Method for measuring urea content in musk blood-activating stasis-removing paste

A technology for promoting blood circulation and removing blood stasis and removing blood stasis, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome methods, difficult urea content, and many interfering components, and achieves uniform material and liquid composition, good peak properties, Yield-enhancing effect

Inactive Publication Date: 2019-08-30
GUIZHOU LIANSHENG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for determining the content of urea in musk activating blood circulation and removing blood stasis ointment. The method for determining the urea content is relatively cumbersome, and because there are many interfering components in musk activating blood and removing blood stasis ointment, it is difficult to detect musk simply and accurately. The problem of urea content in Huoxuehuayu ointment

Method used

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  • Method for measuring urea content in musk blood-activating stasis-removing paste
  • Method for measuring urea content in musk blood-activating stasis-removing paste
  • Method for measuring urea content in musk blood-activating stasis-removing paste

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Embodiment 1

[0033] Embodiment 1: a kind of assay method of urea content in musk promoting blood circulation and removing blood stasis ointment, comprises the following steps:

[0034] 1) Prepare a reference solution of urea with methanol solution as solvent;

[0035] 2) Take Musk Huoxue Huayu Ointment and cut it to get the sample to be tested;

[0036] 3) Dissolve and extract the sample to be tested with ethanol solvent to obtain the test solution;

[0037] 4) Determination of the content of urea in the reference solution and the test solution by high performance liquid chromatography;

[0038] The chromatographic detection conditions are: amino-bonded silica gel is used as a filler, the mobile phase is acetonitrile-water solution with a volume fraction ratio of 93:7; the detection wavelength is 200nm; the injection volume is 20uL.

[0039] The ointment for promoting blood circulation and removing stasis of musk comprises the following ingredients: musk, notoginseng, safflower, salvia m...

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Abstract

The invention discloses a method for measuring the content of urea in musk blood-activating stasis-removing paste, which is characterized by comprising the following steps: 1) Preparing a reference solution of urea by using a methanol solution as a solvent; 2) Taking musk blood-activating stasis-removing paste, and shearing the paste to obtain a sample to be tested; 3) Dissolving and extracting the sample to be detected by using an ethanol solvent to obtain a sample solution; and 4) Measuring the content of urea in the reference solution and the sample solution by high performance liquid chromatography. The chromatographic detection conditions are as follows: amino bonded silica gel is used as a filling agent, and a mobile phase is an acetonitrile-water solution with a volume fraction ratio of 92:8; the detection wavelength is 190 nm; the sample size is 10 uL. The technical characteristics adopted by the invention solve the problems that the existing method for measuring the content ofurea is complicated, and the content of urea in the musk blood-activating stasis-removing paste is difficult to simply, conveniently and accurately detect due to more interference components in the musk blood-activating stasis-removing paste.

Description

technical field [0001] The invention relates to the field of content detection of pharmaceutical ingredients, in particular to a method for determining the content of urea in musk promoting blood circulation and removing blood stasis ointment. Background technique [0002] Musk Huoxuehuayu ointment has the effects of promoting blood circulation and removing blood stasis, reducing inflammation and relieving pain; it is used for joint sprains, soft tissue contusions, acute lumbar sprains, lumbar muscle strain, periarthritis of the shoulder, non-ulcerated chilblains, and nodular redness; Musk Huoxuehuayu ointment is used as A kind of Chinese patent medicine, its ingredients include not only several traditional Chinese medicine preparations, but also western medicine ingredients; in order to test the active ingredients in Musk Huoxue Huayu Ointment, there have been methods for determining the content of various ingredients in it, but few Among them, the determination method of u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34
CPCG01N30/02G01N30/06G01N30/34
Inventor 罗玉江聂安军吕文俊
Owner GUIZHOU LIANSHENG PHARMA
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