Three-dimensional sphere-gathering culture chamber mold and method of screening concentrations of chemotherapeutic drugs in cancer cells

Abstract

The invention provides a three-dimensional sphere-gathering culture chamber mold and a method of screening concentrations of chemotherapeutic drugs in cancer cells, wherein the three-dimensional sphere-gathering culture chamber mold comprises at least one mold post for making cell culture holes via casting; each mold post a first segment, a second segment and a semisphere connected coaxially in sequence in a casting insertion direction, the axial length of the first segment is 20-30 times greater than the radius of the semisphere, the axial length of the second segment is 10-15 times greater than the radius of the semisphere, the second segment includes at least two buffer segments stacked, the sidewall of each buffer segment is inclined to the axis of the mold post in the insertion direction, and the inclination angles are different. The three-dimensional sphere-gathering culture chamber mold provided herein is suitable for making cell culture holes to simulate the human environment on an existing orifice plate through casting, so that efficient three-dimensional cell culture is achieved. In addition, the buffer segments enable the cell culture holes to correspondingly form a buffer chamber with multilayer buffer structure, so that cell damage caused during liquid changing is avoided.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a three-dimensional sphere culture cavity mold and a screening method for cancer cell chemotherapy drug concentration using the three-dimensional sphere culture cavity mold. Background technique [0002] Liver cancer is one of the most common malignant tumors in the world, so the treatment of liver cancer has very important significance and influence. Most patients with liver cancer have liver dysfunction and low tolerance to systemic chemotherapy, and liver cancer cells are resistant to most traditional chemotherapy drugs. Therefore, selecting the most sensitive anticancer drug for each liver cancer patient can effectively reduce the patient's exposure to chemotherapy drugs and help improve the treatment effect of the disease. [0003] In the process of culturing cancer cells, the traditional two-dimensional cell culture method is that the cells grow on a single layer of glass or pl...

AI Technical Summary

Problems solved by technology

[0003] In the process of cultivating cancer cells, the traditional two-dimensional cell culture method is that cells grow on a single layer of glass or plastic plane, which has good stretchability, but it is difficult to simulate the real situation in vivo

At the same time, due to the structure of the culture vessel, it is easily affected by fluid disturbance during the culture process, resulting in damage or even death of the cultured cells

However, the general three-dimensional cell culture method needs to specially manufacture cell culture wells that simulate the in vivo environment, which is expensive and cannot be reused.

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