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Total protein measurement using whole blood refractometry

A protein, refractive index technology used in the field of clinical analyzers to solve problems such as reducing accuracy

Active Publication Date: 2022-05-17
INSTR LAB
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Problems solved by technology

Light scattering by cells in whole blood significantly reduces the accuracy of refractive index measurements without compensation or optical assessment of cell-depleted plasma

Method used

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  • Total protein measurement using whole blood refractometry
  • Total protein measurement using whole blood refractometry
  • Total protein measurement using whole blood refractometry

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Embodiment Construction

[0016] Aspects of the present disclosure include methods and apparatus for performing refractometry on a substantially cell-free plasma layer adjacent to a channel wall of a channel containing a flowing whole blood sample. This allows the measurement of the total protein content of the sample without the additional step of isolating plasma from whole blood samples.

[0017] When a whole blood sample flows through a channel with a small cross-sectional diameter, such as a blood vessel in the body or a capillary on a chip, for example, the sample appears as a flow with a substantially cell-free plasma membrane present at the edge of the channel. The substantially cell-free plasma membrane is a very thin layer with a thickness of less than 1 micron to several microns at the edges of the channels. It is believed that the presence of substantially cell-free plasma membranes in blood vessels, for example, helps prevent blockages and reduces fluid resistance in small blood vessels in...

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Abstract

Optical systems and methods for the quantification of total protein in whole blood or other multiphase liquids and colloidal suspensions using refractometry without the need for preparatory steps such as cell separation or centrifugation. A refractometer is integrated with the flow cell to enable the measurement of the refractive index of a flowing sample based on the substantially cell-free boundary layer of the sample present under certain flow conditions. The dimensions of the flow cell are chosen to produce a cell-free layer in the whole blood flow, wherein the cell-free layer is thick enough to reduce scattering of light from the light source of the refractometer. Numerical methods are used to compensate for scattering artifacts. The numerical compensation method is based on the slope and width of the peaks in the derivative curve of the angular spectral image of the flowing sample produced by refractometry.

Description

technical field [0001] Aspects of the present disclosure relate to the field of clinical analyzers, and more particularly, to methods and devices for measuring total protein in plasma without separating the plasma from a whole blood sample. Background technique [0002] In various clinical situations it is important to measure certain chemical characteristics of plasma in whole blood samples. For example, it is often important to measure analytes in plasma, extracellular hemoglobin, bilirubin, and lipid particles. These situations include, for example, routine patient visits to a doctor's office, emergency room, or monitoring of inpatients. A number of techniques and devices are commonly used to measure chemical characteristics of bodily fluids in various clinical situations. For example, measurement of analytes in bodily fluid samples can be accomplished by various methods such as spectrometry and refractometry. [0003] Some techniques for analyzing bodily fluids are co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/27G01N33/68
CPCG01N21/27G01N33/6803G01N33/4915G01N21/4133
Inventor 伊森·舍恩布伦劳拉·阿迪布格特·布兰肯施泰因
Owner INSTR LAB
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