A rapid, green and efficient method for extracting silk flavonoids and its detection method

A kind of saflavone and green technology, which is applied in the field of fast, green and efficient extraction of silkworm flavonoids, can solve the problems of not being suitable for large-scale production requirements, cumbersome steps, low safety, etc., to avoid environmental pollution and food safety problems, simple process, and filtration Effect of Difficulty Reduction

Active Publication Date: 2021-09-17
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction method of flavonoids generally adopts organic solvent extraction. Although the extraction rate of flavonoids is high, the safety is low and the steps are cumbersome, which is not suitable for large-scale production requirements.

Method used

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  • A rapid, green and efficient method for extracting silk flavonoids and its detection method
  • A rapid, green and efficient method for extracting silk flavonoids and its detection method
  • A rapid, green and efficient method for extracting silk flavonoids and its detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for fast, green and efficient extraction of silk flavonoids, comprising the following steps:

[0029] (1) Crush the dried silkworm excrement and pass through a 50-mesh sieve;

[0030] (2) Take 120g of dried and crushed silkworm excrement powder, add 2400mL of ultrapure water at a water temperature of 45°C (the ratio of solid to liquid is 1g:20mL), and soak for 30 minutes. Put it into ultrasonic extraction for 70min, pour the filter residue and filtrate into the flash extractor for standby;

[0031] (3) Set up the flash extractor, turn on the switch, raise the knife head, place the solution in step (2) on the operating table, lower the knife head, and the knife head falls to the liquid surface and is 1cm away from the bottom of the beaker (the knife head cannot be in contact with the beaker) bottom contact);

[0032] (4) Be careful not to adjust the gear suddenly in advance of the flash, and adjust the gear slowly to the required gear after the cutter head rot...

Embodiment 2

[0037] A method for fast, green and efficient extraction of silk flavonoids, comprising the following steps:

[0038]According to the steps of Example 1, only the dosage ratio of the pulverized silkworm excrement obtained in step (1) in step (2) and the aqueous solution with a water temperature of 45°C is replaced from 1g:20mL to 1g:25mL, 1g:30mL, 1g :35mL, 1g:40mL, the content of gained silk flavonoids is as follows figure 1 As shown, it is 3.54mg / g, 4.48mg / g, 4.08mg / g, 4.34mg / g in turn.

Embodiment 3

[0040] A method for fast, green and efficient extraction of silk flavonoids, comprising the following steps:

[0041] According to the steps of Example 1, only the ultrasonic extraction time in step (2) is replaced by 70min to 40min, 50min, 60min and 80min, the content of the obtained silk flavonoids is as follows figure 2 As shown, it is 4.00mg / g, 4.08mg / g, 4.28mg / g, 3.38mg / g in turn.

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Abstract

The invention relates to the extraction of active ingredients of traditional Chinese medicines, in particular to a method for rapidly, green and high-efficiency extraction of silk flavonoids and a detection method thereof. The method includes the following steps: collecting and removing impurities from the silkworm excrement, drying and pulverizing to obtain the silkworm excrement powder; adopting a method combined with ultrasonic assistance and flash extraction, extracting the silkworm excrement powder with an aqueous solution, concentrating and drying the extract, and measuring the flavonoid content. Compared with conventional extraction methods, the invention utilizes the combined method of ultrasonic extraction and flash extraction, which not only avoids the damage of heat-labile components, but also has high yield, environmental protection, and significantly improves the transfer rate of active components. Compared with the optimized method of ultrasonic extraction of flavonoids, this method has the flavonoid content increased by 19.2%, the extraction conditions are mild, the use of organic solvents is saved, the extraction agent is environmentally friendly, and the extraction cost is low, and the discarded The reuse of silkworm excrement can not only turn waste into treasure, but also greatly solve the problem of environmental pollution.

Description

technical field [0001] The invention relates to the extraction of active ingredients of traditional Chinese medicines, in particular to a method for rapidly, green and high-efficiency extraction of silk flavonoids and a detection method thereof. Background technique [0002] Silkworm excrement, also known as silkworm excrement, is the feces excreted by the larvae of the silkworm moth, Bombyx mori, and is a commonly used traditional Chinese medicinal material in clinical practice. Sweet and warm in nature, it can dispel wind and dampness, promote blood circulation, relieve pain, and sterilize. It is often used to treat rheumatic arthralgia, numbness, skin eczema itching, cholera, and abdominal pain. Studies have shown that silkworm excrement contains flavonoids, volatile oils, steroids, phenols and terpenes. my country's silkworm excrement resources are very rich, but most of them have not been effectively utilized. The full development and utilization of silkworm excrement ...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): G01N21/31G01N21/33G01N1/28G01N1/30G01N1/34
CPCG01N1/28G01N1/286G01N1/30G01N1/34G01N21/31G01N21/33G01N2021/3196
Inventor时桂芹任菲沈佳鑫侯梦丹
OwnerZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY