Network dynamic researching method for lipid metabolism

A lipid metabolism and lipid technology, applied in the fields of analytical chemistry and biochemistry, can solve the problems of lack of non-targeting strategies and achieve the effect of wide coverage, good repeatability and high molecular specificity

Active Publication Date: 2014-09-24
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

However, so far, these studies only focus on a certain type or several types of lipids, lacking a non-targeting strategy that can detect and cover a

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  • Network dynamic researching method for lipid metabolism
  • Network dynamic researching method for lipid metabolism

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Embodiment 1

[0033] Palmitate has been reported to induce cellular stress, insulin resistance, and induce apoptosis in skeletal muscle cells. However, the underlying lipid metabolism in the context of this study is unclear. Using stable isotope labeling-assisted lipidomics to study the metabolic trajectory of palmitic acid, downstream lipid metabolism and its dynamic process can provide new ideas for research in this area.

[0034] Human primary skeletal muscle myotube cells were cultured with EMEM medium (Eagle's minimum essential medium, minimum essential medium) containing 250 μM [U- 13 C]-palmitate, collect cells after 4h, 12h, 24h treatment time. [U- 13 C]-palmitate was previously incubated with BSA (bovine serum albumin) to 13 C]-palmitate binds to BSA ([U- 13 C]-palmitate:BSA=4:1, molar ratio) (Reference 3: Peter, A.; Weigert, C.; Staiger, H.; Machicao, F.; Schick, F.; Machann, J.; Stefan , N.; Thamer, C.; H.-U.; Schleicher, E. Diabetes 2009, 58, 1757–1765.). At the same tim...

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Abstract

The invention discloses a network dynamic searching method for lipid metabolism. The method is based on stable isotope labeling assisted lipidomics and combined with non-target isotope isomer screening. The network dynamic searching method is characterized in that stable isotope labeling cell, animal or human experiments, high resolution UHPLC-MS lipidomics analysis and the non-target isotope isomer screening are combined; the dynamic metabolism processes, such as synthesis, conversion and decomposition of lipid molecules in the lipid metabolism fingerprint acquisition can be captured while the lipid metabolism fingerprint in a static state is acquired. The network dynamic searching method for the lipid metabolism has high molecule specificity and extensive network coverage capability; the method is repeatable, steady and reliable. The method develops a new idea for deeply searching lipid metabolism and aims to gain more profound and comprehensive understanding in pathological process, physiological process and metabolism perturbation in the biosystem.

Description

technical field [0001] The invention relates to the fields of analytical chemistry and biochemistry, and is a new method for dynamic research of lipid metabolism network based on stable isotope label-assisted lipidomics combined with non-targeted isotopomer screening. Background technique [0002] Lipid is an important class of metabolites. In addition to storing energy, it is an important component of cell membrane structure and a key factor in regulating various signaling processes. Disorders of lipid metabolism are related to the occurrence and development of various diseases. Lipidomics is a powerful method to study lipid metabolism. However, most lipidomics studies can only obtain "snapshot" static lipid profiles, and cannot conduct in-depth studies on the dynamic processes of lipid metabolism (such as synthesis, transformation, degradation, etc.). [0003] The dynamic process of lipid metabolism can be captured by introducing stable isotope-labeled precursor molecule...

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Application Information

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IPC IPC(8): G06F19/00
Inventor 许国旺李佳赵欣捷陈世礼曾仲大路鑫
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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