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MBR membrane pollution control method based on microbial quorum sensing quenching

A quorum sensing and control method technology, applied in the field of MBR membrane fouling control based on microbial quorum sensing quenching, can solve the problems of membrane fiber clogging, limiting QQ performance membrane fouling control, etc. simple effect

Inactive Publication Date: 2019-07-19
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to avoid clogging caused by sludge particles, excessive use of QQ immobilized products, membrane fibers, this fact limits the performance of QQ and the control of membrane fouling

Method used

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  • MBR membrane pollution control method based on microbial quorum sensing quenching
  • MBR membrane pollution control method based on microbial quorum sensing quenching
  • MBR membrane pollution control method based on microbial quorum sensing quenching

Examples

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Embodiment 1

[0039] A kind of MBR membrane fouling control method based on microbial quorum sensing quenching as an embodiment of the present invention, described method comprises the following steps:

[0040] (1) Centrifuge the quorum sensing quenching bacteria after culturing them in a medium at 30°C for 12 hours to obtain the quorum sensing quenching bacterial cells, and disperse the quorum sensing quenching bacterial cells into a solution of polyvinyl alcohol and sodium alginate , and drop calcium chloride and boric acid mixed solution as a polymer curing agent to form microbead-like immobilized quorum sensing quenching bacteria, and microbead-like immobilized quorum sensing quenching bacteria were carried out in sodium sulfate solution further cross-linking;

[0041] (2) dropping the immobilized quorum sensing quenching bacteria into the pretreatment tank before the MBR reaction tank, and retaining the immobilized quorum sensing quenching bacteria in the pretreatment tank;

[0042] T...

Embodiment 2

[0046] A kind of MBR membrane fouling control method based on microbial quorum sensing quenching as an embodiment of the present invention, described method comprises the following steps:

[0047] (1) Centrifuge the quorum sensing quenching bacteria after culturing them in a medium at 30°C for 12 hours to obtain the quorum sensing quenching bacterial cells, and disperse the quorum sensing quenching bacterial cells into a solution of polyvinyl alcohol and sodium alginate , and drop calcium chloride and boric acid mixed solution as a polymer curing agent to form microbead-like immobilized quorum sensing quenching bacteria, and microbead-like immobilized quorum sensing quenching bacteria were carried out in sodium sulfate solution further cross-linking;

[0048] (2) dropping the immobilized quorum sensing quenching bacteria into the pretreatment tank before the MBR reaction tank, and retaining the immobilized quorum sensing quenching bacteria in the pretreatment tank;

[0049] (...

Embodiment 3

[0056] As a MBR membrane fouling control method based on microbial quorum sensing quenching according to an embodiment of the present invention, the only difference between this embodiment and Example 2 is that microbead-shaped immobilized quorum sensing is added to the MBR reaction pool. The density of quenched bacteria was 1% v / v.

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Abstract

The invention provides an MBR (Membrane Bioreactor) membrane pollution control method based on microbial quorum sensing quenching. The method comprises the following steps: (1) immobilizing quorum sensing quenching bacterium thalli by using an immobilized high polymer material to obtain immobilized quorum sensing quenching bacteria; and (2) putting the immobilized quorum sensing quenching bacteriainto a pretreatment tank in front of an MBR reaction tank, and intercepting the immobilized quorum sensing quenching bacteria in the pretreatment tank, wherein the quorum sensing quenching bacteria can degrade acyl homoserine lactone. According to the invention, the input of signal molecules entering the MBR reaction tank can be effectively reduced, the control effect on MBR membrane pollution isobvious, and the period of MBR membrane pollution is increased by 100-200%. The defect that the number of immobilized quorum sensing quenching bacteria needs to be strictly controlled in in-situ QQ is overcome, and the performance of the quorum sensing quenching bacteria and control over membrane pollution can be fully exerted. The method is simple in operation process and convenient for application of a sewage treatment plant.

Description

technical field [0001] The invention relates to the technical field of sewage biological treatment, in particular to an MBR membrane pollution control method based on microbial quorum sensing quenching. Background technique [0002] Microbes are not a group of solitary organisms, they also have the ability to socialize. Bacteria can synthesize, release and detect signal molecules in aquatic environment, so they can exchange information, which is called quorum sensing (QS). QS can regulate a series of biological behaviors, such as biofilm formation. Recently, it was reported that membrane fouling in membrane bioreactors (MBRs) could be attributed to QS, leading to the formation of a series of strategies to suppress QS to alleviate membrane fouling in MBRs, called quorum sensing quenching (QQ). [0003] The method of QQ used in MBR is mainly to add QQ enzyme or QQ bacteria (combined with bio-encapsulation method) in MBR to degrade the signal molecules in the bioreactor. App...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/12
CPCC02F3/1268C02F2303/14Y02W10/10
Inventor 余华荣杜晨宇瞿芳术赫俊国荣宏伟方茜
Owner GUANGZHOU UNIVERSITY
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