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A kind of magnetic nano particle, its preparation method and its application in transfection reagent

A magnetic nanoparticle and nanoparticle technology, applied in the field of magnetic materials and nanotechnology research, can solve the problems of non-specific interaction of cytotoxic blood components, complicated operation, etc., and achieve low toxicity, simple operation, and enhanced transfection efficiency. Effect

Active Publication Date: 2021-07-27
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

[0005] In view of the defects of the prior art, the purpose of the present invention is to solve the complex operation of the magnetic nanoparticle and nucleic acid binding method used in the existing magnetic transfection technology, and the modification of the magnetic nanoparticle with a cationic polymer leads to cytotoxicity and non-specific interaction of blood components. Technical issues limiting clinical application

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  • A kind of magnetic nano particle, its preparation method and its application in transfection reagent

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Embodiment Construction

[0023] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not constitute a conflict with each other.

[0024] According to the clinical requirement of magnetic transfection, the present invention designs and prepares a magnetic nanoparticle with simple process, extremely low toxicity and positive charge on the surface as the carrier of magnetic transfection. The magnetic nanoparticles were synthesized in three steps. The first step is to synthesize ferric oxide nanoparticles; the second step is ...

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Abstract

The invention discloses a magnetic nanoparticle, a preparation method thereof and an application in a transfection reagent, comprising: synthesizing the magnetic nanoparticle by a co-precipitation method; adding the magnetic nanoparticle into a hydroxide ion solution to obtain a hydroxide ion Ion-modified iron ferric oxide nanoparticles; adding the hydroxide ion-modified magnetic nanoparticles into a metal cation solution to obtain positively charged magnetic nanoparticles on the surface for magnetic transfection. The magnetic nanoparticles synthesized by this method are small in particle size, suitable for endocytosis, and have positive charges distributed on the surface, which can bind to nucleic acid through electrostatic interaction, and play the role of concentrating and folding nucleic acid. When the magnetic nanoparticles provided by the invention are used for magnetic transfection, the safety risk of transfection to cells is effectively reduced, and the transfection efficiency is further enhanced.

Description

technical field [0001] The invention relates to the field of magnetic material and nanotechnology research, more specifically, to a magnetic nanoparticle, its preparation method and its application in transfection reagents. Background technique [0002] Gene transfection is the process of transferring or transporting nucleic acids with biological functions into cells and maintaining their biological functions. Gene transfection technology has been widely used in genome function research and gene therapy research. The former mainly includes research on gene expression regulation, signal transduction and drug screening, while the latter mainly focuses on gene therapy for tumors, AIDS and genetic diseases. Due to the large size and negative charge of nucleic acids, it is difficult to cross the negatively charged cell membrane through osmosis alone. Even if they enter the cell membrane through endocytosis, nucleic acids are likely to be degraded by lysosomes. In addition, ther...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/87
CPCC12N15/87
Inventor 曹全梁陈竞舸韩小涛
Owner HUAZHONG UNIV OF SCI & TECH
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