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Method for determining mangiferin in common bombax flower and quality evaluation method of common bombax flower

A method of determination, the technology of mangiferin, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of convenient detection and improved extraction rate

Inactive Publication Date: 2019-08-20
青海省药品检验检测院
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for determining mangiferin in common bombax flower and quality evaluation method of common bombax flower
  • Method for determining mangiferin in common bombax flower and quality evaluation method of common bombax flower
  • Method for determining mangiferin in common bombax flower and quality evaluation method of common bombax flower

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Embodiment

[0028] 1. Determination of mangiferin as the target component

[0029] Respectively with different mobile phase systems (acetonitrile-0.1% phosphoric acid solution, methanol-0.1% phosphoric acid solution) gradient elution, different solvent extraction samples, investigate the chromatogram information under 258nm, 270nm, 310nm and 360nm respectively, the result sample is in the above In the fingerprints formed under various chromatographic conditions, the chromatographic peaks of mangiferin are the highest peaks, indicating that in the methods investigated, the content of mangiferin in kapok is the highest, thus confirming that kapok is the determination component. The chromatogram information is the most abundant at 258nm, choose 258nm to establish a fingerprint, such as figure 1 shown. The chromatographic peak at 17.585min in the fingerprint is the mangiferin peak, and the integrated value of the peak area is the largest.

[0030] 2. Chromatographic conditions

[0031] Chr...

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Abstract

The invention discloses a method for determining mangiferin in common bombax flower and a quality evaluation method of common bombax flower, and belongs to the field of drug detection. The method fordetermining mangiferin in common bombax flower comprises the following steps that a proper amount of pure mangiferin is taken and added with 60% methanol to prepare a contrast solution, and 1ml of thecontrast solution includes 20 microgram of mangiferin; 0.5g of common bombax flower sample powder is taken and added with 25ml of 60% methanol, the weight is measured, ultrasonic processing is carried out for 1 hour, cooling is carried out, the weight is measured again, 60% of methanol is used to supplement the reduced weight, a mixture is shaken up and filtered, a subsequent filtrate is taken, and a solution to be detected is obtained; and 10 microliter of the contrast solution and 10 microliter of the solution to be detected are sucked and injected to a liquid chromatograph, and the contentof mangiferin is obtained via a content determination method. The chromatographic peak of the contrast solution is compared with that of the solution to be detected for calculation to obtain the content of mangiferin in common bombax flower conveniently. The quality of the common bombax flower can be also evaluated effectively.

Description

technical field [0001] The invention relates to the field of drug detection, in particular to a method for measuring mangiferin in kapok and a method for evaluating its quality. Background technique [0002] Kapok is a large deciduous tree, up to 25 meters high, with off-white bark. The trunk of young trees usually has conical thick thorns; the branches are flat. Kapok has high medicinal value and is commonly used in traditional Chinese medicine and Tibetan medicine. The current standard "Chinese Pharmacopoeia" 2015 edition only contains the characteristics, microscopic, TLC identification methods and leaching items of kapok, among which the reference material of TLC identification method is kapok reference medicinal material, and kapok is not included. Qualitative or quantitative determination of chemical constituents in cotton. Only the character of kapok (Tibetan name: Naga Gesa) is recorded in the first volume of Tibetan medicine in the "Drug Standards of the Ministry ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047
Inventor 宋霞吴立斌刘亚蓉乔亚玲张春平
Owner 青海省药品检验检测院
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