A Penicillium oxalicum phospholyticum with broad-spectrum acid production
A technology of Penicillium oxalicum and strains, applied in the field of environmental biology, can solve limited problems, achieve the effect of improving soil fertility, good application value, and promoting comprehensive treatment of heavy metal soil pollution
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Embodiment 1
[0026] [Implementation 1] Screening of Phosphorus Solubilizing Fungi
[0027] The experimental soil samples were collected from the activated sludge of the sewage treatment plant in Yunxi District Industrial Park, Yueyang City, Hunan Province, and were air-dried for 48 hours immediately after collection. After removing excess impurities, they were ground through a 20-mesh soil sieve, and the excess soil samples were stored in a refrigerator at 4°C. . Take 10g of sieved soil sample, add 100mL sterilized liquid enrichment medium (containing sterile glass beads) (enrichment medium components: peptone 10g / L, NaCl 1.0g / L, KH 2 PO 3 1.0g / L, pH=7), shaking at 150rpm for 7 days. After culturing for 1 week, take 1 mL of the culture solution in the enriched medium, and take 1 mL of the supernatant and dilute it to 10 -5 , take 10 of them -4 ,10 -5 ,10 -6 200 μL of the solution under the three dilution gradients were placed in the phosphate-dissolving medium with calcium phosphat...
Embodiment 2
[0029] [Example 2] identification of bacterial strains
[0030] Inoculate the purified bacterial strain on solid PDA medium (ingredients: potato 200g / L, glucose 20g / L, agar 20g / L, pH=7) and cultivate for 5 days to observe the bacterial colony morphological characteristics of the bacterial strain on the solid plate, and then pick some Observe the microstructure of the bacterial colony under an optical microscope, and record the morphological characteristics of the bacterial strain: the colony is fluffy, the interbranches are bifurcated, mostly trident, and the center is asymmetrically bifurcated; the color of the spore is green, and the shape is oval , spherical, about 0.10 μm in diameter; spore peduncle short and thick, with obvious branches, fork-like acute-angled branches, asymmetrical branches, about 0.25 μm in length.
[0031] Fungal ITS rDNA sequencing included strain DNA extraction, ITS rDNA in vitro amplification (using ITS universal primers ITS1 and ITS4) and gene sequ...
Embodiment 3
[0032] [Example 3] Degradation characteristics analysis of bacterial strains to calcium phosphate and iron phosphate
[0033] Using liquid phosphate-dissolving medium, the final concentrations after adding calcium phosphate and iron phosphate were set to 5g / L and 2g / L respectively. Before the measurement, inoculate the strain in the phosphorus-dissolving slant medium and cultivate it, activate it at 28°C for 72 hours, then take 1mL of sterile water, add it to the slant, and scrape the spores and hyphae of the slant fungus into the sterile water to form a spore suspension solution was added to the culture medium at a volume ratio of 5%. All the cultures were shaken at 28° C. for 14 days based on a shaker at 150 rpm, and the pH value and soluble phosphorus content of the medium were measured respectively. Finally, PSF-4 can dissolve 2071mg / L of calcium phosphate with a phosphorus dissolution rate of 100% for 5g / L calcium phosphate, and 198mg / L for 2g / L iron phosphate with a pho...
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