Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of shuteria root total flavonoid extract

A technology of ephedra and total flavonoids is applied in the field of medical technology and health care products, which can solve problems such as no reports on research, and achieve the effects of inhibiting the generation of various inflammatory factors and improving pathological changes.

Pending Publication Date: 2019-08-23
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the help of modern scientific and technological means, finding the effective parts and active ingredients of antagonizing asthma from ethnic medicines is a new approach to asthma treatment and the main development direction of new drug research and development. However, the research on the use of total flavonoids extract of copper money ephedra to prevent and treat bronchial asthma has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of shuteria root total flavonoid extract
  • Application of shuteria root total flavonoid extract
  • Application of shuteria root total flavonoid extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Preparation of total flavonoids extract

[0052] 1. Take 102g of copper coin ephedra and grind it to a particle size of 40 mesh;

[0053] 2. Extract 1 L of 70% (v / v) aqueous ethanol solution under reflux for 3 times at a temperature of 65°C for 2 hours each time, combine the extracts three times, and recover the extraction solvent to obtain 13.3 g of the total extract;

[0054] 3. Disperse the total extract with 500ml of water, centrifuge at 20,000 rpm in a tube centrifuge to obtain 420ml of centrifugate, degrease 2 times with 1.2L of petroleum ether, and extract 3 times with 1L of ethyl acetate. Merge the ethyl acetate layers, reclaim the extractant to obtain ethyl acetate extract 8.02g;

[0055] 4. Dilute the obtained ethyl acetate extract with 150ml of water to a concentration of 5% by mass. Take 400g of polyanionic cellulose and pack it into a column under pressure, equilibrate the column with methanol aqueous solution with a concentration of 40% by vol...

Embodiment 2

[0057] Example 2: Therapeutic effect of copper money ephedra total flavonoids extract on asthma in OVA-sensitized mice

[0058] 1. Reagents: OVA (batch number: 9006-59-1) and dexamethasone (batch number: BCBV3214) were purchased from Sigma; EotaxinELISA detection kit (batch number: 2130802231), IL-4 ELISA detection kit (batch number: A20480141) , IL-5ELISA Detection Kit (Lot No.: A20580821), IL-13 ELISA Detection Kit (Lot No.: A21381132), IL-25ELISA Detection Kit (Lot No.: M181225-007a), IL-33 ELISA Detection Kit (Lot No.: A23380314), INF-γ ELISA kit (lot number: A201B81053), MUC5AC ELISA kit (lot number: ZKVBMELGV3), TSLP ELISA kit (lot number: A26580933), IL-10 ELISA kit (lot number: A21090121) , Total IgE ELISA detection kit (batch number: A27581115) was purchased from Hangzhou Lianke Biotechnology Co., Ltd.; OVA-IgE detection kit (batch number: 0544983) was purchased from Aimeijie Technology Co., Ltd.

[0059] , Instruments: ultrasonic nebulizer (INQUA NEB plus, Germany B...

Embodiment 3

[0076] Example 3 Therapeutic effect of the total flavonoids extract of Ephedra chinensis on asthma in obese mice

[0077] 1. Reagents: house dust mite (batch number: 331422) was purchased from GREER Laboratory in the United States; high-fat feed (29% carbohydrate, 55% fat, 16% protein, batch number: MD12032) was purchased from Jiangsu Medison Biomedical Co., Ltd.; Dexamethasone (batch number: BCBV3214) was purchased from Sigma; IL-1β ELISA detection kit (batch number: A201B81053), IL-17A ELISA detection kit (batch number: A21780741), IL-22 ELISA detection kit (batch number: A22280933 ), MUC5AC ELISA detection kit (batch number: ZKVBMELGV3) was purchased from Hangzhou Lianke Biotechnology Co., Ltd.; IL-8 ELISA detection kit (batch number: M181211-104a) was purchased from Xinbosheng Biotechnology Co., Ltd.

[0078] , Instruments: ultrasonic nebulizer (INQUA NEB plus, Germany Bairui company); multifunctional microplate reader (Spectramax plus 384, PEI-Genesis); desktop high-speed...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a shuteria root total flavonoid extract in preparation of medicines for preventing or treating bronchial asthma. Pharmacological experiment research shows that the shuteria root total flavonoid extract can obviously improve the pathological changes of lung tissue of asthma mice and inhibit generation of multiple inflammatory factors in serum and alveolar lavage fluid. The shuteria root total flavonoid extract is capable of effectively antagonizing asthma, is suitable for preparing new medicines for preventing and treating bronchial asthma, and has wide application prospects and huge potential value.

Description

technical field [0001] The invention belongs to the field of medical technology and health care products, and in particular relates to the application of total flavonoids extract of ephedra ephedrae in the prevention and treatment of bronchial asthma. Background technique [0002] Bronchial asthma (asthma for short, Asthma) is a heterogeneous disease characterized by chronic airway inflammation, airway hyperresponsiveness, and airway mucus hypersecretion. The main clinical symptoms are recurrent wheezing, shortness of breath, chest tightness, coughing, etc. , multiple at night and early morning. The disease is caused by the combination of genetic factors and environmental factors, and is a common and frequently-occurring disease worldwide. According to statistics, there are currently at least 300 million patients in the world, about 30 million in China, and the number of patients in Western Europe has doubled in the past 10 years. With economic development and changes in pe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/17A61P11/06
CPCA61K36/17A61P11/06
Inventor 杨竹雅黄丰刘录马晓霞谭文红
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com