Quantitative research method for Dong e e-jiao, Fupai e-jiao and deer-horn gelatin

Abstract

The invention provides a quantitative research method for Dong e e-jiao, Fupai e-jiao and deer-horn gelatin, which relates to the technical field of e jiao quantitative research. The quantitative research method for Dong e e-jiao, Fupai e-jiao and deer-horn gelatin comprises the following steps: S1, firstly, an MSC spectrum preprocessing algorithm is established; S2, a BP-ANN modeling method is established; S3, samples are prepared, wherein the samples comprise 24 batches of samples of Dong e e-jiao, Fupai e-jiao and deer-horn gelatin; and S4, the sample is placed in a sample cup, the sample cup is ensured to be covered with the sample, an original spectrum of the sample is obtained by using a GSA102 type near-infrared spectrometer, and pretreatment is carried out by using an MSC algorithm. The MSC method can effectively eliminate the scattering influence caused by uneven particle distribution and particle size, and is especially suitable for powder near infrared spectrum pretreatment;the BP-ANN has the capabilities of autonomous learning and parallel processing, and the modeling method is relatively high in prediction accuracy.

Description

technical field [0001] The invention relates to the technical field of quantitative research on donkey-hide gelatin, in particular to a method for quantitative research on donkey-hide donkey-hide gelatin, fujiao and antler gum. Background technique [0002] The main component of donkey-hide gelatin is donkey skin, which has the functions of nourishing blood, nourishing yin, moistening dryness, and stopping bleeding. The main component of donkey skin is collagen, and the amino acid composition of collagen is relatively simple, while the amino acid similarity between fake collagen and donkey skin collagen Up to 90%, it is difficult to identify after being processed into donkey-hide gelatin, which seriously affects the curative effect and clinical drug safety of donkey-hide gelatin. [0003] At present, the existing quality evaluation methods of donkey-hide gelatin include electrophoresis method, HPLC method, amino acid fingerprint and peptide map method, and molecular identifi...

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Problems solved by technology

[0002] The main component of donkey-hide gelatin is donkey skin, which has the functions of nourishing blood, nourishing yin, moistening dryness, and stopping bleeding. The main component of donkey skin is collagen, and the amino acid composition of collagen is relatively simple, while the amino acid similarity between fake collagen and donkey skin collagen up to 90%, it is difficult to identify after being processed into donkey-hide gelatin, which seriously affects the curative effect of donkey-hide gelatin and the safety of clinical medication

[0003] At present, the existing quality evaluation methods of donkey-hide gelatin include electrophoresis method, HPLC method, amino acid fingerprint and peptide map method, and molecular identification method. Therefore, this method is difficult to achieve standardization. The amino acid composition of isotype collagens from different animal sources has a similarity of 90%. Therefore, the quality evaluation of HPLC method has certain limitations. Amino acid fingerprinting, peptide mapping and molecular identification methods are At present, the use method of quality evaluation is more feasible, but these two methods still need to be further improved in terms of standardization. In recent years, the research on the quality control method and technology of Ejiao has become difficult and hot. Quantitative analysis method of donkey-hide gelatin

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