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Freeze-dried powder containing sheep mesenchymal stem cell exosome and preparation method thereof

A technology of stem cells and exosomes, applied in the field of medical cosmetology, can solve problems such as unclear ingredients and mechanism of action, and achieve the effects of protecting biological activity, delaying aging, and accelerating skin repair.

Active Publication Date: 2019-07-23
广州优理氏生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are many types of exosomes, and the c

Method used

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  • Freeze-dried powder containing sheep mesenchymal stem cell exosome and preparation method thereof
  • Freeze-dried powder containing sheep mesenchymal stem cell exosome and preparation method thereof
  • Freeze-dried powder containing sheep mesenchymal stem cell exosome and preparation method thereof

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Effect test

preparation example Construction

[0031] figure 1 It is the sheep umbilical cord (A), Wharton's jelly (B), and mesenchymal stem cells crawled out of Wharton's jelly from the sheep umbilical cord (A) and Wharton's jelly (B) collected in the present invention containing sheep mesenchymal stem cell exosome freeze-dried powder and its preparation method. (C) The schematic diagram, such as figure 1 As shown, mesenchymal stem cells are extracted from sheep umbilical cord, and the cell culture method is tissue-adhered culture. The operation steps are as follows:

[0032] 1) Aseptically collect sheep umbilical cords that pass the inspection and quarantine;

[0033] 2) Clean the residual blood on the sheep umbilical cord, carefully remove two arteries and one vein in the sheep umbilical cord, separate the Wharton's jelly, and cut the Wharton's jelly into small pieces with a size of about 2 mm;

[0034] 3) Inoculate a small piece of Wharton's jelly in the serum-free sheep umbilical cord mesenchymal stem cell culture m...

Embodiment 1

[0044] The composition and proportion of the freeze-dried powder are: 0.01% stem cell exosomes, 6% mannan, 0.5% sodium hyaluronate, 0.1% ginseng extract, 0.1% ceramide, 0.1% nicotinamide, and the rest are free bacteria water.

Embodiment 2

[0046] The composition and proportion of the freeze-dried powder are: 0.05% stem cell exosomes, 8% mannan, 1% sodium hyaluronate, 1% ginseng extract, 1% ceramide, 1% nicotinamide, and the rest are free bacteria water.

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Abstract

The invention discloses a freeze-dried powder containing sheep mesenchymal stem cell exosome. The freeze-dried powder comprises the following components: stem cell exosome, mannan, sodium hyaluronate,ginseng extract, ceramide, nicotinamide and sterile water. According to the invention, the freeze-dried powder containing sheep mesenchymal stem cell exosome and the preparation method thereof are adopted, and the freeze-dried powder has the effects of accelerating skin repair, desalting fine lines and delaying aging.

Description

technical field [0001] The invention relates to the technical field of medical cosmetology, in particular to a freeze-dried powder containing sheep mesenchymal stem cell exosomes and a preparation method thereof. Background technique [0002] The skin covers the surface of the body and is rich in tissues such as blood vessels, nerves, and sweat glands. As the first natural barrier, the skin is in direct contact with the outside world and can prevent external stimuli such as microbial invasion and UV damage. With the excessive use of cosmetics, many people have symptoms such as thinning of the cuticle of the facial skin, easy allergies, redness, and easy damage. However, there is currently no effective repair product on the market. [0003] Stem cell therapy has shown great potential in the field of regenerative medicine, especially umbilical cord mesenchymal stem cells have attracted more and more attention due to their advantages such as wide source, convenient extraction,...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/98A61Q19/00A61Q19/08
CPCA61K8/982A61K8/99A61Q19/00A61Q19/08
Inventor 李占鸿
Owner 广州优理氏生物科技有限公司
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