Method for analyzing nmr spectra of lipoprotein-containing samples

A lipoprotein and lipoprotein particle technology, applied in the field of NMR spectrum analysis of lipoprotein-containing samples, can solve complex deconvolution and other problems

Active Publication Date: 2021-09-10
NUMARES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these signals require very complex deconvolution as they are present in all lipids and for that matter all lipoprotein classes and lipoprotein subclasses

Method used

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  • Method for analyzing nmr spectra of lipoprotein-containing samples
  • Method for analyzing nmr spectra of lipoprotein-containing samples
  • Method for analyzing nmr spectra of lipoprotein-containing samples

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Embodiment Construction

[0047] Figure 1A Shows an NMR spectrum of serum samples (black line). To subtracted NMR signals from all lipoprotein deficient serum (LPDS), and records the corresponding 2 LPDS NMR spectrum (gray line) and subtracting 1 from the spectrum in the serum. 3 also depicts the difference spectrum obtained in Figure 1A In (dashed line). The resulting difference in spectrum 3, can be seen not only from the lack of NMR signal component present in the serum lipoproteins. These are basically just lipoproteins and small metabolites. therefore, Figure 1A It depicts the standard 1 H NMR spectrum. In this and all the figures are depicted at the gray bar, gray bars indicate the observed spectral range wherein the signal generated by the solvent (water or organic solvent), in the solvent to dissolve each sample has been recorded NMR spectrum appropriate.

[0048] Figure 1B Showing Figure 1A Diffusion weighted NMR spectrum of the same sample. Thus, any superimposed signals from the metabolite in ...

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Abstract

The present invention relates to a method for analyzing the NMR spectrum of a lipoprotein-containing sample, comprising the steps of: a) limiting the spectral range to be analyzed to at least 0.5 ppm of the NMR spectrum (16) of the lipoprotein-containing sample, b) dividing the first A first spectral function (21) of an individual lipid component and at least one further spectral function (22) of an additional individual lipid component are fitted to the full spectral range to be analyzed, wherein said first spectral function ( 21) representing at least a portion of the NMR spectrum of said first individual lipid fraction, which takes into account the behavior of the first lipid fraction in the environment surrounding lipoprotein particles of the first defined lipoprotein class or lipoprotein subclass; Wherein said further spectral function (22) represents at least a part of the NMR spectrum of said further individual lipid component, which takes into account said Behavior of the additional lipid component; determining the concentration or amount of said first individual lipid component and said additional individual lipid component in the lipoprotein-containing sample based on the fitting operation of step b) and thereby determining The concentration or amount of said first defined lipoprotein class or lipoprotein subclass, and optionally the concentration or amount of each other defined lipoprotein class or lipoprotein subclass.

Description

[0001] manual Technical field [0002] The present invention relates to a method for NMR analysis of lipoprotein-containing samples of the spectrum. Background technique [0003] Methods known in the prior art for the analysis of the NMR spectrum, usually comprising a single wire or a combination Hill (hill) fitted to the NMR spectrum of the sample a small amount of a separate line fit function to separate the sample assigned to the concentration of the individual components of the measured NMR spectrum. To reduce the complexity of those fit function, usually fit only specific spectral region, for example, CH 2 CH 3 Area. [0004] To determine the concentration of lipoproteins or lipoprotein subclass must provide the appropriate reference spectrum or reference model (the reference value). Presupposes and then quantitatively analyzed with reference to the referenced class or subclass of lipoprotein particles to produce a defined reference lipoproteins or lipoprotein subclass refere...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01R33/465G01N24/08
CPCG01N24/08G01R33/4625G01R33/465
Inventor 达妮埃拉·鲍姆施塔克
Owner NUMARES
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