Endothelial cell markers and kit for predicting cancer immunotherapy effect

A technology for endothelial cells and therapeutic effects, applied to the field of endothelial cell markers and kits, can solve the problems of low accuracy and no response to immune checkpoint inhibitors, achieve high accuracy, and is suitable for popularization, promotion, and operation. simple effect

Active Publication Date: 2019-07-26
NANTONG UNIVERSITY
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Problems solved by technology

However, the method based on the level of PD-L1 expression is still controversial (reference: Shen, X., & Zhao, B. (2018). Efficacy of PD-1 or PD-L1 inhibitors and PD-L1 expression status in ca

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  • Endothelial cell markers and kit for predicting cancer immunotherapy effect
  • Endothelial cell markers and kit for predicting cancer immunotherapy effect
  • Endothelial cell markers and kit for predicting cancer immunotherapy effect

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Embodiment Construction

[0026] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will describe in detail with reference to the drawings and specific embodiments.

[0027] The tumor microenvironment plays a key role in cancer therapy. Differences in the tumor microenvironment largely determine the response of tumor cells to therapy. Endothelial cells are an important determinant of tumor internal environment, so we systematically studied the proportion and status of endothelial cells in various tumors.

[0028] Based on the above research, the present invention provides an endothelial cell marker for predicting the effect of cancer immunotherapy, the endothelial cell marker is VWF, HYAC2, FAM124B, ROBO4, ARHGEF15, ACVRL1, TIE1, MMRN2.

[0029] Specifically, the present invention designs a KIT, which includes the use of RT-PCR to detect the expression of endothelial cell marker molecules in the tumor, VWF, HYAC2, FA...

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Abstract

The invention provides endothelial cell markers and kit for predicting a cancer immunotherapy effect. The endothelial cell markers are VWF, HYAC2, FAM124B, ROBO4, ARHGEF15, ACVRL1, TIE1 and MMRN2. Thekit comprises at least specific primers for VWF, HYAC2, FAM124B, ROBO4, ARHGEF15, ACVRL1, TIE1 or MMRN2 genes. The kit includes at least a specific primer for the VWF, HYAC2, FAM124B, ROBO4, ARHGEF15, ACVRL1, TIE1 or MMRN2 genes. In the technical scheme of the invention, compared with a traditional PD-L1 expression amount based biomarker for predicting a cancer immune checkpoint blocking treatment effect is higher in accuracy, and the curative effect of a cancer patient using the immune checkpoint blocking treatment can be better predicted. In addition, only a basic RT-qPCR technology is needed for detection, the operation is relatively simple, the cost is low, the endothelial cell markers and the kit can be operated by a medical institution with a clinical laboratory, are suitable for popularization and popularization, need fewer tissue samples and are suitable for early tumor application.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an endothelial cell marker and a kit for predicting the effect of cancer immunotherapy. Background technique [0002] Currently, tumor immunotherapy based on the PD-1 / PD-L1 immune checkpoint is hot and climaxing. Immune checkpoint blockade therapy (immune checkpoint inhibitor) is a treatment method that uses the immune system to kill tumors by regulating the activity of T cells in patients. Immune checkpoints can inhibit the activity of T cells, and may be used by tumors in tumor tissues to form immune escape. Common immune checkpoints include CTLA4, PD-1, LAG-3, TIM-3, etc., by blocking immune checkpoints Can release suppressed T cell activity. The principle of this therapy is: by blocking the PD-1 / PD-L1 immune checkpoint pathway, the tumor can no longer escape the immune system, thereby promoting the body's own immune system to clear tumor cells. This therapy has been approved b...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158
Inventor 吴英成毛仁芳范义辉
Owner NANTONG UNIVERSITY
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