Micro-immunofluorescence detection method without cell loss

Abstract

The invention discloses a micro-immunofluorescence detection method without cell loss. The micro-immunofluorescence detection method comprises the steps of: performing 0.22 um Spin-X Centrifuge Tube sterilization; transferring cells into a sterilized 0.22 um Spin-X Centrifuge Tube for carrying out subsequent steps directly or carrying out subsequent steps fter cultivation; and performing the subsequent steps of PBS buffer solution rinsing, 4% paraformaldehyde fixation, methanol treatment on ice, 0.3 M glycine confining liquid treatment, 4% BSA confining liquid treatment, fluorescence primary antibody room temperature dark incubation, staining in DAPI staining solution, slide sealing after placing a filtering membrane in a slide, and observation of the slide under a laser scanning cofocal microscope. The method makes up for the shortcoming that the immunofluorescence technique is difficult to achieve precise screening of specific cells, and provides a simple, precise and visual technique for screening specific cells in a mixed cell population.

Description

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AI Technical Summary

Benefits of technology

This patented method uses specialized reagents called primers specifically target cancerous tissue cells from healthy individuals' circulating lymphatic system. These primer molecules are attached to various substances like albumen, collagen, gelatin, etc., making them easy targets for imagining these areas during histopathology analysis. By selecting appropriate ones for each patient by measuring their own unique gene expression levels, this technique allows researches looking at how well they work out over time without sacrificing accuracy. Overall, this process provides technical means for identifying and analyzing rare diseases such as autoimmune disorders through flow cytometry techniques.

Problems solved by technology

Technologies have developed overall, these technics aim to provide better ways to detect diseases like cancer through various means including immune fibroupletance assay(IFA). These include immunofungluximab (FIS)-based tests, flowcytelectron emission tomography (FECT), quantum dots, and laser scanning imagers. While IFAs allow precise detection of disease states within living organisms, their limitations exist due to factors like weakness caused by natural fixations between different parts of organoids called chromatophoric receptors. Fluorescences offer improved accuracy compared to conventional FIBS while being more sensitive than optically based systems. Additionally, there may still remain issues associated with current immunosuppressive procedures involving enzyme activation and deposition.

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