for 5 hours.

[0071] Wherein, the concrete preparation process of silk fibroin aqueous solution is as follows:

[0072] (a) heating silkworm cocoons in so...

Embodiment 3

[0079] This example is the silk fibroin porous hydrogel prepared by the preparation method of the present invention.

[0080] The preparation method of silk fibroin porous hydrogel described in the present embodiment is:

[0081] (1) preparing silk fibroin aqueous solution;

[0082] (2) Pour the silk fibroin aqueous solution into the dialysis bag, control the volatilization rate of water to concentrate, and obtain the silk fibroin concentrate; The concentration of plain protein concentrate is 25%;

[0083] (3) stirring and shearing the silk fibroin concentrate to make it pre-crosslinked to obtain a silk fibroin fluid foam, the stirring speed is 100r / min, and the stirring time is 300s;

[0084] (4) The silk fibroin fluid foam is injected into the mold, and the injectable silk fibroin porous hydrogel is obtained after standing for 2 hours.

[0085] Wherein, the concrete preparation process of silk fibroin aqueous solution is as follows:

[0086] (a) heating silkworm cocoons ...

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Abstract

The invention relates to an injectable silk fibroin porous hydrogel and a preparation method thereof, belonging to the technical field of organic polymer materials. The preparation method of the injectable silk fibroin porous hydrogel of the present invention comprises the following steps: preparing an aqueous silk fibroin solution; concentrating the silk fibroin aqueous solution to obtain a silk fibroin concentrate; stirring and shearing the silk fibroin concentrate to make it The silk fibroin fluid foam is obtained by pre-crosslinking; the silk fibroin fluid foam is injected into a mold, and the silk fibroin fluid foam is obtained after standing still. The preparation method of the present invention simulates the forming process of natural silk without adding any cross-linking agent, and the operation is simple; the prepared hydrogel can fill defects of any shape, meet the complex shapes of different wounds, and reduce the impact of implants on body tissues. Invasive; before injection, it can also be mixed with specific drugs and biologically active substances to form a hydrogel drug sustained release system.

Description

technical field [0001] The invention relates to an injectable silk fibroin porous hydrogel and a preparation method thereof, belonging to the technical field of organic polymer materials. Background technique [0002] At present, research on medical hydrogels has aroused widespread interest. Hydrogel materials have a variety of biological applications, including drug-controlled release carriers and tissue engineering scaffolds, etc., to repair defective tissues and organs. For tissue engineering scaffolds, they can be divided into preformed porous scaffolds and injectable materials according to their molding characteristics. The former is a three-dimensional porous cell scaffold with a specific shape and a certain pore structure, which is similar to the tissue or organ defect to be repaired, made in vitro by biomaterials, and then implanted with seed cells to construct the tissue, and then through surgery. The method of implanting this preformed system into the defect site ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08J9/00C08L89/00A61K9/06A61K47/42A61L27/22A61L27/52A61L27/54A61L27/56
Inventor 陶虎柳克银
Owner 江西丝科生物科技有限公司
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