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Purpose of hypericin to preparation of relevant products capable of restraining activity of glucosidase

A technology of glucosidase and hypericin, which is applied in the application field of hypericin, and achieves the effect of excellent inhibitory ability

Inactive Publication Date: 2019-07-19
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the inhibitory effect of hypericin on α-glucosidase

Method used

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  • Purpose of hypericin to preparation of relevant products capable of restraining activity of glucosidase
  • Purpose of hypericin to preparation of relevant products capable of restraining activity of glucosidase
  • Purpose of hypericin to preparation of relevant products capable of restraining activity of glucosidase

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Instruments and reagents

[0031] NaH 2 PO 4 2H 2 O (Tianjin Damao Chemical Reagent Factory);

[0032] Na 2 HPO 4 12H 2 O (Tianjin Damao Chemical Reagent Factory);

[0033] Anhydrous Na 2 CO 3 (Hongyan Reagent Factory, Hedong District, Tianjin);

[0034] Yeast α-glucosidase (US sigma company, 100UN);

[0035] pNPG (Aladdin Reagent (Shanghai) Co., Ltd.);

[0036] Acarbose (Shanghai Yuanye Technology Co., Ltd.);

[0037] Hypericin (Shanghai Yuanye Technology Co., Ltd.);

[0038] Microplate reader (BioTek company, model: EPOCH2);

[0039] Constant temperature oscillator (THOMMO SHAKER, model: BE-9010).

[0040] 2. Experimental method

[0041] 1. Preparation of reagents

[0042] (1) Configuration of liquid A: NaH 2 PO 4 2H 2 O Weigh 15.603g, dilute to 500mL, store at 4°C in a brown bottle for later use.

[0043] (2) Configuration of liquid B: Na 2 HPO 4 12H 2 O Weigh 35.822g, dilute to 500mL, store at 4°C in a brown bottle for later use.

[0044] ...

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Abstract

The invention discloses the purpose of hypericin to preparation of relevant products capable of restraining activity of glucosidase. The experiment proves that the hypericin can effectively restrain the activity of alpha-glucosidase, the 50% inhibiting concentration IC 50 value of the hypericin on the alpha-glucosidase is only 0.00498mg / mL, the restraining capacity of the hypericin is better thanthat of an existing alpha-glucosidase inhibitor, and the hypericin can be used for preparing products capable of regulating glucose metabolism disorders, reducing postprandial blood sugar, preventingand / or treating impaired glucose tolerance and / or diabetes and complications thereof, can also be used for preparing medicines capable of preventing and / or treating diseases using alpha-glucosidases as a target point, including obesity, hypertension, chronic hepatitis B, AIDS, tumor and the like, can even be used for preparing the alpha-glucosidase inhibitor, and is safe and efficient.

Description

technical field [0001] The invention relates to the application field of hypericin, in particular to the application of hypericin in the preparation of related products inhibiting glucosidase activity. Background technique [0002] α-glucosidase plays an important role in the process of food absorption and must be combined with it before food can be digested and absorbed. The hypoglycemic mechanism of α-glucosidase inhibitors is to slow down the decomposition of starch into glucose by inhibiting α-glucosidase on the intestinal mucosa, reducing and delaying the absorption of glucose by the small intestine, so as to lower blood sugar and have a positive effect on postprandial The effect of hyperglycemia is more obvious. Glucosidase inhibitors do not stimulate insulin secretion, and they usually do not cause hypoglycemia when used alone, so they can help reduce blood sugar fluctuations. It can significantly reduce the probability of cardiovascular disease in diabetic patients...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P3/10A61P3/04A61P9/12A61P1/16A61P31/20A61P31/18A61P35/00A23L33/105A61K36/38
CPCA23V2002/00A61K31/122A61K36/38A23L33/105A61P1/16A61P3/04A61P3/10A61P9/12A61P31/18A61P31/20A61P35/00A23V2200/308A23V2200/328A23V2200/332A23V2200/326A23V2200/30
Inventor 董琦王洪伦胡娜
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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