Preparation method of luminescent collagen polypeptide-lanthanide-series metal composite nanomaterial with cell adhesion function

A composite nanomaterial and collagen polypeptide technology is applied in the field of preparation of luminescent collagen polypeptide-lanthanide metal composite nanomaterials, which can solve problems such as the lack of self-assembled polypeptides, achieve good cell adhesion performance, and the preparation process is environmentally friendly and stable. good effect

Active Publication Date: 2019-08-16
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current research is limited to the microstructure of biomimetic collagen, and self-assembled polypeptides that can biomimetic collagen functions are still very scarce.

Method used

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  • Preparation method of luminescent collagen polypeptide-lanthanide-series metal composite nanomaterial with cell adhesion function
  • Preparation method of luminescent collagen polypeptide-lanthanide-series metal composite nanomaterial with cell adhesion function
  • Preparation method of luminescent collagen polypeptide-lanthanide-series metal composite nanomaterial with cell adhesion function

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preparation example Construction

[0034] The present invention will be described in detail below in conjunction with the accompanying drawings. In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments. Such as Figure 1-Figure 5 As shown, a method for preparing a luminescent collagen polypeptide-lanthanide metal composite nanomaterial with cell adhesion function comprises the following steps:

[0035] (1) Design of collagen peptides

[0036] Collagen polypeptides with amino acid sequences are designed, including: designing (Gly-Pro-Hyp)n or (Gly-Pro-Pro)n sequences rich in (Gly-Pro-Pro)n, so that the polypeptides form a triple helical structure; Sequence; the amino acids at both ends of the polypeptide and 1-3 positions in the middle of the polypeptide are designed to be aspartic acid or glutamic acid, so that the aspartic acid or glutamic ...

Embodiment 1

[0049] (1) Design of collagen peptides

[0050] The designed collagen peptide sequence is DD(GPO) 3 GDOGPOGFOGERGPOGDO (GPO) 3 DD.

[0051] (2) Synthesis of Collagen Peptides

[0052] The designed collagen peptides are synthesized by Fmoc solid-phase synthesis method and step-by-step synthesis with a manual synthesizer, including: a. Resin treatment swelling; b. Activation of amino acids; c. Condensation reaction; d. α-Amino deprotection; e. Extended peptide resin; f, cut peptide resin; g, separation and purification of peptide.

[0053] (3) Preparation of collagen polypeptide-lanthanide metal composite nanomaterials

[0054] Prepare a collagen polypeptide solution with a concentration of 50mg / mL; prepare nitrate solutions of different lanthanide metals with a concentration of 50mg / mL; then mix; place the above mixed solution in a thermostat at 25°C for 24hrs; put the reaction product at 8000rpm Solid-liquid separation was carried out to obtain solid precipitation.

[00...

Embodiment 2

[0063] (1) Design of collagen peptides

[0064] The designed collagen peptide sequence is EE(GPO) 3 GEOGPOGFOGERGPOGEO (GPO) 3 E.E.

[0065] (2) Synthesis of Collagen Peptides

[0066] The designed collagen peptides are synthesized by Fmoc solid-phase synthesis method and step-by-step synthesis with a manual synthesizer, including: a. Resin treatment swelling; b. Activation of amino acids; c. Condensation reaction; d. α-Amino deprotection; e. Extended peptide resin; f, cut peptide resin; g, separation and purification of peptide.

[0067] (3) Preparation of collagen polypeptide-lanthanide metal composite nanomaterials

[0068] Prepare a collagen polypeptide solution with a concentration of 100mg / mL; prepare nitrate solutions of different lanthanide metals with a concentration of 100mg / mL; then mix; place the above mixed solution in a thermostat at 25°C for 36hrs; put the reaction product at 8000rpm Solid-liquid separation was carried out to obtain solid precipitation.

...

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Abstract

The invention discloses a preparation method of a luminescent collagen polypeptide-lanthanide-series metal composite nanomaterial with cell adhesion function. The preparation method includes the steps: (1) design of collagen polypeptide; (2) synthesis of collagen polypeptide; (3) preparation of a collagen polypeptide-lanthanide-series metal composite nanomaterial; (4) luminescent properties of thecomposite nanomaterial; and (5) cell adhesion function of the composite nanomaterial. The used collagen polypeptide can self-assemble to form a nanofiber material with similar microstructure to natural collagen under the mediation of lanthanide-series metal ions under a condition of wide range of pH. The composite fiber material not only has excellent luminescent properties, but also exhibits good cell adhesion function, provides a new strategy for the structure and function of biomimetic natural collagen and has great development potential in tissue engineering and regenerative medicine.

Description

technical field [0001] The invention relates to a preparation method of a luminous collagen polypeptide-lanthanide metal composite nanomaterial with cell adhesion function, and belongs to the technical field of biological nanomaterial preparation. Background technique [0002] Nanotechnology refers to the technology of studying the characteristics of the laws of motion of electrons, atoms and molecules and the processing of substances and materials on the scale of 0.1-100 nanometers. [0003] Nanomaterials have a close relationship with organisms in size. For example, the ribonucleic acid protein complex that constitutes one of the elements of life has a linear dimension between 15-20nm, and the size of various viruses in organisms is also in the nanoscale range. Nano biomedical materials are the intersection of nanomaterials and biomedical materials, and various composite materials are made by combining nanoparticles with other materials. With the further deepening of res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00B82Y40/00
CPCB82Y40/00C12N5/0068C12N2533/20C12N2533/54
Inventor 肖建喜孙秀霞
Owner LANZHOU UNIVERSITY
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